scholarly journals Cadherin-Dependent Cell Morphology in an Epithelium: Constructing a Quantitative Dynamical Model

2011 ◽  
Vol 7 (7) ◽  
pp. e1002115 ◽  
Author(s):  
Ian M. Gemp ◽  
Richard W. Carthew ◽  
Sascha Hilgenfeldt
Nanoscale ◽  
2017 ◽  
Vol 9 (35) ◽  
pp. 13171-13186 ◽  
Author(s):  
Tamaki Naganuma

Time-dependent cell morphology changed cellular capability to enhance cell adhesion force activation on nano/micro-topographies, resulting in difference cell–material interactions.


CYTOLOGIA ◽  
2012 ◽  
Vol 77 (4) ◽  
pp. 465-473 ◽  
Author(s):  
Mi^|^eacute; Ishikawa ◽  
Hironao Kataoka ◽  
Fumio Takahashi

Oncogene ◽  
1997 ◽  
Vol 15 (21) ◽  
pp. 2553-2560 ◽  
Author(s):  
Amy B Walsh ◽  
M Dhanasekaran ◽  
Dafna Bar-Sagi ◽  
C Chandra Kumar

Micromachines ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 464 ◽  
Author(s):  
Hassan Moussa ◽  
Megan Logan ◽  
Kingsley Wong ◽  
Zheng Rao ◽  
Marc Aucoin ◽  
...  

Tantalum is one of the most important biomaterials used for surgical implant devices. However, little knowledge exists about how nanoscale-textured tantalum surfaces affect cell morphology. Mammalian (Vero) cell morphology on tantalum-coated comb structures was studied using high-resolution scanning electron microscopy and fluorescence microscopy. These structures contained parallel lines and trenches with equal widths in the range of 0.18 to 100 μm. Results showed that as much as 77% of adherent cell nuclei oriented within 10° of the line axes when deposited on comb structures with widths smaller than 10 μm. However, less than 20% of cells exhibited the same alignment performance on blanket tantalum films or structures with line widths larger than 50 μm. Two types of line-width-dependent cell morphology were observed. When line widths were smaller than 0.5 μm, nanometer-scale pseudopodia bridged across trench gaps without contacting the bottom surfaces. In contrast, pseudopodia structures covered the entire trench sidewalls and the trench bottom surfaces of comb structures with line-widths larger than 0.5 μm. Furthermore, results showed that when a single cell simultaneously adhered to multiple surface structures, the portion of the cell contacting each surface reflected the type of morphology observed for cells individually contacting the surfaces.


Author(s):  
M. Marko ◽  
A. Leith ◽  
D. Parsons

The use of serial sections and computer-based 3-D reconstruction techniques affords an opportunity not only to visualize the shape and distribution of the structures being studied, but also to determine their volumes and surface areas. Up until now, this has been done using serial ultrathin sections.The serial-section approach differs from the stereo logical methods of Weibel in that it is based on the Information from a set of single, complete cells (or organelles) rather than on a random 2-dimensional sampling of a population of cells. Because of this, it can more easily provide absolute values of volume and surface area, especially for highly-complex structures. It also allows study of individual variation among the cells, and study of structures which occur only infrequently.We have developed a system for 3-D reconstruction of objects from stereo-pair electron micrographs of thick specimens.


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