Non-Resonant 2 Color 2-Dimensional Electronic Spectroscopy Reveals Ground State Coherences of Light Harvesting Complex II

2020 ◽  
Author(s):  
Shawn Irgen-Gioro ◽  
Elad Harel
Keyword(s):  
Ground State ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

scholarly journals Observation of dissipative chlorophyll-to-carotenoid energy transfer in light-harvesting complex II in membrane nanodiscs

2019 ◽  
Author(s):  
Minjung Son ◽  
Alberta Pinnola ◽  
Samuel C. Gordon ◽  
Roberto Bassi ◽  
Gabriela S. Schlau-Cohen
Keyword(s):  
Energy Transfer ◽  
Conformational Changes ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Local Environment ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii ◽  
Photosynthetic Antenna

<pre><p><a></a>Green plants prevent photodamage under high light conditions by dissipating excess energy as heat. Conformational changes of the photosynthetic antenna complexes activate dissipation by leveraging the sensitivity of the photophysics of the chlorophyll and carotenoids to their surrounding protein. However, the mechanisms and site of dissipation are still debated, largely due to two challenges. First, because of the ultrafast timescales and large energy gaps involved, measurements lacked the temporal or spectral requirements. Second, experiments have been performed in detergent, which can induce non-native conformations, or <i>in vivo</i>, where contributions from the multiple complexes cannot be disentangled and are further obfuscated by laser-induced artifacts. Here, we overcome both challenges by applying ultrabroadband two-dimensional electronic spectroscopy to the principal antenna complex, light-harvesting complex II, in a near-native membrane. The membrane enhances two dissipative pathways, one of which was previously uncharacterized chlorophyll-to-carotenoid energy transfer. Our results highlight the sensitivity of the photophysics to the local environment, which may be used to control the balance between light harvesting and dissipation <i>in vivo</i>.</p></pre>

scholarly journals Pathways of energy transfer in LHCII revealed by room-temperature 2D electronic spectroscopy

2014 ◽  
Vol 16 (23) ◽  
pp. 11640-11646 ◽  
Author(s):  
Kym L. Wells ◽  
Petar H. Lambrev ◽  
Zhengyang Zhang ◽  
Gyözö Garab ◽  
Howe-Siang Tan
Keyword(s):  
Energy Transfer ◽  
Room Temperature ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Spectroscopy Study ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

We present here the first room-temperature 2D electronic spectroscopy study of energy transfer in the plant light-harvesting complex II, LHCII.

scholarly journals Two-Dimensional Electronic Spectroscopy of Light-Harvesting Complex II at Ambient Temperature: A Joint Experimental and Theoretical Study

2015 ◽  
Vol 119 (36) ◽  
pp. 12017-12027 ◽  
Author(s):  
Hong-Guang Duan ◽  
Amy L. Stevens ◽  
Peter Nalbach ◽  
Michael Thorwart ◽  
Valentyn I. Prokhorenko ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Theoretical Study ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Two Dimensional ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

scholarly journals Observation of dissipative chlorophyll-to-carotenoid energy transfer in light-harvesting complex II in membrane nanodiscs

2019 ◽  
Author(s):  
Minjung Son ◽  
Alberta Pinnola ◽  
Samuel C. Gordon ◽  
Roberto Bassi ◽  
Gabriela S. Schlau-Cohen
Keyword(s):  
Energy Transfer ◽  
Conformational Changes ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Local Environment ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii ◽  
Photosynthetic Antenna

