scholarly journals Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana

2010 ◽  
Vol 4 (7) ◽  
pp. e751 ◽  
Author(s):  
Katharina Röltgen ◽  
Weihong Qi ◽  
Marie-Thérèse Ruf ◽  
Ernestina Mensah-Quainoo ◽  
Sacha J. Pidot ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Buruli Ulcer ◽  
Mycobacterium Ulcerans ◽  
Nucleotide Polymorphism ◽  
Endemic Region ◽  
Single Nucleotide ◽  
Single Nucleotide Polymorphism Typing

scholarly journals Insertion Sequence Element Single Nucleotide Polymorphism Typing Provides Insights into the Population Structure and Evolution of Mycobacterium ulcerans across Africa

2013 ◽  
Vol 80 (3) ◽  
pp. 1197-1209 ◽  
Author(s):  
Koen Vandelannoote ◽  
Kurt Jordaens ◽  
Pieter Bomans ◽  
Herwig Leirs ◽  
Lies Durnez ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Population Structure ◽  
Insertion Sequence ◽  
Buruli Ulcer ◽  
Mycobacterium Ulcerans ◽  
Nucleotide Polymorphism ◽  
Sequence Element ◽  
Single Nucleotide ◽  
Content Type ◽  
Insertion Sequence Element

ABSTRACTBuruli ulcer is an indolent, slowly progressing necrotizing disease of the skin caused by infection withMycobacterium ulcerans. In the present study, we applied a redesigned technique to a vast panel ofM. ulceransdisease isolates and clinical samples originating from multiple African disease foci in order to (i) gain fundamental insights into the population structure and evolutionary history of the pathogen and (ii) disentangle the phylogeographic relationships within the genetically conserved cluster of AfricanM. ulcerans. Our analyses identified 23 different African insertion sequence element single nucleotide polymorphism (ISE-SNP) types that dominate in different areas where Buruli ulcer is endemic. These ISE-SNP types appear to be the initial stages of clonal diversification from a common, possibly ancestral ISE-SNP type. ISE-SNP types were found unevenly distributed over the greater West African hydrological drainage basins. Our findings suggest that geographical barriers bordering the basins to some extent prevented bacterial gene flow between basins and that this resulted in independent focal transmission clusters associated with the hydrological drainage areas. Different phylogenetic methods yielded two well-supported sister clades within the African ISE-SNP types. The ISE-SNP types from the “pan-African clade” were found to be widespread throughout Africa, while the ISE-SNP types of the “Gabonese/Cameroonian clade” were much rarer and found in a more restricted area, which suggested that the latter clade evolved more recently. Additionally, the Gabonese/Cameroonian clade was found to form a strongly supported monophyletic group with Papua New Guinean ISE-SNP type 8, which is unrelated to other Southeast Asian ISE-SNP types.

Automated single nucleotide polymorphism typing using bead array in capillary tube

2010 ◽  
Vol 110 (4) ◽  
pp. 505-508 ◽  
Author(s):  
Yasuo Kagawa ◽  
Mami Hiraoka ◽  
Yukiko Miyashita-Hatano ◽  
Madoka Shishido-Oki ◽  
Mamiko Yoshida ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Capillary Tube ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Single Nucleotide Polymorphism Typing

scholarly journals High-Throughput Genetic Mapping of Mutants via Quantitative Single Nucleotide Polymorphism Typing

Genetics ◽  
2009 ◽  
Vol 184 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Sanzhen Liu ◽  
Hsin D. Chen ◽  
Irina Makarevitch ◽  
Rebecca Shirmer ◽  
Scott J. Emrich ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Genetic Mapping ◽  
High Throughput ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Single Nucleotide Polymorphism Typing
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