Ultrafast ultrasound imaging of surface acoustic waves induced by laser excitation compared with acoustic radiation force

2020 ◽  
Vol 45 (7) ◽  
pp. 1810
Author(s):  
Lingyi Zhao ◽  
Don Vanderlaan ◽  
Heechul Yoon ◽  
Jingfei Liu ◽  
Changhui Li ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Acoustic Waves ◽  
Laser Excitation ◽  
Acoustic Radiation ◽  
Surface Acoustic Waves ◽  
Radiation Force ◽  
Acoustic Radiation Force

O-124 Contact-free oocyte denudation in a chip-scale ultrasonic microfluidic device

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Mokhtare ◽  
P Xie ◽  
B Davaji ◽  
A Abbaspourrad ◽  
Z Rosenwaks ◽  
...  
Keyword(s):  
Mechanical Stress ◽  
Embryo Development ◽  
Microfluidic Device ◽  
Acoustic Waves ◽  
Acoustic Radiation ◽  
Gas Permeability ◽  
Surface Acoustic Waves ◽  
Radiation Force ◽  
Ultrasonic Waves ◽  
Acoustic Radiation Force

Abstract Study question To design and test an automated microfluidic device to revolutionize the cumulus-oocyte-complex (COC) denudation procedure for intracytoplasmic sperm injection (ICSI) using murine oocytes. Summary answer Oocyte exposure to temperature variation, mechanical stress, and prolonged chemical treatment during denudation was mitigated using our microfluidic device based on surface acoustic waves (SAWs). What is known already COC denudation is a prerequisite for many ART procedures such as ICSI. However, this procedure is based on manual pipetting (MP), which lacks standardization and requires experienced embryologists to perform. Inadequate MP may damage oocytes through prolonged enzymatic treatment or high fluidic stresses and may jeopardize gamete competence. The use of microfluidic devices based on porous membranes or microchannels has been adopted by many laboratories for sperm selection. Of these, microchannel devices may also be adapted for denudation with minimal mechanical stress in a controlled microenvironment. However, oocyte manipulation and extraction have proven difficult to achieve. Study design, size, duration We developed a novel ultrasonic microfluidic device based on a microwell design manufactured with Polydimethylsiloxane (PDMS). The SAWs were generated by 4 interdigitated transducers (IDTs) arranged in an orthogonally symmetric pattern. A non-toxic dosage of ultrasonic waves, similar to those used in gynecology and obstetrics, was applied. COCs were denuded by induced acoustic streaming and acoustic radiation force. Denudation rate, embryo development, and pregnancy outcomes were assessed and compared to control oocytes denuded by MP. Participants/materials, setting, methods For each run, up to 10 individual COCs from super-ovulated B6D2F1 mice were loaded into the microwell alongside diluted hyaluronidase (20 IU/ml) and denuded by 80 or 200 MHz SAWs. Denuded oocytes were fertilized by piezo-actuated ICSI using spermatozoa from the same strain. Pre-implantation embryo development was assessed in a time-lapse incubator for up to 96 h. High-quality blastocysts were transferred to 2.5-dpc pseudo-pregnant CD-1 surrogates. Pregnancy and offspring health were observed. Main results and the role of chance Using alternating frequency sweep in a pulse-repetition mode, we swirled the fluid inside the microwell consistently and tumbled COCs inside the microwell to expose them to acoustic steaming-induced drag forces and acoustic radiation force. Using a high-speed camera and particle-tracking technique, we observed that the drag force generated by the SAWs fulfilled the denudation mechanism. Additionally, due to the small attenuation coefficient in water, thermal absorption heating remains minuscule, preventing any thermal-induced damage. Our device significantly reduced the time and labor of the denudation process. It also yielded proper denudation quality without oocyte loss. To ensure that SAWs do not damage oocytes, 40 oocytes denuded by 80 MHz SAWs, 25 oocytes denuded by 200 MHz SAWs, and 30 oocytes denuded by MP were inseminated by piezo-actuated ICSI. The 80-MHz, 200-MHz, and MP groups yielded comparable post-ICSI survival (82.5% vs. 84.0% vs. 83.3%, respectively), fertilization (80.0% vs. 80.0% vs. 83.3%,respectively), and blastulation rates (72.5% vs. 82.0% vs. 66.7%, respectively). Embryo morphokinetics were also not impacted. After transferring all blastocysts into recipient mice, 8 live births were achieved from the 80-MHz group, while 5 were achieved from the 200-MHz group. Limitations, reasons for caution Although PDMS is a popular material due to its high optical transparency and biocompatibility, adverse effects due to gas permeability and small-molecule adsorption cannot be excluded. Large-scale mouse embryo assays should be performed to assess the teratogenicity of PDMS. Operation parameters must be optimized for human COCs in clinical application. Wider implications of the findings Adopting widely used ultrasound techniques with emerging SAW technology is a major step toward advancing and standardizing oocyte denudation—a laborious yet delicate procedure. We predict it will be further integrated with AI and miniaturized robotics, modules specialized in gamete assessment, ICSI, and embryo evaluation in the near future. Trial registration number ‘not applicable’

