scholarly journals Shaping field for 3D laser scanning microscopy

2015 ◽  
Vol 40 (14) ◽  
pp. 3300 ◽  
Author(s):  
Jorge Colon ◽  
Hyungsik Lim
2015 ◽  
Vol 96 ◽  
pp. 304-313 ◽  
Author(s):  
Stefanie Funke ◽  
Julia Matilainen ◽  
Heiko Nalenz ◽  
Karoline Bechtold-Peters ◽  
Hanns-Christian Mahler ◽  
...  

2014 ◽  
Vol 33 (5) ◽  
pp. 353-361
Author(s):  
Klaus Weber ◽  
Alexander Waletzky ◽  
Diana Fendl ◽  
Patricia Ordóñez ◽  
Pradeep Takawale ◽  
...  

Sperm analysis is one of the end points in reproductive toxicology studies. Different methods for quantitative sperm analysis have been described. For qualitative morphological sperm analysis, either such techniques or smears of sperm and histological sperm staging are in use. Any of these methods provides morphological results on a light microscopy level. Laser scanning microscopy is a technique using a focused laser for scanning an object. The Olympus 3D Laser Scanning Microscope LEXT OLS4000 with optional possibilities of differential interference contrast provides a microscopic method for visualizing microasperities, which are far beyond the resolving power of a typical light or laser microscope. This technique was applied to sperm of mice, rats, rabbits, and cynomolgus monkeys at magnifications up to ×17 090. The obtained images are comparable to those of a scanning electron microscope under relatively low-power magnifications. Measurements on sperm parameters were taken by an integrated image analysis software tool. Abnormalities were easily detectable.


2014 ◽  
Vol 229 ◽  
pp. S228
Author(s):  
Klaus Weber ◽  
Diana Fendl ◽  
Felix Hein ◽  
Andres König ◽  
Marc Kunze ◽  
...  

2012 ◽  
Vol 11 (3) ◽  
pp. 669-674 ◽  
Author(s):  
Szabolcs Szilveszter ◽  
Botond Raduly ◽  
Szilard Bucs ◽  
Beata Abraham ◽  
Szabolcs Lanyi ◽  
...  

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