scholarly journals ISM-assisted Tomographic STED Microscopy

2021 ◽  
Author(s):  
Julia Kratz ◽  
Claudia Geisler ◽  
Alexander Egner
Keyword(s):  
Sted Microscopy

scholarly journals Super-resolution STED microscopy in live brain tissue

2021 ◽  
pp. 105420
Author(s):  
Stefano Calovi ◽  
Federico N. Soria ◽  
Jan Tønnesen
Keyword(s):  
Brain Tissue ◽  
Super Resolution ◽  
Sted Microscopy

scholarly journals Dynamic nanoscale morphology of the ER surveyed by STED microscopy

2018 ◽  
Vol 218 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Lena K. Schroeder ◽  
Andrew E.S. Barentine ◽  
Holly Merta ◽  
Sarah Schweighofer ◽  
Yongdeng Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
Stimulated Emission ◽  
Membrane Structures ◽  
Spatiotemporal Resolution ◽  
Sted Microscopy ◽  
Superresolution Microscopy ◽  
Structure And Dynamics ◽  
First Time ◽  
Nanoscale Dynamics ◽  
Conventional Light Microscopy

The endoplasmic reticulum (ER) is composed of interconnected membrane sheets and tubules. Superresolution microscopy recently revealed densely packed, rapidly moving ER tubules mistaken for sheets by conventional light microscopy, highlighting the importance of revisiting classical views of ER structure with high spatiotemporal resolution in living cells. In this study, we use live-cell stimulated emission depletion (STED) microscopy to survey the architecture of the ER at 50-nm resolution. We determine the nanoscale dimensions of ER tubules and sheets for the first time in living cells. We demonstrate that ER sheets contain highly dynamic, subdiffraction-sized holes, which we call nanoholes, that coexist with uniform sheet regions. Reticulon family members localize to curved edges of holes within sheets and are required for their formation. The luminal tether Climp63 and microtubule cytoskeleton modulate their nanoscale dynamics and organization. Thus, by providing the first quantitative analysis of ER membrane structure and dynamics at the nanoscale, our work reveals that the ER in living cells is not limited to uniform sheets and tubules; instead, we suggest the ER contains a continuum of membrane structures that includes dynamic nanoholes in sheets as well as clustered tubules.

scholarly journals Cover Picture: STED Microscopy and its Applications: New Insights into Cellular Processes on the Nanoscale (ChemPhysChem 8/2012)

ChemPhysChem ◽  
2012 ◽  
Vol 13 (8) ◽  
pp. 1965-1965
Author(s):  
Tobias Müller ◽  
Christian Schumann ◽  
Annette Kraegeloh
Keyword(s):  
Sted Microscopy ◽  
Cellular Processes

scholarly journals Features of endogenous cardiomyocyte chromatin revealed by super‐resolution STED microscopy

2012 ◽  
Vol 53 (4) ◽  
pp. 552-558 ◽  
Author(s):  
Scherise Mitchell-Jordan ◽  
Haodong Chen ◽  
Sarah Franklin ◽  
Enrico Stefani ◽  
Laurent A. Bentolila ◽  
...  
Keyword(s):  
Super Resolution ◽  
Sted Microscopy

scholarly journals Subcellular orchestration of alpha-synuclein variants in Parkinson’s disease brains revealed by 3D multicolor STED microscopy

2018 ◽  
Author(s):  
Tim E. Moors ◽  
Christina A. Maat ◽  
Daniel Niedieker ◽  
Daniel Mona ◽  
Dennis Petersen ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Bodies ◽  
Distribution Patterns ◽  
Alpha Synuclein ◽  
Label Free ◽  
Post Translational Modifications ◽  
Sted Microscopy ◽  
C Terminus

AbstractPost-translational modifications of alpha-synuclein (aSyn), particularly phosphorylation at Serine 129 (Ser129-p) and truncation of its C-terminus (CTT), have been implicated in Parkinson’s disease (PD) pathology. To gain more insight in the relevance of Ser129-p and CTT aSyn under physiological and pathological conditions, we investigated their subcellular distribution patterns in normal aged and PD brains using highly-selective antibodies in combination with 3D multicolor STED microscopy. We show that CTT aSyn localizes in mitochondria in PD patients and controls, whereas the organization of Ser129-p in a cytoplasmic network is strongly associated with pathology. Nigral Lewy bodies show an onion skin-like architecture, with a structured framework of Ser129-p aSyn and neurofilaments encapsulating CTT aSyn in their core, which displayed high content of proteins and lipids by label-free CARS microscopy. The subcellular phenotypes of antibody-labeled pathology identified in this study provide evidence for a crucial role of Ser129-p aSyn in Lewy body formation.

Three dimensional live-cell STED microscopy at increased depth using a water immersion objective

2018 ◽  
Vol 89 (5) ◽  
pp. 053701 ◽  
Author(s):  
Jörn Heine ◽  
Christian A. Wurm ◽  
Jan Keller-Findeisen ◽  
Andreas Schönle ◽  
Benjamin Harke ◽  
...  
Keyword(s):  
Water Immersion ◽  
Three Dimensional ◽  
Live Cell ◽  
Sted Microscopy ◽  
Water Immersion Objective

scholarly journals In vivo STED microscopy visualizes PSD95 sub-structures and morphological changes over several hours in the mouse visual cortex

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Waja Wegner ◽  
Alexander C. Mott ◽  
Seth G. N. Grant ◽  
Heinz Steffens ◽  
Katrin I. Willig
Keyword(s):  
Visual Cortex ◽  
Morphological Changes ◽  
Sted Microscopy ◽  
Mouse Visual Cortex

FCS in STED Microscopy

2013 ◽  
pp. 1-38 ◽  
Author(s):  
Veronika Mueller ◽  
Alf Honigmann ◽  
Christian Ringemann ◽  
Rebecca Medda ◽  
Günter Schwarzmann ◽  
...  
Keyword(s):  
Sted Microscopy
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