scholarly journals High-Resolution Fourier Light-Field Microscopy for Volumetric Multi-Color Live-Cell Imaging

Optica ◽  
2021 ◽  
Author(s):  
Shu Jia ◽  
Xuanwen Hua ◽  
Wenhao Liu
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Light Field ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Fast, volumetric live-cell imaging using high-resolution light-field microscopy

2019 ◽  
Vol 10 (1) ◽  
pp. 29 ◽  
Author(s):  
Haoyu Li ◽  
Changliang Guo ◽  
Deborah Kim-Holzapfel ◽  
Weiyi Li ◽  
Yelena Altshuller ◽  
...  
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Light Field ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Fast, volumetric live-cell imaging using high-resolution light-field microscopy

2018 ◽  
Author(s):  
Haoyu Li ◽  
Changliang Guo ◽  
Deborah Kim-Holzapfel ◽  
Weiyi Li ◽  
Yelena Altshuller ◽  
...  
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Light Field ◽  
Cell Imaging ◽  
Spatial Scales ◽  
Functional Brain Imaging ◽  
Live Cell ◽  
Three Dimensions ◽  
Acquisition Time ◽  
Spatio Temporal

AbstractVisualizing diverse anatomical and functional traits that span many spatial scales with high spatio-temporal resolution provides insights into the fundamentals of living organisms. Light-field microscopy (LFM) has recently emerged as a scanning-free, scalable method that allows for high-speed, volumetric functional brain imaging. Given those promising applications at the tissue level, at its other extreme, this highly-scalable approach holds great potential for observing structures and dynamics in single-cell specimens. However, the challenge remains for current LFM to achieve subcellular level, near-diffraction-limited 3D spatial resolution. Here, we report high-resolution LFM (HR-LFM) for live-cell imaging with a resolution of 300-700 nm in all three dimensions, an imaging depth of several micrometers, and a volume acquisition time of milliseconds. We demonstrate the technique by imaging various cellular dynamics and structures and tracking single particles. The method may advance LFM as a particularly useful tool for understanding biological systems at multiple spatio-temporal levels.

Fast microfluidic temperature control for high resolution live cell imaging

Lab on a Chip ◽  
2011 ◽  
Vol 11 (3) ◽  
pp. 484-489 ◽  
Author(s):  
Guilhem Velve Casquillas ◽  
Chuanhai Fu ◽  
Mael Le Berre ◽  
Jeremy Cramer ◽  
Sebastien Meance ◽  
...  
Keyword(s):  
High Resolution ◽  
Temperature Control ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals A Stretching Device for High-Resolution Live-Cell Imaging

2010 ◽  
Vol 38 (5) ◽  
pp. 1728-1740 ◽  
Author(s):  
Lawrence Huang ◽  
Pattie S. Mathieu ◽  
Brian P. Helmke
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals High-resolution live-cell imaging reveals novel cyclin A2 degradation foci involving autophagy

2014 ◽  
Vol 127 (10) ◽  
pp. 2145-2150 ◽  
Author(s):  
Abdelhalim Loukil ◽  
Manuela Zonca ◽  
Cosette Rebouissou ◽  
Véronique Baldin ◽  
Olivier Coux ◽  
...  
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cyclin A2

scholarly journals Optical diffraction tomography for high resolution live cell imaging

2009 ◽  
Vol 17 (1) ◽  
pp. 266 ◽  
Author(s):  
Yongjin Sung ◽  
Wonshik Choi ◽  
Christopher Fang-Yen ◽  
Kamran Badizadegan ◽  
Ramachandra R. Dasari ◽  
...  
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography
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