scholarly journals On-line analysis of algae in water by discrete three-dimensional fluorescence spectroscopy

2018 ◽  
Vol 26 (6) ◽  
pp. A251 ◽  
Author(s):  
Nanjing Zhao ◽  
Xiaoling Zhang ◽  
Gaofang Yin ◽  
Ruifang Yang ◽  
Li Hu ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Three Dimensional ◽  
On Line ◽  
Line Analysis

Fluorescence spectroscopy as a novel method for on-line analysis of biocatalytic C–C bond formations

2010 ◽  
Vol 66 (1-2) ◽  
pp. 124-129 ◽  
Author(s):  
Selin Kara ◽  
Fabienne Anton ◽  
Dörte Solle ◽  
Markus Neumann ◽  
Bernd Hitzmann ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
On Line ◽  
Novel Method ◽  
Line Analysis

Design and calibration of an IVEM for general 3-dimensional imaging of non-diffracting materials

1992 ◽  
Vol 50 (2) ◽  
pp. 1040-1041
Author(s):  
Neil Rowlands ◽  
Jeff Price ◽  
Michael Kersker ◽  
Seichi Suzuki ◽  
Steve Young ◽  
...  
Keyword(s):  
3D Imaging ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
3 Dimensional ◽  
3D Microstructure ◽  
Image Translation ◽  
Digital Correlation ◽  
On Line ◽  
Mechanical Stage ◽  
Projection Angle

Three-dimensional (3D) microstructure visualization on the electron microscope requires that the sample be tilted to different positions to collect a series of projections. This tilting should be performed rapidly for on-line stereo viewing and precisely for off-line tomographic reconstruction. Usually a projection series is collected using mechanical stage tilt alone. The stereo pairs must be viewed off-line and the 60 to 120 tomographic projections must be aligned with fiduciary markers or digital correlation methods. The delay in viewing stereo pairs and the alignment problems in tomographic reconstruction could be eliminated or improved by tilting the beam if such tilt could be accomplished without image translation.A microscope capable of beam tilt with simultaneous image shift to eliminate tilt-induced translation has been investigated for 3D imaging of thick (1 μm) biologic specimens. By tilting the beam above and through the specimen and bringing it back below the specimen, a brightfield image with a projection angle corresponding to the beam tilt angle can be recorded (Fig. 1a).

scholarly journals Hemp (Cannabis sativa L.) Seed Protein–EGCG Conjugates: Covalent Bonding and Functional Research

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1618
Author(s):  
Xin-Hui Pang ◽  
Yang Yang ◽  
Xin Bian ◽  
Bing Wang ◽  
Li-Kun Ren ◽  
...  
Keyword(s):  
Particle Size ◽  
Fluorescence Spectroscopy ◽  
Cannabis Sativa ◽  
Three Dimensional ◽  
Epigallocatechin Gallate ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Particle Size Data ◽  
Covalently Bonded ◽  
Covalently Linked

In order to make HPI have a wide application prospect in the food industry, we used EGCG to modify HPI. In this study, we prepared different concentrations (1, 2, 3, 4, and 5 mM) of (−)-epigallocatechin gallate (EGCG) covalently linked to HPI and use methods such as particle size analysis, circular dichroism (CD), and three-dimensional fluorescence spectroscopy to study the changes in the structure and functional properties of HPI after being covalently combined with EGCG. The particle size data indicated that the covalent HPI-EGCG complex was larger than native HPI, and the particle size was mainly distributed at about 200 μm. CD and three-dimensional fluorescence spectroscopy analyses showed that the conformation of the protein was changed by conjugation with EGCG. The β-sheet content decreased from 82.79% to 66.67% after EGCG bound to the protein, and the hydrophobic groups inside the protein were exposed, which increased the hydrophobicity of the protein and changed its conformation. After HPI and 1 mM of EGCG were covalently bonded, the solubility and emulsifying properties of the covalent complex were improved compared with native HPI. These results indicated that HPI-EGCG conjugates can be added in some foods.

scholarly journals The Use of Right Angle Fluorescence Spectroscopy to Distinguish the Botanical Origin of Greek Common Honey Varieties

2021 ◽  
Vol 11 (9) ◽  
pp. 4047
Author(s):  
Marinos Xagoraris ◽  
Panagiota-Kyriaki Revelou ◽  
Eleftherios Alissandrakis ◽  
Petros A. Tarantilis ◽  
Christos S. Pappas
Keyword(s):  
Fluorescence Spectroscopy ◽  
External Validation ◽  
Three Dimensional ◽  
Excitation Wavelength ◽  
Botanical Origin ◽  
Linear Discriminant ◽  
Classification Score ◽  
Hydroxybenzoic Acids ◽  
Unifloral Honey ◽  
Novel Method

The standardization of the botanical origin of honey reflects the commercial value and quality of honey. Nowadays, most consumers are looking for a unifloral honey. The aim of the present study was to develop a novel method for honey classification using chemometric models based on phenolic compounds analyzed with right angle fluorescence spectroscopy, coupled with stepwise linear discriminant analysis (LDA). The deconstructed spectrum from three-dimensional-emission excitation matrix (3D-EEM) spectra provided a correct classification score of 94.9% calibration and cross-validation at an excitation wavelength (λex) of 330 nm. Subsequently, a score of 81.4% and 79.7%, respectively, at an excitation wavelength (λex) of 360 nm was achieved. Each chemometric model confirmed its power through the external validation with a score of 82.1% for both. Differentiation could be correlated with hydroxycinnamic and hydroxybenzoic acids, which absorb in this region of the spectrum. Fluorescence spectroscopy constitutes a rapid and sensitive technique, which, when combined with the stepwise algorithm and LDA method, can be used as a reliable and predictive authentication tool for honey. This study indicates that the developed methodology is a promising technique for determination of the botanical origin of common Greek honey varieties. Our long-term ambition is to support producers and suppliers to remain in a competitive national and international market.

Sign in / Sign up

Export Citation Format

Share Document