scholarly journals Acoustophoretic Control of Microparticle Transport Using Dual-Wavelength Surface Acoustic Wave Devices

Micromachines ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 52 ◽  
Author(s):  
Jin-Chen Hsu ◽  
Chih-Hsun Hsu ◽  
Yeo-Wei Huang
Keyword(s):  
Particle Motion ◽  
Acoustic Waves ◽  
Acoustic Pressure ◽  
Acoustic Radiation ◽  
Surface Acoustic Waves ◽  
Microfluidic Channel ◽  
Radiation Force ◽  
Dual Wavelength ◽  
Single Frequency ◽  
Motion Trajectories

We present a numerical and experimental study of acoustophoretic manipulation in a microfluidic channel using dual-wavelength standing surface acoustic waves (SSAWs) to transport microparticles into different outlets. The SSAW fields were excited by interdigital transducers (IDTs) composed of two different pitches connected in parallel and series on a lithium niobate substrate such that it yielded spatially superimposed and separated dual-wavelength SSAWs, respectively. SSAWs of a singltablee target wavelength can be efficiently excited by giving an RF voltage of frequency determined by the ratio of the velocity of the SAW to the target IDT pitch (i.e., f = cSAW/p). However, the two-pitch IDTs with similar pitches excite, less efficiently, non-target SSAWs with the wavelength associated with the non-target pitch in addition to target SSAWs by giving the target single-frequency RF voltage. As a result, dual-wavelength SSAWs can be formed. Simulated results revealed variations of acoustic pressure fields induced by the dual-wavelength SSAWs and corresponding influences on the particle motion. The acoustic radiation force in the acoustic pressure field was calculated to pinpoint zero-force positions and simulate particle motion trajectories. Then, dual-wavelength SSAW acoustofluidic devices were fabricated in accordance with the simulation results to experimentally demonstrate switching of SSAW fields as a means of transporting particles. The effects of non-target SSAWs on pre-actuating particles were predicted and observed. The study provides the design considerations needed for the fabrication of acoustofluidic devices with IDT-excited multi-wavelength SSAWs for acoustophoresis of microparticles.

Acoustic radiation force impulse (ARFI) ultrasound imaging of pancreatic cystic lesions

2011 ◽  
Vol 80 (2) ◽  
pp. 241-244 ◽  
Author(s):  
M. D’Onofrio ◽  
A. Gallotti ◽  
R. Salvia ◽  
P. Capelli ◽  
R. Pozzi Mucelli
Keyword(s):  
Ultrasound Imaging ◽  
Acoustic Radiation ◽  
Radiation Force ◽  
Acoustic Radiation Force ◽  
Acoustic Radiation Force Impulse ◽  
Cystic Lesions

scholarly journals Measurement of the acoustic streaming pattern in a standing surface acoustic wave field

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Sebastian Sachs ◽  
Christian Cierpka ◽  
Jörg König
Keyword(s):  
Wave Field ◽  
Acoustic Waves ◽  
Acoustic Radiation ◽  
Electrical Power ◽  
Surface Acoustic Waves ◽  
Acoustic Streaming ◽  
Fluid Motion ◽  
Three Dimensional ◽  
Radiation Force ◽  
Flow Measurements

