scholarly journals Comprehensive Mapping Antigenic Epitopes of NS1 Protein of Japanese Encephalitis Virus with Monoclonal Antibodies

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e67553 ◽  
Author(s):  
Rong-Hong Hua ◽  
Li-Ke Liu ◽  
Zhen-Shi Chen ◽  
Ye-Nan Li ◽  
Zhi-Gao Bu
Keyword(s):  
Monoclonal Antibodies ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
Antigenic Epitopes ◽  
Comprehensive Mapping

scholarly journals Protection of mice against Japanese encephalitis virus group II strain infections by combinations of monoclonal antibodies to different antigenic domains on glycoprotein E

2012 ◽  
Vol 3 (2) ◽  
pp. 17
Author(s):  
Ashok Kumar Gupta ◽  
Attiyaril Abraham Koshy ◽  
Vaibhavi Jawahar Lad
Keyword(s):  
Monoclonal Antibodies ◽  
Protective Effect ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Glycoprotein E ◽  
Antigenic Domains ◽  
Group Ii ◽  
Antigenic Epitopes ◽  
Group 1

A combination of at least three hemagglutination- inhibition-positive (HAI) and virus-specific (Hs) monoclonal antibodies (MAbs) to glycoprotein E (gpE) of Japanese encephalitis virus (JEV) fully protected (100%) mice against JEV strain 733913 infections (group 1). However, these representative epitopes are reported to have been lost on JEV group II strains. In the present study, therefore, the protective effect of various combinations of anti-gpE MAbs representing antigenic epitopes other than Hs was studied on mice infections with JEV group II strains: JEV strains 641686 and 691004. MAbs used in the protective experiments were characterized as HAI-negative virus-specific (NHs) and HAI-positive flavivirus cross-reactive (Hx). Additionally, one of the Hs MAbs (MAb Hs-3) was included in the experiments. Mice were first administered single MAbs or their combinations intraperitoneally and 24 h later, infected with the virus intracerebrally. Protection rates of 70-75% were obtained with a combination of four MAbs: MAbs NHs-1, Hx-1, Hx-3 and Hs-3. However, protection rates of only 20-40% were obtained with three MAbs but none was observed with single or two MAbs. There was, however, a substantial increase in mice survival. The protective effect of several combinations of anti-gpE MAbs representing different antigenic epitopes might be due to the enhancement of binding within the same group and also between different MAb groups. The present results indicate that NHs and Hx epitopes should be incorporated with three Hs epitopes in a JEV vaccine that would have an added advantage, particularly in the flaviviral endemic areas with JEV strain variations.

Protective immunity of E. coli-synthesized NS1 protein of Japanese encephalitis virus

2007 ◽  
Vol 30 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Cheng-Wen Lin ◽  
Kuang-Ting Liu ◽  
Hong-Da Huang ◽  
Wei-June Chen
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Protective Immunity ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
E Coli

scholarly journals The Japanese Encephalitis Virus NS1′ Protein Inhibits Type I IFN Production by Targeting MAVS

2020 ◽  
Vol 204 (5) ◽  
pp. 1287-1298 ◽  
Author(s):  
Dengyuan Zhou ◽  
Qiuyan Li ◽  
Fan Jia ◽  
Luping Zhang ◽  
Shengfeng Wan ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Type I ◽  
Type I Ifn ◽  
Ns1 Protein

scholarly journals Expression and characterization of recombinant Japanese encephalitis virus NS1 protein in Drosophila S2 cell

2008 ◽  
Vol 2 (Suppl 1) ◽  
pp. P36
Author(s):  
Yize Li ◽  
Marie Flamand ◽  
Dorian Counor ◽  
Nelly Kieffer ◽  
Felix Rey ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Ns1 Protein
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