scholarly journals Assessment of Inhibitors of Pathogenic Crimean-Congo Hemorrhagic Fever Virus Strains Using Virus-Like Particles

2015 ◽  
Vol 9 (12) ◽  
pp. e0004259 ◽  
Author(s):  
Marko Zivcec ◽  
Maureen G. Metcalfe ◽  
César G. Albariño ◽  
Lisa W. Guerrero ◽  
Scott D. Pegan ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Virus Like Particles ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

scholarly journals Crimean-Congo hemorrhagic fever virus strains Hoti and Afghanistan cause viremia and mild clinical disease in cynomolgus monkeys

2020 ◽  
Vol 14 (8) ◽  
pp. e0008637 ◽  
Author(s):  
Robert W. Cross ◽  
Abhishek N. Prasad ◽  
Viktoriya Borisevich ◽  
Joan B. Geisbert ◽  
Krystle N. Agans ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Clinical Disease ◽  
Cynomolgus Monkeys ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

scholarly journals Co-circulation of Crimean-Congo Hemorrhagic Fever virus strains Asia 1 and 2 between the border of Iran and Pakistan

Heliyon ◽  
2017 ◽  
Vol 3 (11) ◽  
pp. e00439 ◽  
Author(s):  
Nariman Shahhosseini ◽  
Ahmad Jafarbekloo ◽  
Zakkyeh Telmadarraiy ◽  
Sadegh Chinikar ◽  
Ali Haeri ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

scholarly journals Spatial and Phylodynamic Survey on Crimean-Congo Hemorrhagic Fever Virus Strains in Northeast of Iran

2018 ◽  
Vol 11 (3) ◽  
Author(s):  
Faezeh Faghihi ◽  
Zakkyeh Telmadarraiy ◽  
Sadegh Chinikar ◽  
Norbert Nowotny ◽  
Anthony R. Fooks ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

Genetic analysis of the M RNA segment of Crimean-Congo hemorrhagic fever virus strains involved in the recent outbreaks in Russia

2004 ◽  
Vol 149 (11) ◽  
pp. 2199-2213 ◽  
Author(s):  
J. H. Kuhn ◽  
S. V. Seregin ◽  
S. P. Morzunov ◽  
I. D. Petrova ◽  
O. I. Vyshemirskii ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

Phylogeny of tick-derived Crimean-Congo hemorrhagic fever virus strains in Iran

2016 ◽  
Vol 7 (6) ◽  
pp. 1216-1221 ◽  
Author(s):  
Peyvand Biglari ◽  
Sadegh Chinikar ◽  
Hamid Belqeiszadeh ◽  
Zakkyeh Telmadarraiy ◽  
Ehsan Mostafavi ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

Genetic Characterization of the M RNA Segment of Crimean-Congo Hemorrhagic Fever Virus Strains Isolated in Russia and Tajikistan

Virus Genes ◽  
2004 ◽  
Vol 28 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Sergei V. Seregin ◽  
Evgeny I. Samokhvalov ◽  
Irina D. Petrova ◽  
Oleg I. Vyshemirskii ◽  
Ekaterina G. Samokhvalova ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

scholarly journals A Virus-Like Particle System Identifies the Endonuclease Domain of Crimean-Congo Hemorrhagic Fever Virus

2015 ◽  
Vol 89 (11) ◽  
pp. 5957-5967 ◽  
Author(s):  
Stephanie Devignot ◽  
Eric Bergeron ◽  
Stuart Nichol ◽  
Ali Mirazimi ◽  
Friedemann Weber
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Particle Assembly ◽  
Content Type ◽  
Virus Like Particles ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Like Particle ◽  
Endonuclease Domain ◽  
Biosafety Level

ABSTRACTCrimean-Congo hemorrhagic fever virus(CCHFV; genusNairovirus) is an extremely pathogenic member of theBunyaviridaefamily. Since handling of the virus requires a biosafety level 4 (BSL-4) facility, little is known about pathomechanisms and host interactions. Here, we describe the establishment of a transcriptionally competent virus-like particle (tc-VLP) system for CCHFV. Recombinant polymerase (L), nucleocapsid protein (N) and a reporter minigenome expressed in human HuH-7 cells resulted in formation of transcriptionally active nucleocapsids that could be packaged by coexpressed CCHFV glycoproteins into tc-VLPs. The tc-VLPs resembled authentic virus particles in their protein composition and neutralization sensitivity to anti-CCHFV antibodies and could recapitulate all steps of the viral replication cycle. Particle attachment, entry, and primary transcription were modeled by infection of naive cells. The subsequent steps of genome replication, secondary transcription, and particle assembly and release can be obtained upon passaging the tc-VLPs on cells expressing CCHFV structural proteins. The utility of the VLP system was demonstrated by showing that the endonuclease domain of L is located around amino acid D693, as was predictedin silicoby B. Morin et al. (PLoS Pathog 6:e1001038, 2010,http://dx.doi.org/10.1371/journal.ppat.1001038). The tc-VLP system will greatly facilitate studies and diagnostics of CCHFV under non-BSL-4 conditions.IMPORTANCECrimean-Congo hemorrhagic fever virus (CCHFV) is an extremely virulent pathogen of humans. Since the virus can be handled only at the highest biosafety level, research is restricted to a few specialized laboratories. We developed a plasmid-based system to produce virus-like particles with the ability to infect cells and transcribe a reporter genome. Due to the absence of viral genes, the virus-like particles are unable to spread or cause disease, thus allowing study of aspects of CCHFV biology under relaxed biosafety conditions.

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