scholarly journals Functional Innervation of Guinea-Pig Bladder Interstitial Cells of Cajal Subtypes: Neurogenic Stimulation Evokes In Situ Calcium Transients

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53423 ◽  
Author(s):  
Susannah M. Gray ◽  
J. Graham McGeown ◽  
Gordon McMurray ◽  
Karen D. McCloskey
Keyword(s):  
Guinea Pig ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Calcium Transients ◽  
Cells Of Cajal

Ultrastructural Properties of Interstitial Cells of Cajal in the Guinea Pig Bladder

2011 ◽  
Vol 185 (3) ◽  
pp. 1123-1131 ◽  
Author(s):  
Rebecca M.J. Cunningham ◽  
Patrick Larkin ◽  
Karen D. McCloskey
Keyword(s):  
Guinea Pig ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal

Region-specific distribution of the P2Y4 receptor in enteric glial cells and interstitial cells of Cajal within the guinea-pig gastrointestinal tract

2006 ◽  
Vol 126-127 ◽  
pp. 299-306 ◽  
Author(s):  
Luc Van Nassauw ◽  
Anna Costagliola ◽  
Joeri Van Op den Bosch ◽  
Aldo Cecio ◽  
Jean-Marie Vanderwinden ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Guinea Pig ◽  
Glial Cells ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Specific Distribution ◽  
Enteric Glial Cells ◽  
Cells Of Cajal ◽  
P2y4 Receptor

scholarly journals Cholinergic-induced Ca2+ signaling in interstitial cells of Cajal from the guinea pig bladder

2008 ◽  
Vol 294 (3) ◽  
pp. F645-F655 ◽  
Author(s):  
Louise Johnston ◽  
Chris Carson ◽  
Alan D. Lyons ◽  
Ross A. Davidson ◽  
Karen D. McCloskey
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Receptor Antagonist ◽  
Interstitial Cells Of Cajal ◽  
Cell Types ◽  
Interstitial Cells ◽  
Isolated Cells ◽  
Dissociated Cells ◽  
Bladder Detrusor ◽  
Cells Of Cajal

Acetylcholine released from parasympathetic excitatory nerves activates contraction in detrusor smooth muscle. Immunohistochemical labeling of guinea pig detrusor with anti- c-Kit and anti-VAChT demonstrated a close structural relationship between interstitial cells of Cajal (ICC) and cholinergic nerves. The ability of guinea pig bladder detrusor ICC to respond to the acetylcholine analog, carbachol, was investigated in enzymatically dissociated cells, loaded with the Ca2+ indicator fluo 4AM. ICC fired Ca2+ transients in response to stimulation by carbachol (1/10 μM). Their pharmacology was consistent with carbachol-induced contractions in strips of detrusor which were inhibited by 4-DAMP (1 μM), an M3 receptor antagonist, but not by the M2 receptor antagonist methoctramine (1 μM). The source of Ca2+ underlying the carbachol transients in isolated ICC was investigated using agents to interfere with influx or release from intracellular stores. Nifedipine (1 μM) or Ni2+ (30–100 μM) to block Ca2+ channels or the removal of external Ca2+ reduced the amplitude of the carbachol transients. Application of ryanodine (30 μM) or tetracaine (100 μM) abolished the transients. The phospholipase C inhibitor, U-73122 (2.5 μM), significantly reduced the responses. 2-Aminoethoxydiethylborate (30 μM) caused a significant reduction and Xestospongin C (1 μM) was more effective, almost abolishing the responses. Intact in situ preparations of guinea pig bladder loaded with a Ca2+ indicator showed distinctively different patterns of spontaneous Ca2+ events in smooth muscle cells and ICC. Both cell types responded to carbachol by an increase in frequency of these events. In conclusion, guinea pig bladder detrusor ICC, both as isolated cells and within whole tissue preparations, respond to cholinergic stimulation by firing Ca2+ transients.

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