3D high resolution generative deep-learning network for fluorescence microscopy imaging

2020 ◽  
Vol 45 (7) ◽  
pp. 1695 ◽  
Author(s):  
Hang Zhou ◽  
Ruiyao Cai ◽  
Tingwei Quan ◽  
Shijie Liu ◽  
Shiwei Li ◽  
...  
2020 ◽  
Vol 12 (15) ◽  
pp. 2345 ◽  
Author(s):  
Ahram Song ◽  
Yongil Kim ◽  
Youkyung Han

Object-based image analysis (OBIA) is better than pixel-based image analysis for change detection (CD) in very high-resolution (VHR) remote sensing images. Although the effectiveness of deep learning approaches has recently been proved, few studies have investigated OBIA and deep learning for CD. Previously proposed methods use the object information obtained from the preprocessing and postprocessing phase of deep learning. In general, they use the dominant or most frequently used label information with respect to all the pixels inside an object without considering any quantitative criteria to integrate the deep learning network and object information. In this study, we developed an object-based CD method for VHR satellite images using a deep learning network to denote the uncertainty associated with an object and effectively detect the changes in an area without the ground truth data. The proposed method defines the uncertainty associated with an object and mainly includes two phases. Initially, CD objects were generated by unsupervised CD methods, and the objects were used to train the CD network comprising three-dimensional convolutional layers and convolutional long short-term memory layers. The CD objects were updated according to the uncertainty level after the learning process was completed. Further, the updated CD objects were considered as the training data for the CD network. This process was repeated until the entire area was classified into two classes, i.e., change and no-change, with respect to the object units or defined epoch. The experiments conducted using two different VHR satellite images confirmed that the proposed method achieved the best performance when compared with the performances obtained using the traditional CD approaches. The method was less affected by salt and pepper noise and could effectively extract the region of change in object units without ground truth data. Furthermore, the proposed method can offer advantages associated with unsupervised CD methods and a CD network subjected to postprocessing by effectively utilizing the deep learning technique and object information.


2019 ◽  
Author(s):  
Zhou Hang ◽  
Li Shiwei ◽  
Huang Qing ◽  
Liu Shijie ◽  
Quan Tingwei ◽  
...  

AbstractDeep learning technology enables us acquire high resolution image from low resolution image in biological imaging free from sophisticated optical hardware. However, current methods require a huge number of the precisely registered low-resolution (LR) and high-resolution (HR) volume image pairs. This requirement is challengeable for biological volume imaging. Here, we proposed 3D deep learning network based on dual generative adversarial network (dual-GAN) framework for recovering HR volume images from LR volume images. Our network avoids learning the direct mappings from the LR and HR volume image pairs, which need precisely image registration process. And the cycle consistent network makes the predicted HR volume image faithful to its corresponding LR volume image. The proposed method achieves the recovery of 20x/1.0 NA volume images from 5x/0.16 NA volume images collected by light-sheet microscopy. In essence our method is suitable for the other imaging modalities.


2021 ◽  
Vol 11 (1) ◽  
pp. 339-348
Author(s):  
Piotr Bojarczak ◽  
Piotr Lesiak

Abstract The article uses images from Unmanned Aerial Vehicles (UAVs) for rail diagnostics. The main advantage of such a solution compared to traditional surveys performed with measuring vehicles is the elimination of decreased train traffic. The authors, in the study, limited themselves to the diagnosis of hazardous split defects in rails. An algorithm has been proposed to detect them with an efficiency rate of about 81% for defects not less than 6.9% of the rail head width. It uses the FCN-8 deep-learning network, implemented in the Tensorflow environment, to extract the rail head by image segmentation. Using this type of network for segmentation increases the resistance of the algorithm to changes in the recorded rail image brightness. This is of fundamental importance in the case of variable conditions for image recording by UAVs. The detection of these defects in the rail head is performed using an algorithm in the Python language and the OpenCV library. To locate the defect, it uses the contour of a separate rail head together with a rectangle circumscribed around it. The use of UAVs together with artificial intelligence to detect split defects is an important element of novelty presented in this work.


2021 ◽  
Vol 11 (13) ◽  
pp. 5880
Author(s):  
Paloma Tirado-Martin ◽  
Raul Sanchez-Reillo

Nowadays, Deep Learning tools have been widely applied in biometrics. Electrocardiogram (ECG) biometrics is not the exception. However, the algorithm performances rely heavily on a representative dataset for training. ECGs suffer constant temporal variations, and it is even more relevant to collect databases that can represent these conditions. Nonetheless, the restriction in database publications obstructs further research on this topic. This work was developed with the help of a database that represents potential scenarios in biometric recognition as data was acquired in different days, physical activities and positions. The classification was implemented with a Deep Learning network, BioECG, avoiding complex and time-consuming signal transformations. An exhaustive tuning was completed including variations in enrollment length, improving ECG verification for more complex and realistic biometric conditions. Finally, this work studied one-day and two-days enrollments and their effects. Two-days enrollments resulted in huge general improvements even when verification was accomplished with more unstable signals. EER was improved in 63% when including a change of position, up to almost 99% when visits were in a different day and up to 91% if the user experienced a heartbeat increase after exercise.


Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1156
Author(s):  
Kang Hee Lee ◽  
Sang Tae Choi ◽  
Guen Young Lee ◽  
You Jung Ha ◽  
Sang-Il Choi

Axial spondyloarthritis (axSpA) is a chronic inflammatory disease of the sacroiliac joints. In this study, we develop a method for detecting bone marrow edema by magnetic resonance (MR) imaging of the sacroiliac joints and a deep-learning network. A total of 815 MR images of the sacroiliac joints were obtained from 60 patients diagnosed with axSpA and 19 healthy subjects. Gadolinium-enhanced fat-suppressed T1-weighted oblique coronal images were used for deep learning. Active sacroiliitis was defined as bone marrow edema, and the following processes were performed: setting the region of interest (ROI) and normalizing it to a size suitable for input to a deep-learning network, determining bone marrow edema using a convolutional-neural-network-based deep-learning network for individual MR images, and determining sacroiliac arthritis in subject examinations based on the classification results of individual MR images. About 70% of the patients and normal subjects were randomly selected for the training dataset, and the remaining 30% formed the test dataset. This process was repeated five times to calculate the average classification rate of the five-fold sets. The gradient-weighted class activation mapping method was used to validate the classification results. In the performance analysis of the ResNet18-based classification network for individual MR images, use of the ROI showed excellent detection performance of bone marrow edema with 93.55 ± 2.19% accuracy, 92.87 ± 1.27% recall, and 94.69 ± 3.03% precision. The overall performance was additionally improved using a median filter to reflect the context information. Finally, active sacroiliitis was diagnosed in individual subjects with 96.06 ± 2.83% accuracy, 100% recall, and 94.84 ± 3.73% precision. This is a pilot study to diagnose bone marrow edema by deep learning based on MR images, and the results suggest that MR analysis using deep learning can be a useful complementary means for clinicians to diagnose bone marrow edema.


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