scholarly journals Correction: The translesion DNA polymerases Pol ζ and Rev1 are activated independently of PCNA ubiquitination upon UV radiation in mutants of DNA polymerase δ

PLoS Genetics ◽  
2018 ◽  
Vol 14 (2) ◽  
pp. e1007236
Author(s):  
Carine Tellier-Lebegue ◽  
Eléa Dizet ◽  
Emilie Ma ◽  
Xavier Veaute ◽  
Eric Coïc ◽  
...  
Keyword(s):  
Uv Radiation ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Dna Polymerase Δ ◽  
Translesion Dna

scholarly journals The translesion DNA polymerases Pol ζ and Rev1 are activated independently of PCNA ubiquitination upon UV radiation in mutants of DNA polymerase δ

PLoS Genetics ◽  
2017 ◽  
Vol 13 (12) ◽  
pp. e1007119 ◽  
Author(s):  
Carine Tellier-Lebegue ◽  
Eléa Dizet ◽  
Emilie Ma ◽  
Xavier Veaute ◽  
Eric Coïc ◽  
...  
Keyword(s):  
Uv Radiation ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Dna Polymerase Δ ◽  
Translesion Dna

scholarly journals Role of Translesion Synthesis DNA Polymerases in DNA Replication in the Presence of a Weak DNA Polymerase δ in Saccharomyces cerevisiae

2018 ◽  
Vol 8 (2) ◽  
pp. 754-754
Author(s):  
Likui Zhang ◽  
Yanchao Huang ◽  
Xinyuan Zhu ◽  
Yuxiao Wang ◽  
Haoqiang Shi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Dna Polymerase Δ

scholarly journals Role of Translesion Synthesis DNA Polymerases in DNA Replication in the Presence of a Weak DNA Polymerase δ in Saccharomyces cerevisiae

2017 ◽  
Vol 8 (2) ◽  
pp. 754-754
Author(s):  
Likui Zhang ◽  
Yanchao Huang ◽  
Xinyuan Zhu ◽  
Yuxiao Wang ◽  
Haoqiang Shi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Dna Polymerase Δ

scholarly journals PCNA Mono-Ubiquitination and Activation of Translesion DNA Polymerases by DNA Polymerase α

2009 ◽  
Vol 146 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Motoshi Suzuki ◽  
Atsuko Niimi ◽  
Siripan Limsirichaikul ◽  
Shuta Tomida ◽  
Qin Miao Huang ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerases ◽  
Dna Polymerase Α ◽  
Translesion Dna

Interactions of 1-[(S)-3-Hydroxy-2-(phosphonomethoxy)propyl]cytosine (Cidofovir) Diphosphate with DNA Polymerases α, δ and ε*

2001 ◽  
Vol 66 (11) ◽  
pp. 1698-1706 ◽  
Author(s):  
Gabriel Birkuš ◽  
Ivan Votruba ◽  
Miroslav Otmar ◽  
Antonín Holý
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerases ◽  
Competitive Inhibitor ◽  
Dna Polymerase Δ ◽  
Eukaryotic Dna

The inhibitory and/or substrate activity of 1-[(S)-3-hydroxy-2-(phosphonomethoxy)propyl]cytosine [(S)-HPMPC, cidofovir, Vistide™] diphosphate towards eukaryotic DNA polymerases α, δ and ε* was examined. Cidofovir diphosphate is a weak competitive inhibitor of the above enzymes, approximately 3 to 7 times weaker than its adenine analogue (S)-HPMPApp. The enzymes also catalyze incorporation of (S)-HPMPC into DNA; after insertion of one (S)-HPMPC residue into DNA, another dNMP residue may incorporate. DNA polymerase δ and ε* can successively accommodate in the growing chain two (S)-HPMPC residues at the maximum, whereas pol α up to three residues.

scholarly journals Transcriptional Modulator NusA Interacts with Translesion DNA Polymerases in Escherichia coli

2008 ◽  
Vol 191 (2) ◽  
pp. 665-672 ◽  
Author(s):  
Susan E. Cohen ◽  
Veronica G. Godoy ◽  
Graham C. Walker
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Temperature Sensitivity ◽  
Dna Polymerases ◽  
Rna Polymerases ◽  
Translesion Dna Synthesis ◽  
Catalytic Activities ◽  
Translesion Dna

ABSTRACT NusA, a modulator of RNA polymerase, interacts with the DNA polymerase DinB. An increased level of expression of dinB or umuDC suppresses the temperature sensitivity of the nusA11 strain, requiring the catalytic activities of these proteins. We propose that NusA recruits translesion DNA synthesis (TLS) polymerases to RNA polymerases stalled at gaps, coupling TLS to transcription.

scholarly journals Palm Mutants in DNA Polymerases α and η Alter DNA Replication Fidelity and Translesion Activity

