scholarly journals Total Internal Reflection Tomography (TIRT) for Three-Dimensional Sub-Wavelength Imaging

2001 ◽  
Author(s):  
David G. Fischer ◽  
P. Scott Carney ◽  
John C. Schotland
Keyword(s):  
Total Internal Reflection ◽  
Three Dimensional ◽  
Internal Reflection ◽  
Reflection Tomography ◽  
Sub Wavelength

Tracking of Nanoparticles Using Evanescent Wave Microscopy

Volume 4 ◽  
2004 ◽  
Author(s):  
Arindam Banerjee ◽  
Kenneth D. Kihm
Keyword(s):  
Diffusion Coefficient ◽  
Evanescent Wave ◽  
Total Internal Reflection ◽  
Tracer Particle ◽  
Three Dimensional ◽  
Internal Reflection ◽  
Brownian Diffusion ◽  
Wall Region ◽  
Identification Algorithm ◽  
Polystyrene Spheres

A novel three-dimensional tracking technique of nanoparticles in nanometer spatial resolution using a Ratiometric Total Internal Reflection Fluorescence Microscopy (R-TIRFM) is presented. Evanescent waves from the total internal reflection of an argon-ion laser (488 nm) are used to generate a thin sliced illumination field with its effective visualization range of 544-nm, equivalent to twice of the penetration depth of the evanescent wave field. Fluorescence-coated polystyrene spheres of 500-nm diameter (SG = 1.05) are used as tracers subjected to the Brownian diffusive motions. A ratiometric analysis of the fluorescence particle images together with a neural network particle-pair identification algorithm is used to track the tracer particle locations across multiple image frames in full three-dimensional ways. The techniques are used to examine the Brownian diffusive motion of nanoparticles as they approach the very near-wall region within a few hundred nanometers of a glass-water interface. The experimental results show that the measured Brownian diffusion coefficient is in good agreement with the theoretical hindered diffusion coefficient near a wall.

Superresolution in total internal reflection tomography

2005 ◽  
Vol 22 (9) ◽  
pp. 1889 ◽  
Author(s):  
Kamal Belkebir ◽  
Patrick C. Chaumet ◽  
Anne Sentenac
Keyword(s):  
Total Internal Reflection ◽  
Internal Reflection ◽  
Reflection Tomography

Direct Measurement of Slip Velocities Using Three-Dimensional Total Internal Reflection Velocimetry

2005 ◽  
Author(s):  
Peter Huang ◽  
Jeffrey Guasto ◽  
Kenneth Breuer
Keyword(s):  
Shear Rate ◽  
Total Internal Reflection ◽  
Three Dimensional ◽  
Surface Hydrophobicity ◽  
Internal Reflection ◽  
Boundary Slip ◽  
Shear Rates ◽  
Rate Dependent ◽  
Close Proximity ◽  
Low Shear

The possible existence of slip of liquids in close proximity to a smooth surface is studied experimentally via the dynamics of small particles suspended in a shear flow. Sub-micron fluorescent particles suspended in water are imaged and analyzed using Total Internal Reflection Velocimetry (TIRV). For water flowing over a hydrophilic surface, the measurements are in agreement with previous experiments and indicate that slip, if present, is minimal at low shear rates, but increases slightly as the shear rate increases. Furthermore, surface hydrophobicity can be attributed for additional shear-rate dependent boundary slip. Issues associated with the experimental technique and the interpretation of results are also discussed.

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