Integrated Raman spectroscopy and trimodal wide-field imaging techniques for real-time in vivo tissue Raman measurements at endoscopy

2009 ◽  
Vol 34 (6) ◽  
pp. 758 ◽  
Author(s):  
Zhiwei Huang ◽  
Seng Khoon Teh ◽  
Wei Zheng ◽  
Jianhua Mo ◽  
Kan Lin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
Imaging Techniques ◽  
Wide Field ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain

2021 ◽  
Vol 2 (2) ◽  
pp. 100542
Author(s):  
Taiga Takahashi ◽  
Hong Zhang ◽  
Kohei Otomo ◽  
Yosuke Okamura ◽  
Tomomi Nemoto
Keyword(s):  
Mouse Brain ◽  
Wide Field ◽  
Cranial Window ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals Super-resolution endoscopy for real-time wide-field imaging

2015 ◽  
Vol 23 (13) ◽  
pp. 16803 ◽  
Author(s):  
Feifei Wang ◽  
Hok Sum Sam Lai ◽  
Lianqing Liu ◽  
Pan Li ◽  
Haibo Yu ◽  
...  
Keyword(s):  
Real Time ◽  
Super Resolution ◽  
Wide Field ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media

2021 ◽  
Vol 7 (28) ◽  
pp. eaay5496
Author(s):  
Cheng Zheng ◽  
Jong Kang Park ◽  
Murat Yildirim ◽  
Josiah R. Boivin ◽  
Yi Xue ◽  
...  
Keyword(s):  
High Resolution ◽  
Structural Features ◽  
Computational Imaging ◽  
Tissue Imaging ◽  
Wide Field ◽  
Scattering Media ◽  
Temporal Focusing ◽  
Field Imaging ◽  
Wide Field Imaging

Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found successes in optically cleared tissues and at shallower depths, but the scattering of emission photons in thick turbid samples severely degrades image quality at the camera. To address this challenge, we introduce a novel technique called De-scattering with Excitation Patterning or “DEEP,” which uses patterned nonlinear excitation followed by computational imaging–assisted wide-field detection. Multiphoton temporal focusing allows high-resolution excitation patterns to be projected deep inside specimen at multiple scattering lengths due to the use of long wavelength light. Computational reconstruction allows high-resolution structural features to be reconstructed from tens to hundreds of DEEP images instead of millions of point-scanning measurements.

scholarly journals Direct wide-field radio imaging in real-time at high time resolution using antenna electric fields

2019 ◽  
Vol 491 (1) ◽  
pp. 254-263
Author(s):  
James Kent ◽  
Adam P Beardsley ◽  
Landman Bester ◽  
Steve F Gull ◽  
Bojan Nikolic ◽  
...  
Keyword(s):  
Electric Field ◽  
Real Time ◽  
Electric Fields ◽  
Imaging Method ◽  
Wide Field ◽  
Direct Imaging ◽  
Computational Performance ◽  
Radio Imaging ◽  
Field Imaging ◽  
Wide Field Imaging

ABSTRACT The recent demonstration of a real-time direct imaging radio interferometry correlator represents a new capability in radio astronomy. However, wide-field imaging with this method is challenging since wide-field effects and array non-coplanarity degrade image quality if not compensated for. Here, we present an alternative direct imaging correlation strategy using a direct Fourier transform (DFT), modelled as a linear operator facilitating a matrix multiplication between the DFT matrix and a vector of the electric fields from each antenna. This offers perfect correction for wide field and non-coplanarity effects. When implemented with data from the Long Wavelength Array (LWA), it offers comparable computational performance to previously demonstrated direct imaging techniques, despite having a theoretically higher floating point cost. It also has additional benefits, such as imaging sparse arrays and control over which sky coordinates are imaged, allowing variable pixel placement across an image. It is in practice a highly flexible and efficient method of direct radio imaging when implemented on suitable arrays. A functioning electric field direct imaging architecture using the DFT is presented, alongside an exploration of techniques for wide-field imaging similar to those in visibility-based imaging, and an explanation of why they do not fit well to imaging directly with the digitized electric field data. The DFT imaging method is demonstrated on real data from the LWA telescope, alongside a detailed performance analysis, as well as an exploration of its applicability to other arrays.

