Exploiting the potential of commercial objectives to extend the field-of-view of two-photon microscopy by adaptive optics

2022 ◽  
Author(s):  
Jing Yao ◽  
Yufeng Gao ◽  
Yixuan Yin ◽  
Shiwei Ye ◽  
Wei Zheng
Keyword(s):  
Adaptive Optics ◽  
Field Of View ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Publisher Correction: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

2021 ◽  
Vol 18 (2) ◽  
pp. 220-220
Author(s):  
Weijian Zong ◽  
Runlong Wu ◽  
Shiyuan Chen ◽  
Junjie Wu ◽  
Hanbin Wang ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Field Of View ◽  
Two Photon Microscopy ◽  
Two Photon

Intravital multiphoton microscopy of dynamic renal processes

2005 ◽  
Vol 288 (6) ◽  
pp. F1084-F1089 ◽  
Author(s):  
Bruce A. Molitoris ◽  
Ruben M. Sandoval
Keyword(s):  
Proximal Tubule ◽  
Multiphoton Microscopy ◽  
Field Of View ◽  
Glomerular Permeability ◽  
Structural Alterations ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Dynamic Events ◽  
Subcellular Resolution ◽  
Functioning Kidney

Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within the same field of view over seconds to weeks. This approach invigorates the validity of data and facilitates analysis and interpretation as trends are more readily determined when one is more closely monitoring indicative physiological parameters. Therefore, in this review we emphasize how specific approaches will enable studies into glomerular permeability, proximal tubule endocytosis, and microvascular function within the kidney. We attempt to show how visual data can be quantified, thus allowing enhanced understanding of the process under study. Finally, emphasis is given to the possible future opportunities of this technology and its present limitations.

scholarly journals Multidither coherent optical adaptive technique for deep tissue two-photon microscopy

2019 ◽  
Vol 12 (04) ◽  
pp. 1942003 ◽  
Author(s):  
Biwei Zhang ◽  
Wei Gong ◽  
Chenxue Wu ◽  
Lejia Hu ◽  
Xinpei Zhu ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Diffraction Theory ◽  
Fluorescence Signal ◽  
Two Photon Microscopy ◽  
Scalar Diffraction ◽  
Two Photon ◽  
Scalar Diffraction Theory ◽  
Adaptive Technique ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging. Multidither coherent optical adaptive technique (COAT) can correct the scattered wavefront in parallel. However, the determination of the corrective phases may not be completely accurate using conventional method, which undermines the performance of this technique. In this paper, we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fluorescence signal. A numerical simulation model is established to study the performance of adaptive optics in two-photon microscopy by combining scalar diffraction theory with vector diffraction theory. The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging. In our simulation, with the scattering from a 450-[Formula: see text]m-thick mouse brain tissue, excitation focal spots with higher peak-to-background ratio (PBR) and images with higher contrast can be obtained. Hence, further enhancement of the multidither COAT correction performance in two-photon imaging can be expected.

scholarly journals Axial resolution improvement of two-photon microscopy by multi-frame reconstruction and adaptive optics

2020 ◽  
Vol 11 (11) ◽  
pp. 6634
Author(s):  
Shiwei Ye ◽  
Yixuan Yin ◽  
Jing Yao ◽  
Jun Nie ◽  
Yuchen Song ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Axial Resolution ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Resolution Improvement

Aberration-free 3D imaging via DMD-based two-photon microscopy and sensorless adaptive optics

2020 ◽  
Vol 45 (9) ◽  
pp. 2656 ◽  
Author(s):  
Mindan Ren ◽  
Jialong Chen ◽  
Dihan Chen ◽  
Shih-Chi Chen
Keyword(s):  
Adaptive Optics ◽  
3D Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Zhongya Qin ◽  
Sicong He ◽  
Chao Yang ◽  
Jasmine Sum-Yee Yung ◽  
Congping Chen ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Retinal Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view

2008 ◽  
Vol 16 (14) ◽  
pp. 10066 ◽  
Author(s):  
Y. Kremer ◽  
J.-F. Léger ◽  
R. Lapole ◽  
N. Honnorat ◽  
Y. Candela ◽  
...  
Keyword(s):  
Field Of View ◽  
Large Field ◽  
Two Photon Microscopy ◽  
Two Photon

Pulse front adaptive optics in two-photon microscopy

2015 ◽  
Vol 40 (21) ◽  
pp. 4999 ◽  
Author(s):  
Bangshan Sun ◽  
Patrick S. Salter ◽  
Martin J. Booth
Keyword(s):  
Adaptive Optics ◽  
Pulse Front ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry

2020 ◽  
Author(s):  
Che-Hang Yu ◽  
Jeffrey N. Stirman ◽  
Yiyi Yu ◽  
Riichiro Hira ◽  
Spencer L. Smith
Keyword(s):  
Adaptive Optics ◽  
Calcium Imaging ◽  
High Speed ◽  
Optical Design ◽  
Neural Circuitry ◽  
Brain Regions ◽  
Field Of View ◽  
Large Field ◽  
Two Photon ◽  
Subcellular Resolution

AbstractImaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large Field-of-view Two-Photon imaging (Diesel2p). Combining novel optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view (∼ 25 mm2) with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.

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