scholarly journals Rapid Focused Sequencing: A Multiplexed Assay for Simultaneous Detection and Strain Typing of Bacillus anthracis, Francisella tularensis, and Yersinia pestis

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56093 ◽  
Author(s):  
Rosemary S. Turingan ◽  
Hans-Ulrich Thomann ◽  
Anna Zolotova ◽  
Eugene Tan ◽  
Richard F. Selden
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Francisella Tularensis ◽  
Simultaneous Detection ◽  
Strain Typing

scholarly journals Comparison of Two Suspension Arrays for Simultaneous Detection of Five Biothreat Bacterial in Powder Samples

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Yu Yang ◽  
Jing Wang ◽  
Haiyan Wen ◽  
Hengchuan Liu
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Simultaneous Detection ◽  
Universal Primers ◽  
Universal Primer ◽  
Suspension Array ◽  
Specific Pathogen ◽  
Powder Samples ◽  
High Conservation ◽  
Species Specific

We have developed novel Bio-Plex assays for simultaneous detection ofBacillus anthracis, Yersinia pestis, Brucella spp., Francisella tularensis,andBurkholderia pseudomallei. Universal primers were used to amplify highly conserved region located within the 16S rRNA amplicon, followed by hybridized to pathogen-specific probes for identification of these five organisms. The other assay is based on multiplex PCR to simultaneously amplify five species-specific pathogen identification-targeted regions unique to individual pathogen. Both of the two arrays are validated to be flexible and sensitive for simultaneous detection of bioterrorism bacteria. However, universal primer PCR-based array could not identifyBacillus anthracis, Yersinia pestis,andBrucella spp.at the species level because of the high conservation of 16S rDNA of the same genus. The two suspension arrays can be utilized to detectBacillus anthracissterne spore andYersinia pestisEV76 from mimic “write powder” samples, they also proved that the suspension array system will be valuable tools for diagnosis of bacterial biothreat agents in environmental samples.

Monitoring biothreat agents (Francisella tularensis, Bacillus anthracis and Yersinia pestis) with a portable real-time PCR instrument

2015 ◽  
Vol 115 ◽  
pp. 89-93 ◽  
Author(s):  
Markos Mölsä ◽  
Heidi Hemmilä ◽  
Anna Katz ◽  
Jukka Niemimaa ◽  
Kristian M. Forbes ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Real Time ◽  
Francisella Tularensis ◽  
Real Time Pcr

scholarly journals Rapid field detection assays for Bacillus anthracis, Brucella spp., Francisella tularensis and Yersinia pestis

2011 ◽  
Vol 17 (1) ◽  
pp. 34-43 ◽  
Author(s):  
P. Matero ◽  
H. Hemmilä ◽  
H. Tomaso ◽  
H. Piiparinen ◽  
K. Rantakokko-Jalava ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Francisella Tularensis ◽  
Field Detection

scholarly journals Rapid Antibiotic Susceptibility Testing of Tier-1 Agents Bacillus anthracis, Yersinia pestis, and Francisella tularensis Directly From Whole Blood Samples

2021 ◽  
Vol 12 ◽  
Author(s):  
Shahar Rotem ◽  
Ohad Shifman ◽  
Moshe Aftalion ◽  
David Gur ◽  
Tamar Aminov ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Francisella Tularensis ◽  
Whole Blood ◽  
Antibiotic Susceptibility ◽  
Blood Samples ◽  
Wide Range ◽  
Tier 1 ◽  
Whole Blood Samples ◽  
Susceptibility Tests

Rapid antibiotic susceptibility tests, performed directly on whole blood samples, will offer great clinical advantages. This issue is of considerable importance when it comes to bioterror pathogens where prompt antibiotic treatment should be offered to infected patients as well as prophylaxis to suspected exposed individuals. Herein, we describe a novel and rapid method, named MAPt, that is based on the direct application of a blood sample onto solid agar that has been embedded with different concentrations of the tested antibiotic. Following a short incubation, bacterial growth is monitored by qPCR. The method was applied on blood cultures and whole blood samples inoculated with the Tier-1 pathogens Bacillus anthracis, Yersinia pestis, and Francisella tularensis. The use of agar medium, which better supports the growth of bacteria at low concentrations, together with the use of qPCR, which provides sensitivity and specificity, allowed minimal inhibitory concentration (MIC) determination to a wide range of bacterial concentrations, ranging from ∼5 × 102 cfu/ml up to 108 cfu/ml. The omission of the enrichment procedure in blood culture and the isolation step, both required in standard antibiotic susceptibility tests (ASTs), allowed a dramatic reduction in time to answer, from a few days to a few hours. The total time required for MIC determination was ∼6 h for fast-growing bacteria, such as B. anthracis, and 12–16 h for slow-growing bacteria, represented by Y. pestis and F. tularensis. Accordingly, MAPt may offer health authorities means for public preparedness in the case of a bioterror attack as well as prompt clinical treatment options in common blood stream infections.

Duplex Lateral Flow Assay for the Simultaneous Detection of Yersinia pestis and Francisella tularensis

2018 ◽  
Vol 90 (21) ◽  
pp. 12745-12751 ◽  
Author(s):  
Miriam Jauset-Rubio ◽  
Herbert Tomaso ◽  
Mohammad S. El-Shahawi ◽  
Abdulaziz S. Bashammakh ◽  
Abdulrahman O. Al-Youbi ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Francisella Tularensis ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay

Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis

2007 ◽  
Vol 26 (3) ◽  
pp. 207-211 ◽  
Author(s):  
T. Skottman ◽  
H. Piiparinen ◽  
H. Hyytiäinen ◽  
V. Myllys ◽  
M. Skurnik ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Real Time ◽  
Francisella Tularensis ◽  
Real Time Pcr ◽  
Pcr Detection
Sign in / Sign up

Export Citation Format

Share Document