scholarly journals Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue

2010 ◽  
Vol 35 (12) ◽  
pp. 2067 ◽  
Author(s):  
Zijian Guo ◽  
Song Hu ◽  
Lihong V. Wang
2011 ◽  
Author(s):  
Zijian Guo ◽  
Song Hu ◽  
Christopher P. Favazza ◽  
Todd N. Erpelding ◽  
Ladislav Jankovic ◽  
...  

Author(s):  
Yinhao Pan ◽  
Ningbo Chen ◽  
Liangjian Liu ◽  
Chengbo Liu ◽  
Zhiqiang Xu ◽  
...  

AbstractPhotoacoustic microscopy is an in vivo imaging technology based on the photoacoustic effect. It is widely used in various biomedical studies because it can provide high-resolution images while being label-free, safe, and harmless to biological tissue. Polygon-scanning is an effective scanning method in photoacoustic microscopy that can realize fast imaging of biological tissue with a large field of view. However, in polygon-scanning, fluctuations of the rotating motor speed and the geometric error of the rotating mirror cause image distortions, which seriously affect the photoacoustic-microscopy imaging quality. To improve the image quality of photoacoustic microscopy using polygon-scanning, an image correction method is proposed based on accurate ultrasound positioning. In this method, the photoacoustic and ultrasound imaging data of the sample are simultaneously obtained, and the angle information of each mirror used in the polygon-scanning is extracted from the ultrasonic data to correct the photoacoustic images. Experimental results show that the proposed method can significantly reduce image distortions in photoacoustic microscopy, with the image dislocation offset decreasing from 24.774 to 10.365 μm.


2010 ◽  
Vol 35 (19) ◽  
pp. 3195 ◽  
Author(s):  
Chi Zhang ◽  
Konstantin Maslov ◽  
Lihong V. Wang

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