scholarly journals High-resolution two-photon transcranial imaging of brain using direct wavefront sensing

2021 ◽  
Author(s):  
Congping Chen ◽  
Zhongya Qin ◽  
Sicong He ◽  
Shaojun Liu ◽  
Shun-Fat Lau ◽  
...  
Keyword(s):  
High Resolution ◽  
Wavefront Sensing ◽  
Two Photon ◽  
Transcranial Imaging

scholarly journals High-resolution two-photon transcranial imaging of brain using direct wavefront sensing

2020 ◽  
Author(s):  
Congping Chen ◽  
Zhongya Qin ◽  
Sicong He ◽  
Shaojun Liu ◽  
Shun-Fat Lau ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
Pyramidal Neurons ◽  
Imaging System ◽  
Superficial Layer ◽  
Wavefront Sensing ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Transcranial Imaging

AbstractImaging of the brain in its native state at high resolution poses major challenges to visualization techniques. Two-photon microscopy integrated with the thinned-skull or optical clearing skull technique provides a minimally invasive tool for in vivo imaging of the cortex of mice without activating immune response and inducing brain injury. However, the imaging contrast and resolution are severely compromised by the optical heterogeneity of the skull, limiting the imaging depth to the superficial layer. Here, we develop adaptive optics two-photon microscopy for high-resolution transcranial imaging of layer 5 pyramidal neurons up to 700 μm below pia in living mice. In particular, an optimized configuration of imaging system and new wavefront sensing algorithm are proposed for accurate correction for the aberrations induced by the skull window and brain tissue. We investigated microglia-plaque interaction in living brain of Alzheimer’s disease and demonstrated high-precision laser dendrotomy and single-spine ablation.

scholarly journals High-resolution spectral characterization of two photon states via classical measurements

2014 ◽  
Vol 8 (5) ◽  
pp. L76-L80 ◽  
Author(s):  
Andreas Eckstein ◽  
Guillaume Boucher ◽  
Aristide Lemaître ◽  
Pascal Filloux ◽  
Ivan Favero ◽  
...  
Keyword(s):  
High Resolution ◽  
Spectral Characterization ◽  
Two Photon ◽  
Photon States

scholarly journals The Power of Single and Multibeam Two-Photon Microscopy for High-Resolution and High-Speed Deep Tissue and Intravital Imaging

2007 ◽  
Vol 93 (7) ◽  
pp. 2519-2529 ◽  
Author(s):  
Raluca Niesner ◽  
Volker Andresen ◽  
Jens Neumann ◽  
Heinrich Spiecker ◽  
Matthias Gunzer
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Intravital Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

High-resolution two-photon spectroscopy in InSb at milliwatt cw powers in a magnetic field

1981 ◽  
Vol 23 (12) ◽  
pp. 6806-6809 ◽  
Author(s):  
D. G. Seiler ◽  
M. W. Goodwin ◽  
M. H. Weiler
Keyword(s):  
Magnetic Field ◽  
High Resolution ◽  
Two Photon

scholarly journals Absolute frequency of cesium 6S–8S 822 nm two-photon transition by a high-resolution scheme

2013 ◽  
Vol 38 (16) ◽  
pp. 3186 ◽  
Author(s):  
Chien-Ming Wu ◽  
Tze-Wei Liu ◽  
Ming-Hsuan Wu ◽  
Ray-Kuang Lee ◽  
Wang-Yau Cheng
Keyword(s):  
High Resolution ◽  
Absolute Frequency ◽  
Two Photon ◽  
Photon Transition ◽  
Resolution Scheme
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