<pre><p><a></a>Green plants prevent photodamage under high light conditions by dissipating excess energy as heat. Conformational changes of the photosynthetic antenna complexes activate dissipation by leveraging the sensitivity of the photophysics of the chlorophyll and carotenoids to their surrounding protein. However, the mechanisms and site of dissipation are still debated, largely due to two challenges. First, because of the ultrafast timescales and large energy gaps involved, measurements lacked the temporal or spectral requirements. Second, experiments have been performed in detergent, which can induce non-native conformations, or <i>in vivo</i>, where contributions from the multiple complexes cannot be disentangled and are further obfuscated by laser-induced artifacts. Here, we overcome both challenges by applying ultrabroadband two-dimensional electronic spectroscopy to the principal antenna complex, light-harvesting complex II, in a near-native membrane. The membrane enhances two dissipative pathways, one of which was previously uncharacterized chlorophyll-to-carotenoid energy transfer. Our results highlight the sensitivity of the photophysics to the local environment, which may be used to control the balance between light harvesting and dissipation <i>in vivo</i>.</p></pre>

Dissipative Pathways in Light-Harvesting Complex II Are Controlled by the Plant Membrane

2019 ◽  
Author(s):  
Minjung Son ◽  
Alberta Pinnola ◽  
Samuel C. Gordon ◽  
Roberto Bassi ◽  
Gabriela S. Schlau-Cohen
Keyword(s):  
Conformational Changes ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Light Conditions ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Energy Gaps ◽  
Light Harvesting Complex Ii ◽  
Photosynthetic Antenna

<p>Green plants prevent photodamage under high light conditions by dissipating excess energy as heat. Conformational changes of the photosynthetic antenna complexes activate dissipation by leveraging the sensitivity of the photophysics of the chlorophyll and carotenoids to their surrounding protein. However, the mechanisms and site of dissipation are still debated, largely due to two challenges. First, experiments have been performed in detergent, which can induce non-native conformations, or <i>in vivo</i>, where contributions from the multiple complexes cannot be disentangled and are further obfuscated by laser-induced artifacts. Second, because of the ultrafast timescales and large energy gaps involved, measurements lacked the temporal or spectral requirements. Here, we overcome both challenges by applying ultrabroadband two-dimensional electronic spectroscopy to the principal antenna complex, light-harvesting complex II, in a near-native membrane. The spectra show that the membrane enhances two dissipative pathways, one of which was hypothesized yet previously uncharacterized. Our results suggest a resting level of dissipation that may protect against sudden solar fluctuations, and highlight that this level can even be fine-tuned by the membrane environment.</p>

scholarly journals Energy transfer dynamics in trimers and aggregates of light-harvesting complex II probed by 2D electronic spectroscopy

2015 ◽  
Vol 142 (21) ◽  
pp. 212432 ◽  
Author(s):  
Miriam M. Enriquez ◽  
Parveen Akhtar ◽  
Cheng Zhang ◽  
Győző Garab ◽  
Petar H. Lambrev ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

Macroorganisation and flexibility of thylakoid membranes

2019 ◽  
Vol 476 (20) ◽  
pp. 2981-3018 ◽  
Author(s):  
Petar H. Lambrev ◽  
Parveen Akhtar
Keyword(s):  
Light Harvesting ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Photosynthetic Apparatus ◽  
Dynamic Responses ◽  
State Transitions ◽  
Photochemical Quenching ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

Abstract The light reactions of photosynthesis are hosted and regulated by the chloroplast thylakoid membrane (TM) — the central structural component of the photosynthetic apparatus of plants and algae. The two-dimensional and three-dimensional arrangement of the lipid–protein assemblies, aka macroorganisation, and its dynamic responses to the fluctuating physiological environment, aka flexibility, are the subject of this review. An emphasis is given on the information obtainable by spectroscopic approaches, especially circular dichroism (CD). We briefly summarise the current knowledge of the composition and three-dimensional architecture of the granal TMs in plants and the supramolecular organisation of Photosystem II and light-harvesting complex II therein. We next acquaint the non-specialist reader with the fundamentals of CD spectroscopy, recent advances such as anisotropic CD, and applications for studying the structure and macroorganisation of photosynthetic complexes and membranes. Special attention is given to the structural and functional flexibility of light-harvesting complex II in vitro as revealed by CD and fluorescence spectroscopy. We give an account of the dynamic changes in membrane macroorganisation associated with the light-adaptation of the photosynthetic apparatus and the regulation of the excitation energy flow by state transitions and non-photochemical quenching.

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