scholarly journals A Robust Actin Filaments Image Analysis Framework

2016 ◽  
Vol 12 (8) ◽  
pp. e1005063 ◽  
Author(s):  
Mitchel Alioscha-Perez ◽  
Carine Benadiba ◽  
Katty Goossens ◽  
Sandor Kasas ◽  
Giovanni Dietler ◽  
...  
Keyword(s):  
Image Analysis ◽  
Actin Filaments ◽  
Analysis Framework

scholarly journals Determination of the alpha-actinin-binding site on actin filaments by cryoelectron microscopy and image analysis.

1994 ◽  
Vol 126 (2) ◽  
pp. 433-443 ◽  
Author(s):  
A McGough ◽  
M Way ◽  
D DeRosier
Keyword(s):  
Image Analysis ◽  
Smooth Muscle ◽  
Binding Sites ◽  
Actin Filaments ◽  
Three Dimensional ◽  
Actin Binding ◽  
Dimensional Structure ◽  
Outer Face ◽  
Actin Binding Domain

The three-dimensional structure of actin filaments decorated with the actin-binding domain of chick smooth muscle alpha-actinin (alpha A1-2) has been determined to 21-A resolution. The shape and location of alpha A1-2 was determined by subtracting maps of F-actin from the reconstruction of decorated filaments. alpha A1-2 resembles a bell that measures approximately 38 A at its base and extends 42 A from its base to its tip. In decorated filaments, the base of alpha A1-2 is centered about the outer face of subdomain 2 of actin and contacts subdomain 1 of two neighboring monomers along the long-pitch (two-start) helical strands. Using the atomic model of F-actin (Lorenz, M., D. Popp, and K. C. Holmes. 1993. J. Mol. Biol. 234:826-836.), we have been able to test directly the likelihood that specific actin residues, which have been previously identified by others, interact with alpha A1-2. Our results indicate that residues 86-117 and 350-375 comprise distinct binding sites for alpha-actinin on adjacent actin monomers.

scholarly journals Brush Border Myosin–I Structure and ADP-dependent Conformational Changes Revealed by Cryoelectron Microscopy and Image Analysis

1997 ◽  
Vol 139 (3) ◽  
pp. 683-693 ◽  
Author(s):  
James D. Jontes ◽  
Ronald A. Milligan
Keyword(s):  
Image Analysis ◽  
Conformational Changes ◽  
Light Chain ◽  
Brush Border ◽  
Actin Filaments ◽  
Catalytic Domain ◽  
Binding Domain ◽  
Cryoelectron Microscopy ◽  
Skeletal Muscle Myosin ◽  
Myosin I

Brush border myosin–I (BBM-I) is a single-headed myosin found in the microvilli of intestinal epithelial cells, where it forms lateral bridges connecting the core bundle of actin filaments to the plasma membrane. Extending previous observations (Jontes, J.D., E.M. Wilson-Kubalek, and R.A. Milligan. 1995. Nature [Lond.]. 378:751–753), we have used cryoelectron microscopy and helical image analysis to generate three-dimensional (3D) maps of actin filaments decorated with BBM-I in both the presence and absence of 1 mM MgADP. In the improved 3D maps, we are able to see the entire light chain–binding domain, containing density for all three calmodulin light chains. This has enabled us to model a high resolution structure of BBM-I using the crystal structures of the chicken skeletal muscle myosin catalytic domain and essential light chain. Thus, we are able to directly measure the full magnitude of the ADP-dependent tail swing. The ∼31° swing corresponds to ∼63 Å at the end of the rigid light chain–binding domain. Comparison of the behavior of BBM-I with skeletal and smooth muscle subfragments-1 suggests that there are substantial differences in the structure and energetics of the biochemical transitions in the actomyosin ATPase cycle.

scholarly journals Image analysis framework with focus evaluation for in situ characterisation of particle size and shape attributes

2018 ◽  
Vol 191 ◽  
pp. 208-231 ◽  
Author(s):  
Javier Cardona ◽  
Carla Ferreira ◽  
John McGinty ◽  
Andrew Hamilton ◽  
Okpeafoh S. Agimelen ◽  
...  
Keyword(s):  
Particle Size ◽  
Image Analysis ◽  
Analysis Framework ◽  
Size And Shape ◽  
Particle Size And Shape

scholarly journals In‐situ 4D tomography image analysis framework to follow sintering within 3D printed glass scaffolds

2021 ◽  
Author(s):  
A. I. Kondarage ◽  
G. Poologasundarampillai ◽  
A. Nommeots‐Nomm ◽  
P.D. Lee ◽  
T.D. Lalitharatne ◽  
...  
Keyword(s):  
Image Analysis ◽  
Analysis Framework ◽  
Tomography Image ◽  
3D Printed
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