scholarly journals The Genome of Deep-Sea Vent Chemolithoautotroph Thiomicrospira crunogena XCL-2

PLoS Biology ◽  
2006 ◽  
Vol 4 (12) ◽  
pp. e383 ◽  
Author(s):  
Kathleen M Scott ◽  
Stefan M Sievert ◽  
Fereniki N Abril ◽  
Lois A Ball ◽  
Chantell J Barrett ◽  
...  
Keyword(s):  
Deep Sea ◽  
Thiomicrospira Crunogena

scholarly journals Proteomic and Mutant Analysis of the CO2 Concentrating Mechanism of Hydrothermal Vent Chemolithoautotroph Thiomicrospira crunogena

2017 ◽  
Vol 199 (7) ◽  
Author(s):  
Mary Mangiapia ◽  
Terry-René W. Brown ◽  
Dale Chaput ◽  
Edward Haller ◽  
Tara L. Harmer ◽  
...  
Keyword(s):  
Deep Sea ◽  
Inorganic Carbon ◽  
Dissolved Inorganic Carbon ◽  
Carbon Uptake ◽  
Primary Input ◽  
Content Type ◽  
Transporter Family ◽  
Thiomicrospira Crunogena ◽  
Inorganic Carbon Uptake ◽  
Autotrophic Microorganisms

ABSTRACT Many autotrophic microorganisms are likely to adapt to scarcity in dissolved inorganic carbon (DIC; CO2 + HCO3 − + CO3 2−) with CO2 concentrating mechanisms (CCM) that actively transport DIC across the cell membrane to facilitate carbon fixation. Surprisingly, DIC transport has been well studied among cyanobacteria and microalgae only. The deep-sea vent gammaproteobacterial chemolithoautotroph Thiomicrospira crunogena has a low-DIC inducible CCM, though the mechanism for uptake is unclear, as homologs to cyanobacterial transporters are absent. To identify the components of this CCM, proteomes of T. crunogena cultivated under low- and high-DIC conditions were compared. Fourteen proteins, including those comprising carboxysomes, were at least 4-fold more abundant under low-DIC conditions. One of these proteins was encoded by Tcr_0854; strains carrying mutated copies of this gene, as well as the adjacent Tcr_0853, required elevated DIC for growth. Strains carrying mutated copies of Tcr_0853 and Tcr_0854 overexpressed carboxysomes and had diminished ability to accumulate intracellular DIC. Based on reverse transcription (RT)-PCR, Tcr_0853 and Tcr_0854 were cotranscribed and upregulated under low-DIC conditions. The Tcr_0853-encoded protein was predicted to have 13 transmembrane helices. Given the mutant phenotypes described above, Tcr_0853 and Tcr_0854 may encode a two-subunit DIC transporter that belongs to a previously undescribed transporter family, though it is widespread among autotrophs from multiple phyla. IMPORTANCE DIC uptake and fixation by autotrophs are the primary input of inorganic carbon into the biosphere. The mechanism for dissolved inorganic carbon uptake has been characterized only for cyanobacteria despite the importance of DIC uptake by autotrophic microorganisms from many phyla among the Bacteria and Archaea. In this work, proteins necessary for dissolved inorganic carbon utilization in the deep-sea vent chemolithoautotroph T. crunogena were identified, and two of these may be able to form a novel transporter. Homologs of these proteins are present in 14 phyla in Bacteria and also in one phylum of Archaea, the Euryarchaeota. Many organisms carrying these homologs are autotrophs, suggesting a role in facilitating dissolved inorganic carbon uptake and fixation well beyond the genus Thiomicrospira.

New data on the life history and ecology of the deep-sea hooked squid Taningia danae

Sarsia ◽  
2003 ◽  
Vol 88 (4) ◽  
pp. 297-301 ◽  
Author(s):  
Guerra A. ◽  
Rocha F. ◽  
A. F. González
Keyword(s):  
Life History ◽  
Deep Sea

Deep sea yields record-breaking bug

Nature ◽  
2006 ◽  
Author(s):  
Emma Marris
Keyword(s):  
Deep Sea

Our Deep-Sea Freighters

1920 ◽  
Vol 123 (6) ◽  
pp. 126-127
Author(s):  
Robert G. Skerrett
Keyword(s):  
Deep Sea

Deep Sea Dredgings: Examination of Sea Bottoms

1887 ◽  
Vol 24 (623supp) ◽  
pp. 9958-9958
Author(s):  
Thomas T. P. Bruce Warren
Keyword(s):  
Deep Sea
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