scholarly journals On the Difference Between Surface-Enhanced Raman Scattering (SERS) Spectra of Cell Growth Media and Whole Bacterial Cells

2011 ◽  
Vol 65 (5) ◽  
pp. 493-499 ◽  
Author(s):  
W. Ranjith Premasiri ◽  
Yoseph Gebregziabher ◽  
Lawrence D. Ziegler
Keyword(s):  
Cell Growth ◽  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Growth Media ◽  
Bacterial Cells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
The Difference

scholarly journals Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Mustafa Çulha ◽  
M. Müge Yazıcı ◽  
Mehmet Kahraman ◽  
Fikrettin Şahin ◽  
Sesin Kocagöz
Keyword(s):  
Silver Nanoparticles ◽  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Growth Media ◽  
Bacterial Cells ◽  
Convective Assembly ◽  
Assembly Method ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Whole bacterial cell characterization is critically important for fast bacterial identification. Surface-enhanced Raman scattering (SERS) is proven to be powerful technique to serve such a goal. In this study, the characterization of whole bacterial cells in the microwells constructed from colloidal silver nanoparticles (AgNPs) with “convective-assembly” method is reported. The proper size of the microwells for the model bacteria,Escherichia coliandStaphylococcus cohnii, is determined to be 1.2 μm from their electron microscopy images. A minimum dilution factor of 20 is necessary for the bacterial samples collected from growth media to diminish the bacterial aggregation to place the bacterial cells into the microwells. The constructed microwell structures are tested for their bacterial SERS performance and compared to the SERS spectra obtained from the samples prepared with a simple mixing of bacteria and AgNPs for the same bacteria. The results indicate that microwell structures not only improve the spectral quality but also increase the reproducibility of the SERS spectra.

scholarly journals Peptide-Guided Surface-Enhanced Raman Scattering Probes for Localized Cell Composition Analysis

2012 ◽  
Vol 78 (21) ◽  
pp. 7805-7808 ◽  
Author(s):  
Ahmad I. M. Athamneh ◽  
Ryan S. Senger
Keyword(s):  
Raman Scattering ◽  
Analytical Techniques ◽  
Surface Enhanced Raman Scattering ◽  
Composition Analysis ◽  
Bacterial Cells ◽  
Cell Composition ◽  
Hydrophobic Peptides ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

ABSTRACTThe ability to control the localization of surface-enhanced Raman scattering (SERS) nanoparticle probes in bacterial cells is critical to the development of analytical techniques that can nondestructively determine cell composition and phenotype. Here, selective localization of SERS probes was achieved at the outer bacterial membrane by using silver nanoparticles functionalized with synthetic hydrophobic peptides.

scholarly journals Portable bacteria-capturing chip for direct surface-enhanced Raman scattering identification of urinary tract infection pathogens

2018 ◽  
Vol 5 (9) ◽  
pp. 180955 ◽  
Author(s):  
Danting Yang ◽  
Haibo Zhou ◽  
Nicoleta E. Dina ◽  
Christoph Haisch
Keyword(s):  
Urinary Tract ◽  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Clinical Diagnostics ◽  
Detection Methods ◽  
Bacterial Cells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Tract Infections

Acute urinary tract infections (UTIs) are one of the most common nosocomial bacterial infections, which affect almost 50% of the population at least once in their lifetime. UTIs may lead to lethal consequences if they are left undiagnosed and not properly treated. Early, rapid and accurate uropathogens detection methods play a pivotal role in clinical process. In this work, a portable bacteria-grasping surface-enhanced Raman scattering (SERS) chip for identification of three species of uropathogens ( Escherichia coli CFT 073, Pseudomonas aeruginosa PAO1 and Proteus mirabilis PRM1) directly from culture matrix was reported. The chip was firstly modified with a positively charged NH 3 + group, which enables itself grasp the negatively charged bacterial cells through the electrostatic adsorption principle. After the bacterial cells were captured by the chip, concentrated Ag nanoparticles (NPs) were used to obtain their Raman fingerprint spectra with recognizable characteristic peaks and good reproducibility. With the help of chemometric method such as discriminant analysis (DA), the SERS-based chip allows a rapid, successful identification of three species of UTI bacteria with a minimal bacterial concentration (10 5 cells ml −1 ) required for clinical diagnostics. In addition, this chip could spot the bacterial SERS fingerprints information directly from LB culture medium and artificial urine without sample pre-treatment. The portable bacteria-grasping SERS-based chip provides a possibility for fast and easy detection of uropathogens, and viability of future development in healthcare applications.

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