scholarly journals Optical topography of rough surfaces using vortex localization of fluorescent markers

2020 ◽  
Vol 45 (16) ◽  
pp. 4468
Author(s):  
Petr Schovánek ◽  
Petr Bouchal ◽  
Zdeněk Bouchal
Keyword(s):  
Rough Surfaces ◽  
Fluorescent Markers ◽  
Optical Topography

scholarly journals Comparison of three focus sensors for optical topography measurement of rough surfaces

2019 ◽  
Vol 27 (23) ◽  
pp. 33459 ◽  
Author(s):  
Martin Šarbort ◽  
Miroslava Holá ◽  
Jan Pavelka ◽  
Petr Schovánek ◽  
Šimon Řeřucha ◽  
...  
Keyword(s):  
Rough Surfaces ◽  
Optical Topography

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

1989 ◽  
Vol 47 ◽  
pp. 146-147
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Pyramidal Neurons ◽  
Light And Electron Microscopy ◽  
Scanning Laser ◽  
Laser Exposure ◽  
Confocal Scanning Laser Microscope ◽  
Fluorescent Markers ◽  
Before And After ◽  
Staining Protocol ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

Infrared spectra of carbon monoxide on platinum, iridium and copper ; comparison of flat and rough surfaces

1981 ◽  
Vol 78 ◽  
pp. 927-932 ◽  
Author(s):  
F.J.C.M. Toolenaar ◽  
G.J. van der Poort ◽  
F. Stoop ◽  
V. Ponec
Keyword(s):  
Carbon Monoxide ◽  
Infrared Spectra ◽  
Rough Surfaces

OPTICAL PROPERTIES OF THIN FILMS ON ROUGH SURFACES

1983 ◽  
Vol 44 (C10) ◽  
pp. C10-363-C10-366 ◽  
Author(s):  
J. Vlieger ◽  
M. M. Wind
Keyword(s):  
Thin Films ◽  
Optical Properties ◽  
Rough Surfaces

A Laboratory Device to Measure the Interfacial Phenomena between Rubber and Rough Surfaces

1999 ◽  
Vol 27 (4) ◽  
pp. 206-226 ◽  
Author(s):  
L. Garro ◽  
G. Gurnari ◽  
G. Nicoletto ◽  
A. Serra
Keyword(s):  
Rough Surfaces ◽  
Computer Software ◽  
Interfacial Phenomena ◽  
New Device ◽  
The Road ◽  
Constant Torque ◽  
Rubber Compounds ◽  
Simple Geometry ◽  
Tangential Forces ◽  
On The Road

Abstract The interfacial phenomena between tread rubber compounds and rough surfaces are responsible for most of the behavior of a tire on the road. A new device was developed for the investigation of these phenomena in the laboratory. The device consists of a fully instrumented road wheel on which a simple geometry specimen is driven. The possibilities offered by this device are to perform tests at constant slip or at constant torque on both wet and dry surfaces, with complex cycles. The machine allows the measurement of slip, tangential forces, and temperature on the specimen, and computer software adds the possibility of applying Fourier analyses on force, road wheel speed, and specimen speed data. Other possibilities offered by the road wheel are to change the road surface, the load on the specimen, and the water rate. The description of a complete experiment is detailed in the paper showing the correlation of data with actual tire performances.

The Markov Model for Alteration of Rough Surfaces during Their Mechanical Interaction

Vestnik MEI ◽  
2017 ◽  
pp. 101-110
Author(s):  
Yuri A. Goritskiy ◽  
◽  
Konstantin V. Gavrilov ◽  
Yulia S. Ismailova ◽  
Olga V. Shevchenko ◽  
...  
Keyword(s):  
Markov Model ◽  
Rough Surfaces ◽  
Mechanical Interaction

A Fused Thiophene-S,S-Dioxide-Based Super-Photostable Fluorescent Marker for Lipid Droplets

2020 ◽  
Author(s):  
Masayasu Taki ◽  
Keiji Kajiwara ◽  
Eriko Yamaguchi ◽  
Yoshikatsu Sato ◽  
Shigehiro Yamaguchi
Keyword(s):  
Small Molecules ◽  
Lipid Droplets ◽  
Trajectory Analysis ◽  
Super Resolution ◽  
Fluorescent Marker ◽  
Selective Staining ◽  
Fluorescent Markers ◽  
Biological Studies ◽  
Particle Level

Lipid droplets (LDs) are essential organelle in most eukaryotes, and tracking intracellular LDs dynamics using synthetic small molecules is crucial for biological studies. However, only a limited number of fluorescent markers that satisfy all requirements, such as the selective staining of LDs, high photostability, and sufficient biocompatibility, have been developed. Herein, we report a series of donor-p-acceptor dyes based on the thiophene-containing fused polycyclic scaffold [1]benzothieno[3,2-<i>b</i>][1]benzothiophene (BTBT), in which either or both thiophene rings are oxidized into thiophene-<i>S</i>,<i>S</i>-dioxide to form an electron-accepting building block. Among these dyes, LAQ1 satisfied all the aforementioned requirements, and allowed us capturing ultra-small LDs on the endoplasmic reticulum (ER) membrane by stimulation emission depletion (STED) microscopy with a super-resolution below the diffraction limit of light. Moreover, the extremely high photostability of LAQ1 enabled recording the lipolysis of LDs and the concomitant lipogenesis as well as long-term trajectory analysis of micro LDs at the single particle level in living cells.

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