Real-time capture of single-particles in controlled flow by rapidly generated foci-array with adjustable intensity and pattern

2021 ◽  
Author(s):  
Chenchu Zhang ◽  
Hanchang Ye ◽  
Chaowei Wang ◽  
Jingjing Zhang ◽  
Linhan Zhao ◽  
...  
Keyword(s):  
Real Time ◽  
Single Particles ◽  
Controlled Flow

Electrostatic Sensor for Real–Time Mass Flow Rate Measurement of Particle Conveying in Pneumatic Pipeline

2012 ◽  
Author(s):  
Mohd. Fua’ad Rahmat ◽  
Wee Lee Yaw
Keyword(s):  
Real Time ◽  
Mass Flow Rate ◽  
Mass Flow ◽  
Flow Rate ◽  
Cross Correlation ◽  
High Accuracy ◽  
Industrial Processes ◽  
Rate Measurement ◽  
Flow Rate Measurement ◽  
Controlled Flow

This paper discussed the electrostatic sensors that have been constructed for real–time mass flow rate measurement of particle conveying in a Pneumatic pipeline. Many industrial processes require continuous, smooth, and consistent delivery of solids materials with a high accuracy of controlled flow rate. This requirement can only be achieved by installing a proper measurement system. Electrostatic sensor offers the most inexpensive and simplest means of measuring solids flows in pipes. Key words: Electrostatic sensor, cross-correlation, peripheral velocity

scholarly journals Integration of Microresonant Sensor into a Microfluidic Platform for the Real Time Analysis of Platelets-Collagen Interaction in Flow Condition

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 940
Author(s):  
Aleksandr Oseev ◽  
Benoît Le Roy de Boiseaumarié ◽  
Fabien Remy-Martin ◽  
Jean-François Manceau ◽  
Alain Rouleau ◽  
...  
Keyword(s):  
Real Time ◽  
Flow Condition ◽  
Flow Conditions ◽  
Time Analysis ◽  
Flow Rates ◽  
Microfluidic Platform ◽  
Real Time Analysis ◽  
Controlled Flow ◽  
The One

The contribution focuses on the development of microresonant sensor solution integrated in microfluidic platform for the haemostasis assessment at realistic rheological flow conditions similar to the one in blood vessels. A multi-parameter sensor performs real time analysis of interactions between immobilized collagen and platelets. The detection and characterization of such interactions at controlled flow rates provide information to evaluate the dynamic of each step of primary haemostasis. The microresonant sensor concept was developed and is described in the contribution.

scholarly journals Spectral Intensity Bioaerosol Sensor (SIBS): an instrument for spectrally resolved fluorescence detection of single particles in real time

2019 ◽  
Vol 12 (2) ◽  
pp. 1337-1363 ◽  
Author(s):  
Tobias Könemann ◽  
Nicole Savage ◽  
Thomas Klimach ◽  
David Walter ◽  
Janine Fröhlich-Nowoisky ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Spectra ◽  
Ambient Air ◽  
Spectral Intensity ◽  
Spatiotemporal Variability ◽  
Asymmetry Factor ◽  
Detector Response ◽  
Single Particles ◽  
Spectral Difference ◽  
Wide Range

Abstract. Primary biological aerosol particles (PBAPs) in the atmosphere are highly relevant for the Earth system, climate, and public health. The analysis of PBAPs, however, remains challenging due to their high diversity and large spatiotemporal variability. For real-time PBAP analysis, light-induced fluorescence (LIF) instruments have been developed and widely used in laboratory and ambient studies. The interpretation of fluorescence data from these instruments, however, is often limited by a lack of spectroscopic information. This study introduces an instrument – the Spectral Intensity Bioaerosol Sensor (SIBS; Droplet Measurement Technologies (DMT), Longmont, CO, USA) – that resolves fluorescence spectra for single particles and thus promises to expand the scope of fluorescent PBAP quantification and classification. The SIBS shares key design components with the latest versions of the Wideband Integrated Bioaerosol Sensor (WIBS) and the findings presented here are also relevant for the widely deployed WIBS-4A and WIBS-NEO as well as other LIF instruments. The key features of the SIBS and the findings of this study can be summarized as follows. Particle sizing yields reproducible linear responses for particles in the range of 300 nm to 20 µm. The lower sizing limit is significantly smaller than for earlier commercial LIF instruments (e.g., WIBS-4A and the Ultraviolet Aerodynamic Particle Sizer; UV-APS), expanding the analytical scope into the accumulation-mode size range. Fluorescence spectra are recorded for two excitation wavelengths (λex=285 and 370 nm) and a wide range of emission wavelengths (λmean=302–721 nm) with a resolution of 16 detection channels, which is higher than for most other commercially available LIF bioaerosol sensors. Fluorescence spectra obtained for 16 reference compounds confirm that the SIBS provides sufficient spectral resolution to distinguish major modes of molecular fluorescence. For example, the SIBS resolves the spectral difference between bacteriochlorophyll and chlorophyll a and b. A spectral correction of the instrument-specific detector response is essential to use the full fluorescence emission range. Asymmetry factor (AF) data were assessed and were found to provide only limited analytical information. In test measurements with ambient air, the SIBS worked reliably and yielded characteristically different spectra for single particles in the coarse mode with an overall fluorescent particle fraction of ∼4 % (3σ threshold), which is consistent with earlier studies in comparable environments.