Research on Wide-field Imaging Techniques for Low-frequency Radio Arraytwo

2018 ◽  
Vol 42 (4) ◽  
pp. 626-651 ◽  
Author(s):  
Lao Bao-qiang ◽  
An Tao ◽  
Chen Xiao ◽  
Wu Xiao-cong ◽  
Lu Yang
Keyword(s):  
Imaging Techniques ◽  
Low Frequency ◽  
Wide Field ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals Quantitative Wide-Field Imaging Techniques for Fluorescence Guided Neurosurgery

2019 ◽  
Vol 6 ◽  
Author(s):  
Pablo A. Valdes ◽  
Parikshit Juvekar ◽  
Nathalie Y. R. Agar ◽  
Sylvain Gioux ◽  
Alexandra J. Golby
Keyword(s):  
Imaging Techniques ◽  
Wide Field ◽  
Field Imaging ◽  
Wide Field Imaging

Update Diabetische Retinopathie

2020 ◽  
Vol 6 (4) ◽  
pp. 188-191
Author(s):  
Ines Boussaid ◽  
Albert J. Augustin
Keyword(s):  
Structural Changes ◽  
Imaging Techniques ◽  
Clinical Signs ◽  
Microvascular Disease ◽  
Wide Field ◽  
Single Shot ◽  
Fundus Fluorescein Angiography ◽  
Fluid Extravasation ◽  
Field Imaging ◽  
Wide Field Imaging

In the last decades, significant changes have been taking place regarding the pathogenesis of diabetic retinopathy (DR) and the complex mechanisms that eventually lead to the various manifestations of the disease, including diabetic macular edema (DME). DR was first considered a pure microvascular disease, due to the evident capillary structural changes (microaneurysms), fluid extravasation, and lipid exudation. With the advent of fundus fluorescein angiography, the concept of ischemia and the correlation between peripheral nonperfusion and neovascularization has been introduced, which was eventually followed by the advent of new therapeutic strategies, such as peripheral photocoagulation. Nowadays, thanks to more advanced imaging techniques, namely optical coherence tomography (OCT), OCT angiography, and wide-field imaging (imaging up to 200° of the retina in a single shot), it became clear that other elements participate in the occurrence of DR and DME, including inflammation and neurodegeneration. In the future, integration of standard investigations with new diagnostic devices would allow the prompt recognition of DR even before clinical signs of the disease are ophthalmoscopically evident, and the development of personalized treatment for both retinopathy and DME will be available.

scholarly journals Simultaneous fluorescence imaging of tilted focal planes at two depths in thick neural tissue: Implementation with remote focus in a widefield electrophysiological microscope

2019 ◽  
Author(s):  
Bruno Lagarde ◽  
Noah Russell ◽  
Elric Esposito ◽  
Laura Desban ◽  
Claire Wyart ◽  
...  
Keyword(s):  
Brain Slice ◽  
Optical Axis ◽  
Image Plane ◽  
Wide Field ◽  
Single Image ◽  
Time Required ◽  
Field Imaging ◽  
Wide Field Imaging ◽  
Neuronal Compartments

AbstractWide-field imaging conventionally results in a single image plane oriented perpendicular to the optical axis. However, in brain slice or in vivo recording, neuronal or circuit morphologies lie in arbitrarily tilted planes. Consequently the spatiotemporal advantages of wide-field non-scanned imaging are lost because of the time required for stepwise focal readjustments to view an entire neuron or network. We describe an application of remote focus that views simultaneously two planes separated by up to 100 µm, each with variable tilt from the conventional image plane. This permits fluorescence detection of ion fluxes or membrane potential across neuronal compartments and their correlation with electrical activity. Further, two fluorophores can be viewed simultaneously in each plane.We show (i) neuronal images tilted to optimise simultaneous aquisition of somatic, dendritic and axonal compartments; (ii) networks viewed simultaneously at 2 depths separated by up to 100 µm, (iii) widefield imaging at 30 Hz of Gcamp5 fluorescence during spontaneous spiking in motoneuron layers of zebrafish spinal cord separated by 30-40 microns.

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