scholarly journals Label-free rapid identification of tumor cells and blood cells with silver film SERS substrate

2018 ◽  
Vol 26 (25) ◽  
pp. 33044 ◽  
Author(s):  
Y. J. Zhang ◽  
Q. Y. Zeng ◽  
L. F. Li ◽  
M. N. Qi ◽  
Q. C. Qi ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Blood Cells ◽  
Silver Film ◽  
Rapid Identification ◽  
Sers Substrate ◽  
Label Free

Classification of blood cells and tumor cells using label-free ultrasound and photoacoustics

2015 ◽  
Vol 87 (8) ◽  
pp. 741-749 ◽  
Author(s):  
Eric M. Strohm ◽  
Michael C. Kolios
Keyword(s):  
Tumor Cells ◽  
Blood Cells ◽  
Label Free

On the Design of a DLD-DEP Device for Separation of Circulating Tumor Cells in Blood

2020 ◽  
Author(s):  
Mehdi Rahmati ◽  
Xiaolin Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Progression ◽  
High Throughput ◽  
Blood Cells ◽  
Lateral Displacement ◽  
Uniform Electric Field ◽  
Label Free ◽  
Particle Tracing ◽  
Key Factor

Abstract Circulating Tumor Cells (CTCs), which migrate from original sites in a body to distant organs through blood, are a key factor in cancer detection. Emerging Label-free techniques owing to their inherent advantage to preserve characteristics of sorted cells and low consumption of samples can be promising to the prediction of cancer progression and metastasis research. Deterministic Lateral Displacement (DLD) is one of the label-free separation techniques employing a specific arrangement of micro-posts for continuous separation of suspended cells in a buffer based on the size of cells. Separation based solely on size is challenging since the size distributions of CTCs might overlap with those of normal blood cells. To address this problem, DLD can be combined with dielectrophoresis (DEP) technique which is the phenomenon of particle movement in a non-uniform electric field owing to the polarization effect. Although, DLD devices employ the laminar flow in low Reynolds number (Re) fluid flow due to predictability of such flow regimes, they should be improved to work in higher Re flow regime so as to attain high throughput devices. In this paper, a particle tracing simulation is developed to study the effects of different post shapes, shift fraction of micropost arrays, and dielectrophoresis forces on separation of CTCs from peripheral blood cells. Our numerical model and results provide a groundwork for design and fabrication of high-throughput DLD-DEP devices for improvement of CTC separation.

Acoustofluidic, Label-Free Separation and Simultaneous Concentration of Rare Tumor Cells from White Blood Cells

2015 ◽  
Vol 87 (18) ◽  
pp. 9322-9328 ◽  
Author(s):  
Maria Antfolk ◽  
Cecilia Magnusson ◽  
Per Augustsson ◽  
Hans Lilja ◽  
Thomas Laurell
Keyword(s):  
Tumor Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Rare Tumor ◽  
Label Free

Emerging Microfluidic Technologies for the Detection of Circulating Tumor Cells and Fetal Nucleated Red Blood Cells

2021 ◽  
Vol 4 (2) ◽  
pp. 1140-1155
Author(s):  
Xiaoyun Wei ◽  
Keke Chen ◽  
Shishang Guo ◽  
Wei Liu ◽  
Xing-Zhong Zhao
Keyword(s):  
Red Blood Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Blood Cells ◽  
Nucleated Red Blood Cells

scholarly journals Label-free fingerprinting of tumor cells in bulk flow using inline digital holographic microscopy

2017 ◽  
Vol 8 (2) ◽  
pp. 536 ◽  
Author(s):  
Dhananjay Kumar Singh ◽  
Caroline C. Ahrens ◽  
Wei Li ◽  
Siva A. Vanapalli
Keyword(s):  
Tumor Cells ◽  
Bulk Flow ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy

scholarly journals 56P Single circulating tumor cells RNA profiling by label-free enrichment and active single cell selection on an integrated fluidic system

2016 ◽  
Vol 27 ◽  
pp. ix15-ix16
Author(s):  
Y.F. Lee ◽  
N. Ramalingam ◽  
L. Szpankowski ◽  
A. Leyrat ◽  
N.D. Angeles ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Selection ◽  
Label Free ◽  
Rna Profiling ◽  
Free Enrichment

scholarly journals Design of Inverted Nano-Cone Arrayed SERS Substrate for Rapid Detection of Pathogens

2021 ◽  
Vol 11 (17) ◽  
pp. 8067
Author(s):  
Zixun Jia ◽  
Sarah Asiri ◽  
Asma Elsharif ◽  
Widyan Alamoudi ◽  
Ebtesam Al-Suhaimi ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Close Contact ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Substrate ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Order Of Magnitude ◽  
The Right ◽  
Pathogen Diagnosis

Rapid detection of bacteria is a very critical and important part of infectious disease treatment. Sepsis kills more than 25 percent of its victims, resulting in as many as half of all deaths in hospitals before identifying the pathogen for patients to get the right treatment. Raman spectroscopy is a promising candidate in pathogen diagnosis given its fast and label-free nature, only if the concentration of the pathogen is high enough to provide reasonable sensitivity. This work reports a new design of surface-enhanced Raman spectroscopy (SERS) substrate which will provide high enough sensitivity and fast and close contact of the target structure to the optical hot spots for immunomagnetic capturing-based bacteria-concentrating technique. The substrate uses inverted nanocone structure arrays made of transparent PDMS (Polydimethylsiloxane) to funnel the light from the bottom to the top of the cones where plasmonic gold nanorods are located. A high reflective and low loss layer is deposited on the outer surface of the cone. Given the geometry of cones, photons are multi-reflected by the outer layer and thus the number density of photons at hotspots increases by an order of magnitude, which could be high enough to detect immunomagnetically densified bacteria.

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