F-mode ultraviolet photoacoustic remote sensing for label-free virtual H&E histopathology using a single excitation wavelength

2021 ◽  
Author(s):  
Pradyumna Kedarisetti ◽  
Brendon Restall ◽  
Nathaniel Haven ◽  
Matthew Martell ◽  
Brendyn Cikaluk ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Excitation Wavelength ◽  
Label Free

Photonic crystals: A platform for label-free and enhanced fluorescence biomolecular and cellular assays

2008 ◽  
Vol 1133 ◽  
Author(s):  
Brian T. Cunningham ◽  
Leo Chan ◽  
Patrick C. Mathias ◽  
Nikhil Ganesh ◽  
Sherine George ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Photonic Crystals ◽  
High Throughput Screening ◽  
High Sensitivity ◽  
Excitation Wavelength ◽  
Label Free ◽  
Crystal Surfaces ◽  
Enhanced Fluorescence ◽  
Preferred Direction ◽  
Label Free Detection

Abstract Photonic crystal surfaces represent a class of resonant optical structures that are capable of supporting high intensity electromagnetic standing waves with near-field and far-field properties that can be exploited for high sensitivity detection of biomolecules and cells. While modulation of the resonant wavelength of a photonic crystal by the dielectric permittivity of adsorbed biomaterials enables label-free detection, the resonance can also be tuned to coincide with the excitation wavelength of common fluorescent tags - including organic molecules and semiconductor quantum dots. Photonic crystals are also capable of efficiently channeling fluorescent emission into a preferred direction for enhanced extraction efficiency. Photonic crystals can be designed to support multiple resonant modes that can perform label free detection, enhanced fluorescence excitation, and enhanced fluorescence extraction simultaneously on the same device. Because photonic crystal surfaces may be inexpensively produced over large surface areas by nanoreplica molding processes, they can be incorporated into disposable labware for applications such as pharmaceutical high throughput screening. In this talk, the optical properties of surface photonic crystals will be reviewed and several applications will be described, including results from screening a 200,000-member chemical compound library for inhibitors of protein-DNA interactions, gene expression microarrays, and high sensitivity of protein biomarkers.

Label-free virtual H&E histopathology using f-mode ultraviolet photoacoustic remote sensing

2021 ◽  
Author(s):  
Pradyumna Kedarisetti ◽  
Nathaniel J. M. Haven ◽  
Brendon S. Restall ◽  
Matthew T. Martell ◽  
Roger J. Zemp
Keyword(s):  
Remote Sensing ◽  
Label Free

scholarly journals Application of Aluminum Hydroxide for Improvement of Label-Free SERS Detection of Some Cephalosporin Antibiotics in Urine

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 91 ◽  
Author(s):  
Natalia E. Markina ◽  
Alexey V. Markin
Keyword(s):  
Aluminum Hydroxide ◽  
Surface Enhanced Raman Spectroscopy ◽  
Negative Influence ◽  
Quantitative Detection ◽  
Therapeutic Drug ◽  
Excitation Wavelength ◽  
Sers Substrate ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Sers Detection

This report is dedicated to development of surface-enhanced Raman spectroscopy (SERS) based analysis protocol for detection of antibiotics in urine. The key step of the protocol is the pretreatment of urine before the detection to minimize background signal. The pretreatment includes extraction of intrinsic urine components using aluminum hydroxide gel (AHG) and further pH adjusting of the purified sample. The protocol was tested by detection of a single antibiotic in artificially spiked samples of real urine. Five antibiotics of cephalosporin class (cefazolin, cefoperazone, cefotaxime, ceftriaxone, and cefuroxime) were used for testing. SERS measurements were performed using a portable Raman spectrometer with 638 nm excitation wavelength and silver nanoparticles as SERS substrate. The calibration curves of four antibiotics (cefuroxime is the exception) cover the concentrations required for detection in patient’s urine during therapy (25/100‒500 μg/mL). Random error of the analysis (RSD < 20%) and limits of quantification (20‒90 μg/mL) for these antibiotics demonstrate the applicability of the protocol for reliable quantitative detection during therapeutic drug monitoring. The detection of cefuroxime using the protocol is not sensitive enough, allowing only for qualitative detection. Additionally, time stability and batch-to-batch reproducibility of AHG were studied and negative influence of the pretreatment protocol and its limitations were estimated and discussed.

Label-free non-contact imaging of cell nuclei using ultraviolet photoacoustic remote sensing microscopy (Conference Presentation)

2019 ◽  
Author(s):  
Nathaniel J. M. Haven ◽  
Kevan L. Bell ◽  
Pradyumna Kedarisetti ◽  
Parsin Haji Reza ◽  
John D. Lewis ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Label Free ◽  
Cell Nuclei ◽  
Contact Imaging

scholarly journals THE EFFECTIVE OF DIFFERENT EXCITATION WAVELENGTHS ON THE IDENTIFICATION OF PLANT SPECIES BASED ON FLUORESCENCE LIDAR

2016 ◽  
Vol XLI-B1 ◽  
pp. 147-150
Author(s):  
Jian Yang ◽  
Wei Gong ◽  
Shuo Shi ◽  
Lin Du ◽  
Jia Sun ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Plant Species ◽  
Fluorescence Spectra ◽  
Principal Component ◽  
Laser Induced Fluorescence ◽  
Support Vector ◽  
Excitation Wavelength ◽  
Lidar System ◽  
Excitation Light

