scholarly journals Phylogenomics of the Reproductive Parasite Wolbachia pipientis wMel: A Streamlined Genome Overrun by Mobile Genetic Elements

PLoS Biology ◽  
2004 ◽  
Vol 2 (3) ◽  
pp. e69 ◽  
Author(s):  
Martin Wu ◽  
Ling V Sun ◽  
Jessica Vamathevan ◽  
Markus Riegler ◽  
Robert Deboy ◽  
...  
Keyword(s):  
Mobile Genetic Elements ◽  
Wolbachia Pipientis ◽  
Genetic Elements

scholarly journals Changes in the intralinear level of hybrid dys-genesis manifestation in Drosophila melanogaster in crosses with individuals with tetracycline suppressed internal microflora

2019 ◽  
Vol 25 ◽  
pp. 38-43
Author(s):  
I. D. Gorodnyanski
Keyword(s):  
Drosophila Melanogaster ◽  
Mobile Genetic Elements ◽  
The Body ◽  
Hybrid Dysgenesis ◽  
Control Line ◽  
Specific Interactions ◽  
Wolbachia Pipientis ◽  
Reciprocal Crosses ◽  
Genetic Elements ◽  
Reciprocal Crossing

Aim. To assess the frequency of hybrid dysgenesis in the offspring of reciprocal crosses of individuals of the Oregon-R line, with individuals of the same line, from the organism of which Wolbachia pipientis was eliminated. Methods. The line of Drosophila melanogaster Oregon R (Cambridge), in the genome of which there are MGE hobo, was investiga-ted. This line is infected with wolbachia of the wMel strain. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The study used the fifth of the generations obtained on a normal handling medium after the removal of Wolbachia. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. In the progeny from experimental crossing of females from which Wolbachia was eli-minated, with males of the control line, the proportion of females with fully reduced gonads is significantly higher than in the offspring of reciprocal crossing. Conclusions. The results obtained may be a sign of the action of the system of specific interactions of Wolbachia pipientis and Drosophila’s MGE. Keywords: mobile genetic elements, hybrid dysgenesis, Drosophila melanogaster, Wolbachia pipientis.

scholarly journals Effect of suppression of internal microflora by tetracycline on the level of manifestation of the hybrid dysgenesis of Drosophila melanogaster

2018 ◽  
Vol 22 ◽  
pp. 34-39
Author(s):  
I. D. Gorodnyanski ◽  
L. I. Vorobyova
Keyword(s):  
Drosophila Melanogaster ◽  
Mobile Genetic Elements ◽  
The Body ◽  
Hybrid Dysgenesis ◽  
Specific Interactions ◽  
Host Organism ◽  
Wolbachia Pipientis ◽  
Genetic Elements

Aim. The main goal of this article was to assess changes in the frequency of the development of hybrid dysgenesis in the Oregon-R line of Drosophila melanogaster, observed under the influence of tetracycline. Methods. The line of Drosophila melanogaster Oregon R (Cambridge), in the genome of which there are full-sized and functionally active copies of the MGE hobo, was investigated. Also, this line is infected with wolbachia of the wMel strain. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. This article presents the results of studying the effect of suppression of the internal microflora of Drosophila melanogaster – in particular, the parasitic bacterium Wolbachia pipientis - on the level of manifestation of hybrid dysgenesis. After exposure to tetracycline, a significant increase in the proportion of dysgenic individuals within the line was observed. Conclusions. The results obtained may be associated with a violation of the system of specific interactions of the host organism and internal microflora. Keywords: mobile genetic elements, hybrid dysgenesis, Drosophila melanogaster, Wolbachia pipientis.

scholarly journals Manifestation of hybrid dysgenesis in Drosophila melanogaster Harwich line with tetracycline suppressed internal microflora

2020 ◽  
Vol 26 ◽  
pp. 46-50
Author(s):  
I. D. Gorodnyanski
Keyword(s):  
Drosophila Melanogaster ◽  
Mobile Genetic Elements ◽  
The Body ◽  
Hybrid Dysgenesis ◽  
P Element ◽  
Standard Line ◽  
Wolbachia Pipientis ◽  
Genetic Elements

Aim. To evaluate the dynamics of the manifestation of hybrid dysgenesis in the Harwich line observed under the influence of tetracycline and compare the gathered data with the data for the Oregon-R line. Methods. The line of Drosophila melanogaster Harwich was investigated. This line is considered the standard line of the P cytotype. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. A stable and significant increase in the proportion of dysgenic individuals within the Harwich line was recorded after suppression of the internal microflora - including Wolbachia pipientis – by tetracycline. Conclusions. The suppression of the internal microflora of Drosophila melanogaster by tetracycline significantly affects the level of manifestation of hybrid dysgenesis caused by transpositions of the P-element. The data obtained are similar to the results of a similar study of the level of manifestation of hybrid dys-genesis caused by transpositions of hobo MGE. Keywords: mobile genetic elements, hybrid dysgenesis, P-element, Drosophila melanogaster, Wolbachia pipientis.

ISSR-PCR markers and mobile genetic elements in horse (Equus caballus) genome

2014 ◽  
pp. 42-57 ◽  
Author(s):  
N.V. Bardukov ◽  
◽  
A.V. Feofilov ◽  
T.T. Glazko ◽  
V.I. Glazko ◽  
...  
Keyword(s):  
Equus Caballus ◽  
Mobile Genetic Elements ◽  
Pcr Markers ◽  
Genetic Elements ◽  
Issr Pcr

scholarly journals Genomic evolution of antimicrobial resistance in Escherichia coli

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pimlapas Leekitcharoenphon ◽  
Markus Hans Kristofer Johansson ◽  
Patrick Munk ◽  
Burkhard Malorny ◽  
Magdalena Skarżyńska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Mobile Genetic Elements ◽  
Whole Genome ◽  
Sequencing Analysis ◽  
Metagenomic Sequencing ◽  
Fecal Samples ◽  
E Coli ◽  
Genetic Elements

AbstractThe emergence of antimicrobial resistance (AMR) is one of the biggest health threats globally. In addition, the use of antimicrobial drugs in humans and livestock is considered an important driver of antimicrobial resistance. The commensal microbiota, and especially the intestinal microbiota, has been shown to have an important role in the emergence of AMR. Mobile genetic elements (MGEs) also play a central role in facilitating the acquisition and spread of AMR genes. We isolated Escherichia coli (n = 627) from fecal samples in respectively 25 poultry, 28 swine, and 15 veal calf herds from 6 European countries to investigate the phylogeny of E. coli at country, animal host and farm levels. Furthermore, we examine the evolution of AMR in E. coli genomes including an association with virulence genes, plasmids and MGEs. We compared the abundance metrics retrieved from metagenomic sequencing and whole genome sequenced of E. coli isolates from the same fecal samples and farms. The E. coli isolates in this study indicated no clonality or clustering based on country of origin and genetic markers; AMR, and MGEs. Nonetheless, mobile genetic elements play a role in the acquisition of AMR and virulence genes. Additionally, an abundance of AMR was agreeable between metagenomic and whole genome sequencing analysis for several AMR classes in poultry fecal samples suggesting that metagenomics could be used as an indicator for surveillance of AMR in E. coli isolates and vice versa.

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