Image reconstruction for large FOV Airy beam light-sheet microscopy by a 3D deconvolution approach

2020 ◽  
Vol 45 (10) ◽  
pp. 2804
Author(s):  
Shun Qin
Keyword(s):  
Image Reconstruction ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Airy Beam

scholarly journals Achromatic terahertz Airy beam generation with dielectric metasurfaces

Nanophotonics ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Qingqing Cheng ◽  
Juncheng Wang ◽  
Ling Ma ◽  
Zhixiong Shen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Biomedical Imaging ◽  
Frequency Dispersion ◽  
Light Sheet ◽  
Photonic Devices ◽  
Self Healing ◽  
Beam Focusing ◽  
Light Sheet Microscopy ◽  
Airy Beam ◽  
Potential Applications ◽  
Airy Beams

AbstractAiry beams exhibit intriguing properties such as nonspreading, self-bending, and self-healing and have attracted considerable recent interest because of their many potential applications in photonics, such as to beam focusing, light-sheet microscopy, and biomedical imaging. However, previous approaches to generate Airy beams using photonic structures have suffered from severe chromatic problems arising from strong frequency dispersion of the scatterers. Here, we design and fabricate a metasurface composed of silicon posts for the frequency range 0.4–0.8 THz in transmission mode, and we experimentally demonstrate achromatic Airy beams exhibiting autofocusing properties. We further show numerically that a generated achromatic Airy-beam-based metalens exhibits self-healing properties that are immune to scattering by particles and that it also possesses a larger depth of focus than a traditional metalens. Our results pave the way to the realization of flat photonic devices for applications to noninvasive biomedical imaging and light-sheet microscopy, and we provide a numerical demonstration of a device protocol.

scholarly journals Light-Sheet Microscopy using Attenuation-Compensating Airy Beam

2017 ◽  
Vol 112 (3) ◽  
pp. 145a
Author(s):  
Cong Liu ◽  
Yen-Liang Liu ◽  
Tim Yeh
Keyword(s):  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Airy Beam

scholarly journals Light-sheet microscopy using an Airy beam

2014 ◽  
Vol 11 (5) ◽  
pp. 541-544 ◽  
Author(s):  
Tom Vettenburg ◽  
Heather I C Dalgarno ◽  
Jonathan Nylk ◽  
Clara Coll-Lladó ◽  
David E K Ferrier ◽  
...  
Keyword(s):  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Airy Beam

scholarly journals Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

OSA Continuum ◽  
2020 ◽  
Vol 3 (4) ◽  
pp. 1068
Author(s):  
Stella Corsetti ◽  
Philip Wijesinghe ◽  
Persephone B. Poulton ◽  
Shuzo Sakata ◽  
Khushi Vyas ◽  
...  
Keyword(s):  
Deep Learning ◽  
Super Resolution ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Airy Beam

Enhancement of image quality in planar Airy light-sheet microscopy via subtraction method

2021 ◽  
Author(s):  
Suhui Deng ◽  
Liusong Yuan ◽  
Peiwei Cheng ◽  
Yuhao Wang ◽  
Mingping Liu
Keyword(s):  
Image Quality ◽  
Light Sheet ◽  
Large Field ◽  
Subtraction Method ◽  
Optical Sectioning ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Airy Beam ◽  
Photon Excitation ◽  
Distribution Uniformity

Abstract The use of propagation-invariant Airy beams enables a light-sheet microscopy with a large field-of-view. Without relying upon two-photon excitation or deconvolution-based processing to eliminate out-of focus blur caused by the side lobes, here, we present how the subtraction method is applied to enhance the image quality in digital scanned light-sheet microscopy with Airy beam. In the proposed method, planar Airy beam with the symmetric transversal structure is used to excite the sample. A hollow Airy beam with zero intensity at the focal plane is created, which is mainly used to excite the out-of-focus signal. By scanning the sample twice with the normal planar Airy beam and the hollow Airy beam, digital post-processing of the obtained images by subtraction allows for the rejection of out-of-focus blur and improves the optical sectioning, the axial resolution and the intensity distribution uniformity of the light-sheet microscopy.

Airy Beam Light Sheet Microscopy with Deconvolution

2017 ◽  
Vol 37 (3) ◽  
pp. 0318013
Author(s):  
徐豪 Xu Hao ◽  
张运海 Zhang Yunhai ◽  
张欣 Zhang Xin ◽  
肖昀 Xiao Yun ◽  
黄维 Huang Wei
Keyword(s):  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Airy Beam

scholarly journals Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

2020 ◽  
Author(s):  
Stella Corsetti ◽  
Philip Wijesinghe ◽  
Persephone B. Poulton ◽  
Shuzo Sakata ◽  
Khushi Vyas ◽  
...  
Keyword(s):  
Deep Learning ◽  
High Resolution ◽  
Dynamic Range ◽  
Tissue Sample ◽  
Super Resolution ◽  
Light Sheet ◽  
Wide Field ◽  
Light Sheet Microscopy ◽  
Airy Beam ◽  
Model Of Alzheimer’S Disease

AbstractImaging across length scales and in depth has been an important pursuit of widefield optical imaging. This promises to reveal fine cellular detail within a widefield snapshot of a tissue sample. Current advances often sacrifice resolution through selective sub-sampling to provide a wide field of view in a reasonable time scale. We demonstrate a new avenue for recovering high-resolution images from sub-sampled data in light-sheet microscopy using deep-learning super-resolution. We combine this with the use of a widefield Airy beam to achieve high-resolution imaging over extended fields of view and depths. We characterise our method on fluorescent beads as test targets. We then demonstrate improvements in imaging amyloid plaques in a cleared brain from a mouse model of Alzheimer’s disease, and in excised healthy and cancerous colon and breast tissues. This development can be widely applied in all forms of light sheet microscopy to provide a two-fold increase in the dynamic range of the imaged length scale. It has the potential to provide further insight into neuroscience, developmental biology and histopathology.

scholarly journals Single-Molecule Light-Sheet Microscopy with Local Nanopipette Delivery

2021 ◽  
Vol 93 (8) ◽  
pp. 4092-4099
Author(s):  
Bing Li ◽  
Aleks Ponjavic ◽  
Wei-Hsin Chen ◽  
Lee Hopkins ◽  
Craig Hughes ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Sheet ◽  
Light Sheet Microscopy
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