Expression of the DEAD-box/RNA Helicase rck/p54 in Mouse Tissues: Implications for Heterogeneous Protein Expression

2006 ◽  
Vol 54 (8) ◽  
pp. 955-960 ◽  
Author(s):  
Yukihiro Akao ◽  
Yoshihito Nakagawa
Keyword(s):  
Protein Expression ◽  
Rna Helicase ◽  
Dead Box ◽  
Mouse Tissues ◽  
The Dead

scholarly journals Association of the Cold Shock DEAD-Box RNA Helicase RhlE to the RNA Degradosome in Caulobacter crescentus

2017 ◽  
Vol 199 (13) ◽  
Author(s):  
Angel A. Aguirre ◽  
Alexandre M. Vicente ◽  
Steven W. Hardwick ◽  
Daniela M. Alvelos ◽  
Ricardo R. Mazzon ◽  
...  
Keyword(s):  
Low Temperature ◽  
Caulobacter Crescentus ◽  
Immunoblot Analysis ◽  
Rna Helicase ◽  
Rnase E ◽  
Rna Helicases ◽  
Content Type ◽  
Dead Box ◽  
Rna Degradosome ◽  
The Dead

ABSTRACT In diverse bacterial lineages, multienzyme assemblies have evolved that are central elements of RNA metabolism and RNA-mediated regulation. The aquatic Gram-negative bacterium Caulobacter crescentus, which has been a model system for studying the bacterial cell cycle, has an RNA degradosome assembly that is formed by the endoribonuclease RNase E and includes the DEAD-box RNA helicase RhlB. Immunoprecipitations of extracts from cells expressing an epitope-tagged RNase E reveal that RhlE, another member of the DEAD-box helicase family, associates with the degradosome at temperatures below those optimum for growth. Phenotype analyses of rhlE, rhlB, and rhlE rhlB mutant strains show that RhlE is important for cell fitness at low temperature and its role may not be substituted by RhlB. Transcriptional and translational fusions of rhlE to the lacZ reporter gene and immunoblot analysis of an epitope-tagged RhlE indicate that its expression is induced upon temperature decrease, mainly through posttranscriptional regulation. RNase E pulldown assays show that other proteins, including the transcription termination factor Rho, a second DEAD-box RNA helicase, and ribosomal protein S1, also associate with the degradosome at low temperature. The results suggest that the RNA degradosome assembly can be remodeled with environmental change to alter its repertoire of helicases and other accessory proteins. IMPORTANCE DEAD-box RNA helicases are often present in the RNA degradosome complex, helping unwind secondary structures to facilitate degradation. Caulobacter crescentus is an interesting organism to investigate degradosome remodeling with change in temperature, because it thrives in freshwater bodies and withstands low temperature. In this study, we show that at low temperature, the cold-induced DEAD-box RNA helicase RhlE is recruited to the RNA degradosome, along with other helicases and the Rho protein. RhlE is essential for bacterial fitness at low temperature, and its function may not be complemented by RhlB, although RhlE is able to complement for rhlB loss. These results suggest that RhlE has a specific role in the degradosome at low temperature, potentially improving adaptation to this condition.

scholarly journals The embryonic RNA helicase gene (ERH): a new member of the DEAD box family of RNA helicases

1995 ◽  
Vol 308 (3) ◽  
pp. 839-846 ◽  
Author(s):  
J Sowden ◽  
W Putt ◽  
K Morrison ◽  
R Beddington ◽  
Y Edwards
Keyword(s):  
Expression Profile ◽  
Sequence Similarity ◽  
Rna Helicase ◽  
Chromosome 1 ◽  
Rna Helicases ◽  
High Sequence Similarity ◽  
Dead Box ◽  
Isolation And Characterization ◽  
Helicase Gene ◽  
The Dead

DEAD box proteins share several highly conserved motifs including the characteristic Asp-Glu-Ala-Asp (D-E-A-D in the amino acid single-letter code) motif and have established or putative ATP-dependent RNA helicase activity. These proteins are implicated in a range of cellular processes that involve regulation of RNA function, including translation initiation, RNA splicing and ribosome assembly. Here we describe the isolation and characterization of an embryonic RNA helicase gene, ERH, which maps to mouse chromosome 1 and encodes a new member of the DEAD box family of proteins. The predicted ERH protein shows high sequence similarity to the testes-specific mouse PL10 and to the maternally acting Xenopus An3 helicase proteins. The ERH expression profile is similar, to that of An3, which localizes to the animal hemisphere of oocytes and is abundantly expressed in the embryo. ERH is expressed in oocytes and is a ubiquitous mRNA in the 9 days-post-conception embryo, and at later stages of development shows a more restricted pattern of expression in brain and kidney. The similarities in sequence and in expression profile suggest that ERH is the murine equivalent of the Xenopus An3 gene, and we propose that ERH plays a role in translational activation of mRNA in the oocyte and early embryo.

scholarly journals The DEAD-box RNA helicase Vad1 regulates multiple virulence-associated genes in Cryptococcus neoformans

2005 ◽  
Vol 115 (3) ◽  
pp. 632-641 ◽  
Author(s):  
John Panepinto ◽  
Lide Liu ◽  
Jeanie Ramos ◽  
Xudong Zhu ◽  
Tibor Valyi-Nagy ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Rna Helicase ◽  
Dead Box ◽  
The Dead

Hydrodynamic Characterization of the DEAD-box RNA Helicase DbpA

2006 ◽  
Vol 355 (4) ◽  
pp. 697-707 ◽  
Author(s):  
Miguel A. Talavera ◽  
Erin E. Matthews ◽  
William K. Eliason ◽  
Irit Sagi ◽  
Jimin Wang ◽  
...  
Keyword(s):  
Rna Helicase ◽  
Dead Box ◽  
The Dead

scholarly journals Assembly and Dynamics of Liquid Phase Protein Droplets Comprised of the DEAD-Box RNA Helicase, LAF-1

2015 ◽  
Vol 108 (2) ◽  
pp. 230a-231a
Author(s):  
Shana Elbaum ◽  
Younghoon Kim ◽  
Sua Myong ◽  
Clifford Brangwynne
Keyword(s):  
Liquid Phase ◽  
Rna Helicase ◽  
Dead Box ◽  
Phase Protein ◽  
The Dead

Signaling to the DEAD box—Regulation of DEAD-box p68 RNA helicase by protein phosphorylations

2005 ◽  
Vol 17 (12) ◽  
pp. 1495-1504 ◽  
Author(s):  
Liuqing Yang ◽  
Chunru Lin ◽  
Zhi-Ren Liu
Keyword(s):  
Rna Helicase ◽  
Dead Box ◽  
P68 Rna Helicase ◽  
The Dead

scholarly journals The DEAD-Box RNA Helicase AtRH7/PRH75 Participates in Pre-rRNA Processing, Plant Development and Cold Tolerance in Arabidopsis

2015 ◽  
Vol 57 (1) ◽  
pp. 174-191 ◽  
Author(s):  
Chun-Kai Huang ◽  
Yu-Lien Shen ◽  
Li-Fen Huang ◽  
Shaw-Jye Wu ◽  
Chin-Hui Yeh ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Plant Development ◽  
Rna Helicase ◽  
Processing Plant ◽  
Rrna Processing ◽  
Dead Box ◽  
The Dead
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