Real-time and decision taking selection of single-particles during automated cryo-EM sessions based on neuro-fuzzy method

2016 ◽  
Vol 55 ◽  
pp. 403-416 ◽  
Author(s):  
David Gil-Carton ◽  
Miguel Zamora ◽  
James D. Sutherland ◽  
Rosa Barrio ◽  
Izaskun Garrido ◽  
...  
Keyword(s):  
Real Time ◽  
Single Particles ◽  
Fuzzy Method ◽  
Neuro Fuzzy ◽  
Decision Taking ◽  
Selection Of

scholarly journals Spectral Intensity Bioaerosol Sensor (SIBS): A new Instrument for Spectrally Resolved Fluorescence Detection of Single Particles in Real-Time

2018 ◽  
Author(s):  
Tobias Könemann ◽  
Nicole Savage ◽  
Thomas Klimach ◽  
David Walter ◽  
Janine Fröhlich-Nowoisky ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Spectra ◽  
Ambient Air ◽  
Spectral Intensity ◽  
Spatiotemporal Variability ◽  
Asymmetry Factor ◽  
Detector Response ◽  
Single Particles ◽  
Wide Range ◽  
New Instrument

Abstract. Primary biological aerosol particles (PBAP) in the atmosphere are highly relevant for the Earth system, climate, and public health. The analysis of PBAP, however, remains challenging due to their high diversity and large spatiotemporal variability. For real-time PBAP analysis, light-induced fluorescence (LIF) instruments have been developed and widely used in laboratory and ambient studies. The interpretation of fluorescence data from these instruments, however, is often limited by a lack of spectroscopic information. This study introduces a new instrument – the Spectral Intensity Bioaerosol Sensor (SIBS) – that resolves fluorescence spectra for single particles and, thus, promises to expand the scope of fluorescent PBAP quantification and classification. The SIBS shares key design components with the latest versions of the Wideband Integrated Bioaerosol Sensor (WIBS) and the findings presented here are also relevant for the widely deployed WIBS-4A and WIBS-NEO as well as other LIF instruments. The key features of the SIBS and findings of this study can be summarized as follows: – Particle sizing yields reproducible linear responses for particles in the range of 300 nm to 20 µm. The lower sizing limit is significantly smaller than for earlier commercial LIF instruments (e.g., WIBS-4A and the Ultraviolet Aerodynamic Particle Sizer (UV-APS)), expanding the analytical scope into the accumulation mode size range. – Fluorescence spectra are recorded for two excitation wavelengths (λex = 285 and 370 nm) and a wide range of emission wavelengths (λmean = 302–721 nm) with a resolution of 16 detection channels, which is higher than for most other commercially available LIF bioaerosol sensors. – Fluorescence spectra obtained for 16 reference compounds confirm that the SIBS provides sufficient spectral resolution to distinguish major modes of molecular fluorescence. For example, the SIBS resolves the spectral difference between bacteriochlorophyll and chlorophyll a/b. – A spectral correction of the instrument-specific detector response is essential to use the full fluorescence emission range. – Asymmetry factor (AF) data were assessed and were found to provide only limited analytical information. – In test measurements with ambient air, the SIBS worked reliably and yielded characteristically different spectra for single particles in the coarse mode with an overall fluorescent particle fraction of ~ 4 % (3σ threshold), which is consistent with earlier studies in comparable environments.

Real-time two-photon lithography in controlled flow to create a single-microparticle array and particle-cluster array for optofluidic imaging

Lab on a Chip ◽  
2018 ◽  
Vol 18 (3) ◽  
pp. 442-450 ◽  
Author(s):  
Bing Xu ◽  
Yang Shi ◽  
Zhaoxin Lao ◽  
Jincheng Ni ◽  
Guoqiang Li ◽  
...  
Keyword(s):  
Real Time ◽  
Biomedical Applications ◽  
Particle Cluster ◽  
Two Photon ◽  
Novel Method ◽  
Controlled Flow

We developed a novel method: real-time two-photon-lithography in controlled flow to achieve 100% one-bead-to-one-trap towards optofluidic imaging and biomedical applications.

In vitro system to study realistic pulsatile flow and stretch signaling in cultured vascular cells

2000 ◽  
Vol 279 (3) ◽  
pp. C797-C805 ◽  
Author(s):  
Xinqi Peng ◽  
Fabio A. Recchia ◽  
Barry J. Byrne ◽  
Ilan S. Wittstein ◽  
Roy C. Ziegelstein ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Real Time ◽  
Aortic Pressure ◽  
Fluorescent Imaging ◽  
Pressure Amplitude ◽  
Vascular Cells ◽  
Glass Capillary ◽  
Controlled Flow

We developed a novel real-time servo-controlled perfusion system that exposes endothelial cells grown in nondistensible or distensible tubes to realistic pulse pressures and phasic shears at physiological mean pressures. A rate-controlled flow pump and linear servo-motor are controlled by digital proportional-integral-derivative feedback that employs previously digitized aortic pressure waves as a command signal. The resulting pressure mirrors the recorded waveform and can be digitally modified to yield any desired mean and pulse pressure amplitude, typically 0–150 mmHg at shears of 0.5–15 dyn/cm2. The system accurately reproduces the desired arterial pressure waveform and cogenerates physiological flow and shears by the interaction of pressure with the tubing impedance. Rectangular glass capillary tubes [1-mm inside diameter (ID)] are used for real-time fluorescent imaging studies (i.e., pHi, NO, Ca2+), whereas silicon distensible tubes (4-mm ID) are used for more chronic (i.e., 2–24 h) studies regarding signal transduction and gene expression. The latter have an elastic modulus of 12.4 · 106 dyn/cm2 similar to in vivo vessels of this size and are studied with the use of a benchtop system. The new approach provides the first in vitro application of realistic mechanical pulsatile forces on vascular cells and should facilitate studies of phasic shear and distension interaction and pulsatile signal transduction.

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