scholarly journals Reduced Proficiency in Homologous Recombination Underlies the High Sensitivity of Embryonal Carcinoma Testicular Germ Cell Tumors to Cisplatin and Poly (ADP-Ribose) Polymerase Inhibition

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51563 ◽  
Author(s):  
Francesca Cavallo ◽  
Grazia Graziani ◽  
Cristina Antinozzi ◽  
Darren R. Feldman ◽  
Jane Houldsworth ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Germ Cell ◽  
Embryonal Carcinoma ◽  
Germ Cell Tumors ◽  
High Sensitivity ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell

scholarly journals Aggrus: a diagnostic marker that distinguishes seminoma from embryonal carcinoma in testicular germ cell tumors

Oncogene ◽  
2004 ◽  
Vol 23 (52) ◽  
pp. 8552-8556 ◽  
Author(s):  
Yukinari Kato ◽  
Isoji Sasagawa ◽  
Mika Kaneko ◽  
Motoki Osawa ◽  
Naoya Fujita ◽  
...  
Keyword(s):  
Germ Cell ◽  
Embryonal Carcinoma ◽  
Diagnostic Marker ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell

scholarly journals ScaR—a tool for sensitive detection of known fusion transcripts: establishing prevalence of fusions in testicular germ cell tumors

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Sen Zhao ◽  
Andreas M Hoff ◽  
Rolf I Skotheim
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Simulated Data ◽  
High Sensitivity ◽  
Fusion Transcript ◽  
Data Sets ◽  
Testicular Germ Cell Tumors ◽  
Sequencing Data ◽  
Testicular Germ Cell ◽  
Fusion Transcripts

Abstract Bioinformatics tools for fusion transcript detection from RNA-sequencing data are in general developed for identification of novel fusions, which demands a high number of supporting reads and strict filters to avoid false discoveries. As our knowledge of bona fide fusion genes becomes more saturated, there is a need to establish their prevalence with high sensitivity. We present ScaR, a tool that uses a supervised scaffold realignment approach for sensitive fusion detection in RNA-seq data. ScaR detects a set of 130 synthetic fusion transcripts from simulated data at a higher sensitivity compared to established fusion finders. Applied to fusion transcripts potentially involved in testicular germ cell tumors (TGCTs), ScaR detects the fusions RCC1-ABHD12B and CLEC6A-CLEC4D in 9% and 28% of 150 TGCTs, respectively. The fusions were not detected in any of 198 normal testis tissues. Thus, we demonstrate high prevalence of RCC1-ABHD12B and CLEC6A-CLEC4D in TGCTs, and their cancer specific features. Further, we find that RCC1-ABHD12B and CLEC6A-CLEC4D are predominantly expressed in the seminoma and embryonal carcinoma histological subtypes of TGCTs, respectively. In conclusion, ScaR is useful for establishing the frequency of known and validated fusion transcripts in larger data sets and detecting clinically relevant fusion transcripts with high sensitivity.

Abstract C51: High sensitivity of testicular germ cell tumors to PARP inhibitor olaparib alone and in combination with cisplatin

2011 ◽  
Author(s):  
Francesca Cavallo ◽  
Grazia Graziani ◽  
Cristina Antinozzi ◽  
Raju Chaganti ◽  
George J. Bosl ◽  
...  
Keyword(s):  
Germ Cell ◽  
Parp Inhibitor ◽  
Germ Cell Tumors ◽  
High Sensitivity ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell

An Overview of Testicular Germ Cell Tumors

2007 ◽  
Vol 131 (8) ◽  
pp. 1267-1280 ◽  
Author(s):  
Armita Bahrami ◽  
Jae Y. Ro ◽  
Alberto G. Ayala
Keyword(s):  
Germ Cell ◽  
Embryonal Carcinoma ◽  
Germ Cell Tumors ◽  
Stage I ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Common Component ◽  
Clinical Behavior ◽  
Pathologic Features ◽  
Spermatocytic Seminoma

Abstract Context.—More than 90% of testicular neoplasms originate from germ cells. Testicular germ cell tumors (GCTs) are a heterogeneous group of neoplasms with diverse histopathology and clinical behavior. Objective.—To help the readers distinguish various subtypes of GCTs, to highlight the clinical manifestations and pathologic features of these tumors, and to review several newly developed immunohistochemical markers for GCTs. Data Sources.—Review of the pertinent literature and our experience. Conclusions.—The etiology of GCTs is largely unknown. Cytogenetic studies suggest a different pathogenesis for each group of infantile/prepubertal GCTs, postpubertal GCTs, and spermatocytic seminoma. Unclassified intratubular germ cell neoplasia is the precursor of all GCTs, excluding spermatocytic seminoma and infantile/prepubertal GCTs. Seminoma, the most common GCT in adults, does not occur before 5 years of age. Spermatocytic seminoma, a tumor of elderly men, typically has an indolent clinical behavior, but rarely it undergoes sarcomatous transformation associated with an aggressive behavior. Embryonal carcinoma is the most common component in mixed GCTs. Eighty percent or more of embryonal carcinoma component and vascular invasion are recognized predictors of occult metastasis for clinical stage I mixed GCTs. Most patients with prepubertal yolk sac tumor, the most common pediatric GCT, have stage I disease at presentation. Most choriocarcinomas present with metastatic symptoms because of the propensity for rapid hematogenous dissemination. Teratomas in children regardless of maturity and dermoid cysts in adults are benign; in contrast, teratomas in adults have a malignant behavior. With appropriate therapy, the majority of testicular GCTs are curable.

scholarly journals ScaR - A tool for sensitive detection of known fusion transcripts: Establishing prevalence of fusions in testicular germ cell tumors

2019 ◽  
Author(s):  
Sen Zhao ◽  
Andreas M. Hoff ◽  
Rolf I. Skotheim
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Simulated Data ◽  
High Sensitivity ◽  
Fusion Transcript ◽  
Data Sets ◽  
Testicular Germ Cell Tumors ◽  
Sequencing Data ◽  
Testicular Germ Cell ◽  
Fusion Transcripts

AbstractBioinformatics tools for fusion transcript detection from RNA-sequencing data are in general developed for identification of novel fusions, which demands a high number of supporting reads and strict filters to avoid false discoveries. As our knowledge of bona-fide fusion genes becomes more saturated, there is a need to establish their prevalence with high sensitivity. We present ScaR, a tool that uses a scaffold realignment approach for sensitive fusion detection in RNA-seq data. ScaR detects a set of 50 synthetic fusion transcripts from simulated data at a higher sensitivity compared to established fusion finders. Applied to fusion transcripts potentially involved in testicular germ cell tumors (TGCTs), ScaR detects the fusions RCC1-ABHD12B and CLEC6A-CLEC4D in 9% and 28% of 150 TGCTs, respectively. The fusions were not detected in any of 198 normal testis tissues. Thus, we demonstrate high prevalence of RCC1-ABHD12B and CLEC6A-CLEC4D in TGCTs, and their cancer specific features. Further, we find that RCC1-ABHD12B and CLEC6A-CLEC4D are predominantly expressed in the seminoma and embryonal carcinoma histological subtypes of TGCTs, respectively. In conclusion, ScaR is useful for establishing the frequency of known fusion transcripts in larger data sets and detecting clinically relevant fusion transcripts with high sensitivity.Availabilityhttps://github.com/senzhaocode/ScaR

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