The application of standing surface acoustic waves (sSAW) has enabled the development of many flexible and easily scalable concepts for the fractionation of particle solutions in the field of microfluidic lab-ona-chip devices. In this context, the acoustic radiation force (ARF) is often employed for the targeted manipulation of particle trajectories, whereas acoustically induced flows complicate efficient fractionation in many systems [Sehgal and Kirby (2017)]. Therefore, a characterization of the superimposed fluid motion is essential for the design of such devices. The present work focuses on a structural analysis of the acousticallyexcited flow, both in the center and in the outer regions of the standing wave field. For this, experimental flow measurements were conducted using astigmatism particle tracking velocimetry (APTV) [Cierpka et al. (2010)]. Through multiple approaches, we address the specific challenges for reliable velocity measurements in sSAW due to limited optical access, the influence of the ARF on particle motion, and regions of particle depletion caused by multiple pressure nodes along the channel width and height. Variations in frequency, channel geometry, and electrical power allow for conclusions to be drawn on the formation of a complex, three-dimensional vortex structure at the beginning and end of the sSAW.

scholarly journals Theoretical Framework of Radiation Force in Surface Acoustic Waves for Modulated Particle Sorting

2019 ◽  
Vol 63 (2) ◽  
pp. 77-84
Author(s):  
Gergely Simon ◽  
Marco A. B. Andrade ◽  
Marc P. Y. Desmulliez ◽  
Mathis O. Riehle ◽  
Anne L. Bernassau
Keyword(s):  
Acoustic Waves ◽  
Acoustic Radiation ◽  
Surface Acoustic Waves ◽  
Radiation Force ◽  
Disease Diagnosis ◽  
Target Cells ◽  
Periodic Signal ◽  
Label Free ◽  
Non Invasive ◽  
Sorting Technique

Sorting specific target entities from sample mixtures is commonly used in many macroscale laboratory processing, such as disease diagnosis or treatment. Downscaling of sorting systems enables less laboratory space and fewer quantities of sample and reagent. Such lab-on-a-chip devices can perform separation functions using passive or active sorting methods. Such a method, acoustic sorting, when used in microfluidics, offers contactless, label-free, non-invasive manipulation of target cells or particles and is therefore the topic of active current research. Our phase-modulated sorting technique complements traditional time-of-flight techniques and offers higher sensitivity separation using a periodic signal. By cycling of this periodic signal, the target entities are gradually displaced compared to the background debris, thereby achieving sorting. In this paper, we extend the knowledge on phase-modulated sorting techniques. Firstly, using numerical simulations, we confirm the sorting role of our proposed primary acoustic radiation force within surface wave devices. Secondly, a threefold agreement between analytical, numerical and experimental sorting trajectories is presented.

Generalized Method to Analyze Acoustomechanical Stability of Soft Materials

2016 ◽  
Vol 83 (7) ◽  
Author(s):  
Fengxian Xin ◽  
Tianjian Lu
Keyword(s):  
Wave Propagation ◽  
Acoustic Waves ◽  
Acoustic Radiation ◽  
Radiation Force ◽  
Soft Materials ◽  
Theoretical Method ◽  
Positive Definite ◽  
Soft Material ◽  
Acoustic Radiation Force ◽  
Tangential Stiffness

Acoustic radiation force generated by two counterpropagating acoustic waves in a thin layer of soft material can induce large deformation, and hence can be applied to design acoustomechanical actuators. Owing to the sensitivity of wave propagation to material geometry, the change of layer thickness may enhance wave propagation and acoustic radiation force, causing a jumping larger deformation, i.e., snap-through instability. Built upon the basis of strong elliptic condition, we develop a generalized theoretical method to evaluate the acoustomechanical stability of soft material actuators. We demonstrate that acoustomechanical instability occurs when the true tangential stiffness matrix ceases to be positive definite. Our results show that prestresses can not only enhance significantly the acoustomechanical stability of the soft material layer but also amplify its actuation stretch in thickness direction.

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