2004 ◽  
Vol 24 (7) ◽  
pp. 2734-2746 ◽  
Author(s):  
Atsuko Niimi ◽  
Siripan Limsirichaikul ◽  
Shonen Yoshida ◽  
Shigenori Iwai ◽  
Chikahide Masutani ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Pyrimidine Dimer ◽  
Replication Fidelity ◽  
Dna Polymerase Α ◽  
Replication Errors ◽  
Translesion Dna

ABSTRACT We isolated active mutants in Saccharomyces cerevisiae DNA polymerase α that were associated with a defect in error discrimination. Among them, L868F DNA polymerase α has a spontaneous error frequency of 3 in 100 nucleotides and 570-fold lower replication fidelity than wild-type (WT) polymerase α. In vivo, mutant DNA polymerases confer a mutator phenotype and are synergistic with msh2 or msh6, suggesting that DNA polymerase α-dependent replication errors are recognized and repaired by mismatch repair. In vitro, L868F DNA polymerase α catalyzes efficient bypass of a cis-syn cyclobutane pyrimidine dimer, extending the 3′ T 26,000-fold more efficiently than the WT. Phe34 is equivalent to residue Leu868 in translesion DNA polymerase η, and the F34L mutant of S. cerevisiae DNA polymerase η has reduced translesion DNA synthesis activity in vitro. These data suggest that high-fidelity DNA synthesis by DNA polymerase α is required for genomic stability in yeast. The data also suggest that the phenylalanine and leucine residues in translesion and replicative DNA polymerases, respectively, might have played a role in the functional evolution of these enzyme classes.

scholarly journals PCNA Mono-Ubiquitination and Activation of Translesion DNA Polymerases by DNA Polymerase  

2010 ◽  
Vol 148 (2) ◽  
pp. 261-261
Author(s):  
M. Suzuki ◽  
A. Niimi ◽  
S. Limsirichaikul ◽  
S. Tomida ◽  
Q. M. Huang ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerases ◽  
Translesion Dna

scholarly journals Plant DNA polymerases α and δ mediate replication of geminiviruses

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mengshi Wu ◽  
Hua Wei ◽  
Huang Tan ◽  
Shaojun Pan ◽  
Qi Liu ◽  
...  
Keyword(s):  
Dna Replication ◽  
Viral Replication ◽  
Dna Polymerase ◽  
Plant Cell ◽  
Dna Polymerases ◽  
Infected Plant ◽  
Replication Machinery ◽  
Dna Polymerase Δ ◽  
Plant Dna ◽  
Productive Replication

AbstractGeminiviruses are causal agents of devastating diseases in crops. Geminiviruses have circular single-stranded (ss) DNA genomes that are replicated in the nucleus of the infected plant cell through double-stranded (ds) DNA intermediates by the plant DNA replication machinery. Which host DNA polymerase mediates geminiviral multiplication, however, has so far remained elusive. Here, we show that subunits of the nuclear replicative DNA polymerases α and δ physically interact with the geminivirus-encoded replication enhancer protein, C3, and that these polymerases are required for viral replication. Our results suggest that, while DNA polymerase α is essential to generate the viral dsDNA intermediate, DNA polymerase δ mediates the synthesis of new copies of the geminiviral ssDNA genome, and that the virus-encoded C3 may act selectively, recruiting DNA polymerase δ over ε to favour productive replication.

scholarly journals DNA Polymerase δ Is Preferentially Recruited during Homologous Recombination To Promote Heteroduplex DNA Extension

2007 ◽  
Vol 28 (4) ◽  
pp. 1373-1382 ◽  
Author(s):  
Laurent Maloisel ◽  
Francis Fabre ◽  
Serge Gangloff
Keyword(s):  
Homologous Recombination ◽  
Gene Conversion ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Strand Break ◽  
Gene Encoding ◽  
Repair Synthesis ◽  
Dna Polymerase Δ ◽  
Strand Annealing ◽  
Gene Conversion Tract

ABSTRACT DNA polymerases play a central role during homologous recombination (HR), but the identity of the enzyme(s) implicated remains elusive. The pol3-ct allele of the gene encoding the catalytic subunit of DNA polymerase δ (Polδ) has highlighted a role for this polymerase in meiotic HR. We now address the ubiquitous role of Polδ during HR in somatic cells. We find that pol3-ct affects gene conversion tract length during mitotic recombination whether the event is initiated by single-strand gaps following UV irradiation or by site-specific double-strand breaks. We show that the pol3-ct effects on gene conversion are completely independent of mismatch repair, indicating that shorter gene conversion tracts in pol3-ct correspond to shorter extensions of primed DNA synthesis. Interestingly, we find that shorter repair tracts do not favor synthesis-dependent strand annealing at the expense of double-strand-break repair. Finally, we show that the DNA polymerases that have been previously suspected to mediate HR repair synthesis (Polε and Polη) do not affect gene conversion during induced HR, including in the pol3-ct background. Our results argue strongly for the preferential recruitment of Polδ during HR.

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