scholarly journals Improved reconstruction method for phase stepping data with stepping errors and dose fluctuations

2020 ◽  
Vol 28 (11) ◽  
pp. 16363 ◽  
Author(s):  
Koh Hashimoto ◽  
Hidekazu Takano ◽  
Atsuhi Momose
Keyword(s):  
Reconstruction Method ◽  
Phase Stepping

Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes

1987 ◽  
Vol 45 ◽  
pp. 936-937
Author(s):  
Neng-Yu Zhang ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
Tom Obrig ◽  
Joachim Frank
Keyword(s):  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Uranyl Acetate ◽  
Reconstruction Method ◽  
Single Exposure ◽  
Electron Dose ◽  
Ribosomal Subunits ◽  
40S Ribosomal Subunit ◽  
Dimensional Reconstruction ◽  
Rabbit Reticulocytes

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.

Development of Waveform Reconstruction Method with Current Transformer Saturation

2009 ◽  
Vol 129 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Shoichi Urano ◽  
Takeshi Yamada ◽  
Yoshifumi Ooura ◽  
Youheng Xu ◽  
Yasutaka Yamaguchi ◽  
...  
Keyword(s):  
Current Transformer ◽  
Reconstruction Method

3D Scene Reconstruction Method in Relative Coordinates with Two Images from Non-Calibrated Video Cameras

2020 ◽  
Vol 25 (3) ◽  
pp. 265-276
Author(s):  
K.M. Shepilova ◽  
◽  
A.V. Sotnikov ◽  
A.V. Shipatov ◽  
Yu.V. Savchenko ◽  
...  
Keyword(s):  
Scene Reconstruction ◽  
Reconstruction Method ◽  
Video Cameras ◽  
Relative Coordinates ◽  
3D Scene ◽  
3D Scene Reconstruction

HIGH-RESOLUTION SPECTRAL RECONSTRUCTION: METHOD AND APPLICATIONS FOR CONTAMINATION MEASUREMENT, DIGITAL SAMPLING, AND CONTINUITY ASSESSMENT

2020 ◽  
Author(s):  
Christopher Michael Jones ◽  
◽  
Yngve B Johansen ◽  
Artur Kotwicki ◽  
Cameron Rekully ◽  
...  
Keyword(s):  
High Resolution ◽  
Reconstruction Method ◽  
Digital Sampling ◽  
Spectral Reconstruction

Social anxiety is associated with similar emotional impairments during digital and face-to-face communication in daily life

2020 ◽  
Author(s):  
James Doorley ◽  
Kristina Volgenau ◽  
Kerry Kelso ◽  
Todd Barrett Kashdan ◽  
Alexander J. Shackman
Keyword(s):  
Social Anxiety ◽  
Digital Communication ◽  
Instant Messaging ◽  
Online Communication ◽  
Medium Study ◽  
Reconstruction Method ◽  
Communication Behaviors ◽  
Face To Face ◽  
Emotional Impairments ◽  
Emotional Safety

Background:Retrospective studies have found that people with elevated social anxiety (SA) show a preference for digital/online communication, which may be due to perceptions of enhanced emotional safety. Whether these preferences for/benefits of digital compared to face-to-face communication manifest in the real world has yet to be explored. Methods: We recruited samples of college students (N = 125) and community adults (N = 303) with varying levels of SA, sampled their emotions during digital and face-to-face communication using ecological momentary assessment (EMA) (Study 1) and a day reconstruction method (DRM) (Study 2), and preregistered our hypotheses (https://osf.io/e4y7x/). Results: Results from both studies showed that SA did not predict the likelihood of engaging in digital compared to face-to-face communication, and SA was associated with less positive and more negative emotions regardless of communication medium. Study 2 also showed that whether digital communication was synchronous (e.g., in real time via phone/video chat) or asynchronous (e.g., texting/instant messaging) did not impact the association between SA and emotions. Limitations: EMA and DRM methods, despite their many advantages, may be suboptimal for assessing the occurrence of digital communication behaviors relative to more objective methods (e.g., passively collecting smartphone communication data). Using event-contingent responding may have also yielded more reports of digital communication, thus strengthening our power to detect small, cross-level interaction effects. Conclusions:These results challenge beliefs that digital/online communication provides a source of emotional safety for people with elevated SA and suggests a greater need to address SA-related emotional impairments across digital communication platforms.

Выбор методов реконструкции самотечных канализационных сетей

2019 ◽  
pp. 35-41
Author(s):  
Viktor, Vasil’ev ◽  
G. Morozov ◽  
S. Zhukov
Keyword(s):  
System Approach ◽  
Block Diagram ◽  
Essential Element ◽  
The Self ◽  
Operating Costs ◽  
Reconstruction Method ◽  
Existing Structures ◽  
Sewage System ◽  
Automation Control ◽  
Sewer Networks

Реконструкция канализационных сетей становится важнейшим элементом поддержания всей системы канализации в рабочем состоянии за счет минимизации эксплуатационных затрат, увеличения срока службы трубопроводов, повышения автономности в работе системы с помощью современных средств автоматизации, контроля и анализа. Для того чтобы максимально продлить срок службы трубопроводов и не причинить вреда параллельно проложенным инженерным сетям, необходимо грамотно подбирать методы и способы реконструкции сетей, основываясь на опыте и анализе работы существующих. Рассмотрены и классифицированы основные методы реконструкции и ремонта трубопроводов. Отмечена необходимость в системной реконструкции. Разработана блок-схема, позволяющая выбрать метод реконструкции в зависимости от характера разрушения трубопровода на основании телеметрического обследования. Приведены примеры ее применения на реальных объектах. Статья написана на основе анализа технической литературы и опыта эксплуатации и проектирования канализационных сетей в Санкт-Петербурге.Rehabilitation of sewer networks becomes an essential element in maintaining the entire sewage system in running order by minimizing the operating costs, increasing the life of pipelines, increasing the self-sustainability of the system using advanced automation, control and analysis tools. In order to maximize the life of pipelines and eliminate damaging parallel laid utility networks, it is necessary to correctly choose the methods of networks rehabilitation based on the experience and analysis of the existing ones. The main methods of repair and rehabilitation of pipelines are considered and classified. The necessity for system approach to the reconstruction is noted. A block diagram has been developed that provides for choosing a reconstruction method depending on the nature of the pipeline destruction and based on the telemetry inspection. Examples of its application in the existing structures are given. The article is written on the basis of an analysis of the technical publications and experience of operating and designing sewer networks in St. Petersburg.

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