scholarly journals Protective and Therapeutic Efficacy of Mycobacterium smegmatis Expressing HBHA-hIL12 Fusion Protein against Mycobacterium tuberculosis in Mice

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31908 ◽  
Author(s):  
Shanmin Zhao ◽  
Yong Zhao ◽  
Fengfeng Mao ◽  
Caiqin Zhang ◽  
Bing Bai ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Fusion Protein ◽  
Mycobacterium Smegmatis ◽  
Therapeutic Efficacy

Mycobacterium tuberculosis Ag85b:hfcγ1 recombinant fusion protein as a selective receptor-dependent delivery system for antigen presentation

2019 ◽  
Vol 129 ◽  
pp. 68-73 ◽  
Author(s):  
Mohsen Karbalaei Zadeh Babaki ◽  
Mahboubeh Taghiabadi ◽  
Saman Soleimanpour ◽  
Masoud Saleh Moghadam ◽  
Arman Mosavat ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Fusion Protein ◽  
Antigen Presentation ◽  
Delivery System ◽  
Recombinant Fusion Protein

scholarly journals Expression of Mycobacterium smegmatisPyrazinamidase in Mycobacterium tuberculosis Confers Hypersensitivity to Pyrazinamide and Related Amides

2000 ◽  
Vol 182 (19) ◽  
pp. 5479-5485 ◽  
Author(s):  
Helena I. M. Boshoff ◽  
Valerie Mizrahi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Smegmatis ◽  
Intracellular Localization ◽  
Wild Type ◽  
Gene Encoding ◽  
Allelic Exchange ◽  
Mutant Strains ◽  
Established Role ◽  
Amidase Gene

ABSTRACT A pyrazinamidase (PZase)-deficient pncA mutant ofMycobacterium tuberculosis, constructed by allelic exchange, was used to investigate the effects of heterologous amidase gene expression on the susceptibility of this organism to pyrazinamide (PZA) and related amides. The mutant was highly resistant to PZA (MIC, >2,000 μg/ml), in accordance with the well-established role ofpncA in the PZA susceptibility of M. tuberculosis (A. Scorpio and Y. Zhang, Nat. Med. 2:662–667, 1996). Integration of the pzaA gene encoding the major PZase/nicotinamidase from Mycobacterium smegmatis (H. I. M. Boshoff and V. Mizrahi, J. Bacteriol. 180:5809–5814, 1998) or the M. tuberculosis pncA gene into the pncAmutant complemented its PZase/nicotinamidase defect. In bothpzaA- and pncA-complemented mutant strains, the PZase activity was detected exclusively in the cytoplasm, suggesting an intracellular localization for PzaA and PncA. ThepzaA-complemented strain was hypersensitive to PZA (MIC, ≤10 μg/ml) and nicotinamide (MIC, ≥20 μg/ml) and was also sensitive to benzamide (MIC, 20 μg/ml), unlike the wild-type andpncA-complemented mutant strains, which were highly resistant to this amide (MIC, >500 μg/ml). This finding was consistent with the observation that benzamide is hydrolyzed by PzaA but not by PncA. Overexpression of PzaA also conferred sensitivity to PZA, nicotinamide, and benzamide on M. smegmatis (MIC, 150 μg/ml in all cases) and rendered Escherichia colihypersensitive for growth at low pH.

scholarly journals Therapeutic Potential of Human Neutrophil Peptide 1 against Experimental Tuberculosis

2001 ◽  
Vol 45 (2) ◽  
pp. 639-640 ◽  
Author(s):  
Sudhir Sharma ◽  
Indu Verma ◽  
G. K. Khuller
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Human Neutrophil ◽  
Therapeutic Efficacy ◽  
Therapeutic Potential ◽  
Therapeutic Activity ◽  
Experimental Tuberculosis ◽  
Dose Dependent ◽  
Different Doses ◽  
Human Neutrophil Peptide

ABSTRACT The therapeutic efficacy of human neutrophil peptide 1 (HNP-1) against experimental tuberculosis in mice on the basis of numbers of CFU has been examined. Mice infected with 1.5 × 104CFU of Mycobacterium tuberculosisH37Rv and treated with different doses of HNP-1 injected subcutaneously exhibited significant clearance of bacilli from lungs, livers, and spleens. There were time- and dose-dependent decreases in the bacillary load in lungs, livers, and spleens of the HNP-1-treated animals compared to that in controls (untreated animals). These observations strongly suggest the therapeutic activity of HNP-1 against tuberculosis.

scholarly journals Effects of clofazimine on planktonic and biofilm growth of Mycobacterium tuberculosis and Mycobacterium smegmatis

2015 ◽  
Vol 3 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Maborwa T. Mothiba ◽  
Ronald Anderson ◽  
Bernard Fourie ◽  
Willem A. Germishuizen ◽  
Moloko C. Cholo
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Smegmatis ◽  
Biofilm Growth
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