scholarly journals One out of Four: HspL but No Other Small Heat Shock Protein of Agrobacterium tumefaciens Acts as Efficient Virulence-Promoting VirB8 Chaperone

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49685 ◽  
Author(s):  
Yun-Long Tsai ◽  
Yin-Ru Chiang ◽  
Chih-Feng Wu ◽  
Franz Narberhaus ◽  
Erh-Min Lai
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Small Heat Shock Protein

scholarly journals Small heat-shock protein HspL is induced by VirB protein(s) and promotes VirB/D4-mediated DNA transfer in Agrobacterium tumefaciens

Microbiology ◽  
2009 ◽  
Vol 155 (10) ◽  
pp. 3270-3280 ◽  
Author(s):  
Yun-Long Tsai ◽  
Ming-Hsuan Wang ◽  
Chan Gao ◽  
Sonja Klüsener ◽  
Christian Baron ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Response Regulator ◽  
Small Heat Shock Protein ◽  
Protein S ◽  
Dna Transfer ◽  
Protein Accumulation ◽  
Crown Gall Disease ◽  
Type Iv

Agrobacterium tumefaciens is a Gram-negative plant-pathogenic bacterium that causes crown gall disease by transferring and integrating its transferred DNA (T-DNA) into the host genome. We characterized the chromosomally encoded alpha-crystallin-type small heat-shock protein (α-Hsp) HspL, which was induced by the virulence (vir) gene inducer acetosyringone (AS). The transcription of hspL but not three other α-Hsp genes (hspC, hspAT1, hspAT2) was upregulated by AS. Further expression analysis in various vir mutants suggested that AS-induced hspL transcription is not directly activated by the VirG response regulator but rather depends on the expression of VirG-activated virB genes encoding components of the type IV secretion system (T4SS). Among the 11 virB genes encoded by the virB operon, HspL protein levels were reduced in strains with deletions of virB6, virB8 or virB11. VirB protein accumulation but not virB transcription levels were reduced in an hspL deletion mutant early after AS induction, implying that HspL may affect the stability of individual VirB proteins or of the T4S complex directly or indirectly. Tumorigenesis efficiency and the VirB/D4-mediated conjugal transfer of an IncQ plasmid RSF1010 derivative between A. tumefaciens strains were reduced in the absence of HspL. In conclusion, increased HspL abundance is triggered in response to certain VirB protein(s) and plays a role in optimal VirB protein accumulation, VirB/D4-mediated DNA transfer and tumorigenesis.

Mutant small heat shock protein B3 causes motor neuropathy: Utility of a candidate gene approach

Neurology ◽  
2010 ◽  
Vol 74 (6) ◽  
pp. 502-506 ◽  
Author(s):  
S. J. Kolb ◽  
P. J. Snyder ◽  
E. J. Poi ◽  
E. A. Renard ◽  
A. Bartlett ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Candidate Gene ◽  
Small Heat Shock Protein ◽  
Candidate Gene Approach ◽  
Motor Neuropathy

Evolution and functional diversification of the small heat shock protein/α-crystallin family in higher plants

Planta ◽  
2011 ◽  
Vol 235 (6) ◽  
pp. 1299-1313 ◽  
Author(s):  
Hernán Gabriel Bondino ◽  
Estela Marta Valle ◽  
Arjen ten Have
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Higher Plants ◽  
Small Heat Shock Protein ◽  
Functional Diversification

The small heat shock protein (HSP) 20 is dynamically associated with the actin cross-linking protein actinin

2003 ◽  
Vol 111 (1) ◽  
pp. 152-157 ◽  
Author(s):  
Deron J Tessier ◽  
Padmini Komalavilas ◽  
Alyssa Panitch ◽  
Lokesh Joshi ◽  
Colleen M Brophy
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Small Heat Shock Protein ◽  
Cross Linking

Elevated Retina-Specific Expression of the Small Heat Shock Protein, αA-crystallin, Is Associated with Photoreceptor Protection in Experimental Uveitis

2008 ◽  
Vol 49 (3) ◽  
pp. 1161 ◽  
Author(s):  
Narsing A. Rao ◽  
Sindhu Saraswathy ◽  
Guey Shuang Wu ◽  
George S. Katselis ◽  
Eric F. Wawrousek ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Small Heat Shock Protein ◽  
Specific Expression
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