scholarly journals Normal microRNA Maturation and Germ-Line Stem Cell Maintenance Requires Loquacious, a Double-Stranded RNA-Binding Domain Protein

PLoS Biology ◽  
2005 ◽  
Vol 3 (7) ◽  
pp. e236 ◽  
Author(s):  
Klaus Förstemann ◽  
Yukihide Tomari ◽  
Tingting Du ◽  
Vasily V Vagin ◽  
Ahmet M Denli ◽  
...  
Keyword(s):  
Stem Cell ◽  
Rna Binding ◽  
Germ Line ◽  
Binding Domain ◽  
Double Stranded Rna ◽  
Stem Cell Maintenance ◽  
Germ Line Stem Cell ◽  
Domain Protein ◽  
Rna Binding Domain ◽  
Cell Maintenance

scholarly journals Essential requirement of mammalian Pumilio family in embryonic development

2018 ◽  
Vol 29 (24) ◽  
pp. 2922-2932 ◽  
Author(s):  
Kaibo Lin ◽  
Shikun Zhang ◽  
Qinghua Shi ◽  
Mengyi Zhu ◽  
Liuze Gao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Development ◽  
Rna Binding ◽  
Germ Line ◽  
Embryonic Stem ◽  
Cell Development ◽  
Luciferase Reporter ◽  
Essential Requirement ◽  
Stem Cell Maintenance ◽  
Cell Maintenance

Mouse PUMILIO1 (PUM1) and PUMILIO2 (PUM2) belong to the PUF (Pumilio/FBF) family, a highly conserved RNA binding protein family whose homologues play critical roles in embryonic development and germ line stem cell maintenance in invertebrates. However, their roles in mammalian embryonic development and stem cell maintenance remained largely uncharacterized. Here we report an essential requirement of the Pum gene family in early embryonic development. A loss of both Pum1 and Pum2 genes led to gastrulation failure, resulting in embryo lethality at E8.5. Pum-deficient blastocysts, however, appeared morphologically normal, from which embryonic stem cells (ESCs) could be established. Both mutant ESCs and embryos exhibited reduced growth and increased expression of endoderm markers Gata6 and Lama1, making defects in growth and differentiation the likely causes of gastrulation failure. Furthermore, ESC Gata6 transcripts could be pulled down via PUM1 immunoprecipitation and mutation of conserved PUM-binding element on 3′UTR (untranslated region) of Gata6 enhanced the expression of luciferase reporter, implicating PUM-mediated posttranscriptional regulation of Gata6 expression in stem cell development and cell lineage determination. Hence, like its invertebrate homologues, mouse PUM proteins are conserved posttranscriptional regulators essential for embryonic and stem cell development.

scholarly journals Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance

2009 ◽  
Vol 106 (23) ◽  
pp. 9304-9309 ◽  
Author(s):  
Y. Li ◽  
N. T. Minor ◽  
J. K. Park ◽  
D. M. McKearin ◽  
J. Z. Maines
Keyword(s):  
Stem Cell ◽  
Germ Line ◽  
Stem Cell Maintenance ◽  
Germ Line Stem Cell ◽  
Cell Maintenance

scholarly journals The RNA‐binding protein Imp2 regulates oxidative phosporylation that is key to glioblastoma cancer stem cell maintenance

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Michalina Janiszewska ◽  
Mario-Luca Suva ◽  
Riekelt H. Houtkooper ◽  
Virginie Clement-Schatlo ◽  
Ivan Stamenkovic
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Stem Cell Maintenance ◽  
Cell Maintenance

scholarly journals Two functionally distinct RNA-binding motifs in the regulatory domain of the protein kinase DAI.

1995 ◽  
Vol 15 (1) ◽  
pp. 358-364 ◽  
Author(s):  
S R Green ◽  
L Manche ◽  
M B Mathews
Keyword(s):  
Amino Acid ◽  
Protein Kinase ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Alpha Helix ◽  
Binding Domain ◽  
Single Amino Acid ◽  
Double Stranded Rna ◽  
C Terminus ◽  
Rna Binding Domain

The RNA-binding domain of the protein kinase DAI, the double-stranded RNA inhibitor of translation, contains two repeats of a motif that is also found in a number of other RNA-binding proteins. This motif consists of 67 amino acid residues and is predicted to contain a positively charged alpha helix at its C terminus. We have analyzed the effects of equivalent single amino acid changes in three conserved residues distributed over each copy of the motif. Mutants in the C-terminal portion of either repeat were severely defective, indicating that both copies of the motif are essential for RNA binding. Changes in the N-terminal and central parts of the motif were more debilitating if they were made in the first motif than in the second, suggesting that the first motif is the more important for RNA binding and that the second motif is structurally more flexible. When the second motif was replaced by a duplicate of the first motif, the ectopic copy retained its greater sensitivity to mutation, implying that the two motifs have distinct functions with respect to the process of RNA binding. Furthermore, the mutations have the same effect on the binding of double-stranded RNA and VA RNA, consistent with the existence of a single RNA-binding domain for both activating and inhibitory RNAs.

scholarly journals Drosophila YBX1 homolog YPS promotes ovarian germ line stem cell development by preferentially recognizing 5-methylcytosine RNAs

2020 ◽  
Vol 117 (7) ◽  
pp. 3603-3609 ◽  
Author(s):  
Fan Zou ◽  
Renjun Tu ◽  
Bo Duan ◽  
Zhenlin Yang ◽  
Zhaohua Ping ◽  
...  
Keyword(s):  
Stem Cell ◽  
Rna Binding ◽  
Germ Line ◽  
Adult Stem Cell ◽  
Cell Development ◽  
Rna Modification ◽  
Proliferation And Differentiation ◽  
Germ Line Stem Cell ◽  
Rna Complex

5-Methylcytosine (m5C) is a RNA modification that exists in tRNAs and rRNAs and was recently found in mRNAs. Although it has been suggested to regulate diverse biological functions, whether m5C RNA modification influences adult stem cell development remains undetermined. In this study, we show that Ypsilon schachtel (YPS), a homolog of human Y box binding protein 1 (YBX1), promotes germ line stem cell (GSC) maintenance, proliferation, and differentiation in the Drosophila ovary by preferentially binding to m5C-containing RNAs. YPS is genetically demonstrated to function intrinsically for GSC maintenance, proliferation, and progeny differentiation in the Drosophila ovary, and human YBX1 can functionally replace YPS to support normal GSC development. Highly conserved cold-shock domains (CSDs) of YPS and YBX1 preferentially bind to m5C RNA in vitro. Moreover, YPS also preferentially binds to m5C-containing RNAs, including mRNAs, in germ cells. The crystal structure of the YBX1 CSD-RNA complex reveals that both hydrophobic stacking and hydrogen bonds are critical for m5C binding. Overexpression of RNA-binding–defective YPS and YBX1 proteins disrupts GSC development. Taken together, our findings show that m5C RNA modification plays an important role in adult stem cell development.

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