scholarly journals Ptychography-based high-throughput lensless on-chip microscopy via incremental proximal algorithms

2021 ◽  
Author(s):  
Huang Yue ◽  
Shaowei Jiang ◽  
Ruihai Wang ◽  
Pengming Song ◽  
Jian Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
Proximal Algorithms ◽  
On Chip

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Nan Xiang ◽  
Zhonghua Ni
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Malignant Tumor ◽  
Large Volume ◽  
Concentration Factor ◽  
Pleural Effusions ◽  
Inertial Microfluidics ◽  
Low Concentration ◽  
Malignant Pleural Effusions ◽  
On Chip

On-chip concentration of rare malignant tumor cells (MTCs) in malignant pleural effusions (MPEs) with a large volume is challenging. Previous microfluidic concentrators suffer from a low concentration factor (CF) and...

scholarly journals High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging

Lab on a Chip ◽  
2012 ◽  
Vol 12 (23) ◽  
pp. 4968 ◽  
Author(s):  
Serap Altay Arpali ◽  
Caglar Arpali ◽  
Ahmet F. Coskun ◽  
Hsin-Hao Chiang ◽  
Aydogan Ozcan
Keyword(s):  
High Throughput ◽  
Whole Blood ◽  
High Throughput Screening ◽  
Structured Illumination ◽  
On Chip

scholarly journals Calcinated gold nanoparticle arrays for on-chip, multiplexed and matrix-free mass spectrometric analysis of peptides and small molecules

Nanoscale ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 1665-1675 ◽  
Author(s):  
Samuel S. Hinman ◽  
Chih-Yuan Chen ◽  
Jicheng Duan ◽  
Quan Cheng
Keyword(s):  
Small Molecules ◽  
Gold Nanoparticle ◽  
High Throughput ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Nanoparticle Arrays ◽  
Silicate Coating ◽  
On Chip ◽  
Matrix Free

Patterned gold nanoparticle microarrays, with a nanoscale silicate coating, have been developed for on-chip, high-throughput mass spectrometric analysis of biomolecules.

scholarly journals High-throughput fabrication and calibration of compact high-sensitivity plasmonic lab-on-chip for biosensing

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
E. Gazzola ◽  
A. Pozzato ◽  
G. Ruffato ◽  
E. Sovernigo ◽  
A. Sonato
Keyword(s):  
High Throughput ◽  
High Sensitivity ◽  
Controlled Environment ◽  
Biological Applications ◽  
Environment Monitoring ◽  
Lab On Chip ◽  
Sensing Platforms ◽  
Multiple Detection ◽  
On Chip ◽  
Biological Environment

AbstractSurface plasmon resonance biosensors have recently known a rapid diffusion in the biological field and a large variety of sensor configurations is currently available. Biological applications are increasingly demanding sensor miniaturization, multiple detection in parallel, temperature-controlled environment and high sensitivity. Indeed, versatile and tunable sensing platforms, together with an accurate biological environment monitoring, could improve the realization of custom biosensing devices applicable to different biological reactions. Here we propose a smart and high throughput fabrication protocol for the realization of a custommicrofluidic plasmonic biochip that could be easily tuned and modified to address different biological applications. The sensor chip here presented shows a high sensing capability, monitored by an accurate signal calibration in the presence of concentration and temperature variation.

scholarly journals Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis

2016 ◽  
Vol 113 (52) ◽  
pp. 14915-14920 ◽  
Author(s):  
Yih Yang Chen ◽  
Pamuditha N. Silva ◽  
Abdullah Muhammad Syed ◽  
Shrey Sindhwani ◽  
Jonathan V. Rocheleau ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Three Dimensional ◽  
Microfluidic System ◽  
High Throughput Drug Screening ◽  
Image Analysis Algorithm ◽  
Screening Assays ◽  
On Chip ◽  
Tissue Models

On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the exchange of interstitial fluids by 567-fold, which increased the rate of removal of optically scattering lipid molecules from the cross-linked tissue. Our enhanced clearing process allowed us to fluorescently image and map the segregation and compartmentalization of different cells during the formation of tumor spheroids, and to track the degradation of vasculature over time within extracted murine pancreatic islets in static culture, which may have implications on the efficacy of beta-cell transplantation treatments for type 1 diabetes. We further developed an image analysis algorithm that automates the analysis of the vasculature connectivity, volume, and cellular spatial distribution of the intact tissue. Our technique allows whole tissue analysis in microfluidic systems, and has implications in the development of organ-on-a-chip systems, high-throughput drug screening devices, and in regenerative medicine.

scholarly journals Handheld Microflow Cytometer Based on a Motorized Smart Pipette, a Microfluidic Cell Concentrator, and a Miniaturized Fluorescence Microscope

Sensors ◽  
2019 ◽  
Vol 19 (12) ◽  
pp. 2761 ◽  
Author(s):  
Byeongyeon Kim ◽  
Dayoung Kang ◽  
Sungyoung Choi
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Flow Cytometric Analysis ◽  
Sample Volume ◽  
Fluorescence Microscope ◽  
Comprehensive Approach ◽  
Optical Systems ◽  
Simple Protocol ◽  
Microflow Cytometer ◽  
On Chip

Miniaturizing flow cytometry requires a comprehensive approach to redesigning the conventional fluidic and optical systems to have a small footprint and simple usage and to enable rapid cell analysis. Microfluidic methods have addressed some challenges in limiting the realization of microflow cytometry, but most microfluidics-based flow cytometry techniques still rely on bulky equipment (e.g., high-precision syringe pumps and bench-top microscopes). Here, we describe a comprehensive approach that achieves high-throughput white blood cell (WBC) counting in a portable and handheld manner, thereby allowing the complete miniaturization of flow cytometry. Our approach integrates three major components: a motorized smart pipette for accurate volume metering and controllable liquid pumping, a microfluidic cell concentrator for target cell enrichment, and a miniaturized fluorescence microscope for portable flow cytometric analysis. We first validated the capability of each component by precisely metering various fluid samples and controlling flow rates in a range from 219.5 to 840.5 μL/min, achieving high sample-volume reduction via on-chip WBC enrichment, and successfully counting single WBCs flowing through a region of interrogation. We synergistically combined the three major components to create a handheld, integrated microflow cytometer and operated it with a simple protocol of drawing up a blood sample via pipetting and injecting the sample into the microfluidic concentrator by powering the motorized smart pipette. We then demonstrated the utility of the microflow cytometer as a quality control means for leukoreduced blood products, quantitatively analyzing residual WBCs (rWBCs) in blood samples present at concentrations as low as 0.1 rWBCs/μL. These portable, controllable, high-throughput, and quantitative microflow cytometric technologies provide promising ways of miniaturizing flow cytometry.

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