Fluorescence lifetime imaging with picosecond resolution for biomedical applications

1998 ◽  
Vol 23 (10) ◽  
pp. 810 ◽  
Author(s):  
K. Dowling ◽  
M. J. Dayel ◽  
M. J. Lever ◽  
P. M. W. French ◽  
J. D. Hares ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Biomedical Applications ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Ultra-fast fit-free analysis of complex fluorescence lifetime imaging via deep learning

2019 ◽  
Author(s):  
Jason T. Smith ◽  
Ruoyang Yao ◽  
Nattawut Sinsuebphon ◽  
Alena Rudkouskaya ◽  
Joseph Mazurkiewicz ◽  
...  
Keyword(s):  
Deep Learning ◽  
Fluorescence Lifetime ◽  
Biomedical Applications ◽  
Quantitative Information ◽  
Clinical Settings ◽  
Fluorescence Lifetime Imaging ◽  
Complex Data ◽  
Lifetime Imaging ◽  
Lifetime Studies ◽  
Quantities Of Interest

AbstractFluorescence lifetime imaging (FLI) provides unique quantitative information in biomedical and molecular biology studies, but relies on complex data fitting techniques to derive the quantities of interest. Herein, we propose a novel fit-free approach in FLI image formation that is based on Deep Learning (DL) to quantify complex fluorescence decays simultaneously over a whole image and at ultra-fast speeds. Our deep neural network (DNN), named FLI-Net, is designed and model-based trained to provide all lifetime-based parameters that are typically employed in the field. We demonstrate the accuracy and generalizability of FLI-Net by performing quantitative microscopic and preclinical experimental lifetime-based studies across the visible and NIR spectra, as well as across the two main data acquisition technologies. Our results demonstrate that FLI-Net is well suited to quantify complex fluorescence lifetimes, accurately, in real time in cells and intact animals without any parameter settings. Hence, it paves the way to reproducible and quantitative lifetime studies at unprecedented speeds, for improved dissemination and impact of FLI in many important biomedical applications, especially in clinical settings.

Biomedical Applications of Fluorescence Lifetime Imaging

2002 ◽  
Vol 13 (11) ◽  
pp. 26 ◽  
Author(s):  
Dan Elson ◽  
Stephen Webb ◽  
Jan Siegel ◽  
Klaus Suhling ◽  
Dan Davis ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Biomedical Applications ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

High resolution time-domain fluorescence lifetime imaging for biomedical applications

1999 ◽  
Vol 46 (2) ◽  
pp. 199-209 ◽  
Author(s):  
K. Dowling ◽  
M. J. Dayel ◽  
S. C. W. Hyde ◽  
P. M. W. French ◽  
M. J. Lever ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Lifetime ◽  
Time Domain ◽  
Biomedical Applications ◽  
Fluorescence Lifetime Imaging ◽  
Resolution Time ◽  
Lifetime Imaging

Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging

2021 ◽  
Author(s):  
Lorenzo Scipioni ◽  
Alessandro Rossetta ◽  
Giulia Tedeschi ◽  
Enrico Gratton
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
New Paradigm ◽  
Lifetime Imaging

Interrogation of the liver during regeneration by fluorescence lifetime imaging and mass spectrometry

2021 ◽  
pp. 1-1
Author(s):  
Svetlana Rodimova ◽  
Daria Kuznetsova ◽  
Nikolai Bobrov ◽  
Alexander Gulin ◽  
Dmitry Reunov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging
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