Laser-induced fluorescence (LIF) served as an active technology has been widely used in many field, and it is closely related to excitation wavelength (EW). The objective of this investigation is to discuss the performance of different EWs of LIF LiDAR in identifying plant species. In this study, the 355, 460 and 556 nm lasers were utilized to excite the leaf fluorescence and the fluorescence spectra were measured by using the LIF LiDAR system built in the laboratory. Subsequently, the principal component analysis (PCA) with the help of support vector machine (SVM) was utilized to analyse fluorescence spectra. For the three EWs, the overall identification rates of the six plant species were 80 %, 83.3 % and 90 %. Experimental results demonstrated that 556 nm excitation light source is superior to 355 and 460 nm for the classification of the plant species for the same genus in this study. Thus, an appropriate excitation wavelength should be considered when the LIF LiDAR was utilized in the field of remote sensing based on the LIF technology.

scholarly journals Circular Intensity Differential Scattering for Label-Free Chromatin Characterization: A Review for Optical Microscopy

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2428
Author(s):  
Aymeric Le Gratiet ◽  
Riccardo Marongiu ◽  
Alberto Diaspro
Keyword(s):  
Optical Microscopy ◽  
Gold Standard ◽  
Molecular Conformation ◽  
Polarized Light ◽  
Excitation Wavelength ◽  
General Description ◽  
Label Free ◽  
Differential Measurement ◽  
Scale Down

Circular Intensity Differential Scattering (CIDS) provides a differential measurement of the circular right and left polarized light and has been proven to be a gold standard label-free technique to study the molecular conformation of complex biopolymers, such as chromatin. In early works, it has been shown that the scattering component of the CIDS signal gives information from the long-range chiral organization on a scale down to 1/10th–1/20th of the excitation wavelength, leading to information related to the structure and orientation of biopolymers in situ at the nanoscale. In this paper, we review the typical methods and technologies employed for measuring this signal coming from complex macro-molecules ordering. Additionally, we include a general description of the experimental architectures employed for spectroscopic CIDS measurements, angular or spectral, and of the most recent advances in the field of optical imaging microscopy, allowing a visualization of the chromatin organization in situ.

scholarly journals Grating-assisted coupling enhancing plasmonic tip nanofocusing illuminated via radial vector beam

Nanophotonics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 2303-2311 ◽  
Author(s):  
Fanfan Lu ◽  
Wending Zhang ◽  
Jiachen Zhang ◽  
Min Liu ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Electric Field ◽  
Near Field ◽  
Numerical Aperture ◽  
Field Enhancement ◽  
Super Resolution ◽  
Decisive Role ◽  
Excitation Wavelength ◽  
Label Free ◽  
Vector Beam ◽  
Simulation Results

AbstractTip-enhanced Raman spectroscopy (TERS) is a very useful method to achieve label-free and super-resolution imaging, and the plasmonic tip nanofocusing plays a decisive role for TERS performance. Here, we present a method to enhance the nanofocusing characteristic of a plasmonic tip integrated in a grating near the tip apex. Simulation results show that the grating near the tip apex can significantly improve the electric field intensity of the nanofocusing field compared with a conventional bare tip, under axial excitation of a tightly focused radial vector beam. The electric field enhancement characteristic is quantified in relation with the groove number of grating, excitation wavelength, period of grating, and numerical aperture of the micro-objective (MO). These simulation results could be a good reference to fabricate a plasmonic tip for TERS applications, which is an effective way to promote the development of tip-enhanced near-field optical microscopy.

scholarly journals Phenotypic Profiling of Antibiotic Response Signatures in Escherichia coli Using Raman Spectroscopy

2013 ◽  
Vol 58 (3) ◽  
pp. 1302-1314 ◽  
Author(s):  
A. I. M. Athamneh ◽  
R. A. Alajlouni ◽  
R. S. Wallace ◽  
M. N. Seleem ◽  
R. S. Senger
Keyword(s):  
Escherichia Coli ◽  
Raman Spectroscopy ◽  
Mechanism Of Action ◽  
Excitation Wavelength ◽  
Label Free ◽  
Analysis Model ◽  
Content Type ◽  
E Coli ◽  
Antibiotic Drug ◽  
Drug Development Research

ABSTRACTIdentifying the mechanism of action of new potential antibiotics is a necessary but time-consuming and costly process. Phenotypic profiling has been utilized effectively to facilitate the discovery of the mechanism of action and molecular targets of uncharacterized drugs. In this research, Raman spectroscopy was used to profile the phenotypic response ofEscherichia colito applied antibiotics. The use of Raman spectroscopy is advantageous because it is noninvasive, label free, and prone to automation, and its results can be obtained in real time. In this research,E. colicultures were subjected to three times the MICs of 15 different antibiotics (representing five functional antibiotic classes) with known mechanisms of action for 30 min before being analyzed by Raman spectroscopy (using a 532-nm excitation wavelength). The resulting Raman spectra contained sufficient biochemical information to distinguish between profiles induced by individual antibiotics belonging to the same class. The collected spectral data were used to build a discriminant analysis model that identified the effects of unknown antibiotic compounds on the phenotype ofE. colicultures. Chemometric analysis showed the ability of Raman spectroscopy to predict the functional class of an unknown antibiotic and to identify individual antibiotics that elicit similar phenotypic responses. Results of this research demonstrate the power of Raman spectroscopy as a cellular phenotypic profiling methodology and its potential impact on antibiotic drug development research.

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