scholarly journals 910nm femtosecond Nd-doped fiber laser for in vivo two-photon microscopic imaging

2016 ◽  
Vol 24 (15) ◽  
pp. 16544 ◽  
Author(s):  
Bingying Chen ◽  
Tongxiao Jiang ◽  
Weijian Zong ◽  
Liangyi Chen ◽  
Zhigang Zhang ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Microscopic Imaging ◽  
Two Photon

44 nJ, 114 fs Nd-doped fiber laser pulses at 920nm for in vivo two-photon microscopic imaging

CLEO: 2015 ◽  
2015 ◽  
Author(s):  
Bingying Chen ◽  
Tongxiao Jiang ◽  
Weijian Zong ◽  
Fuzeng Niu ◽  
Liangyi Chen ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Laser Pulses ◽  
Microscopic Imaging ◽  
Two Photon

scholarly journals Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser

2016 ◽  
Vol 7 (2) ◽  
pp. 324 ◽  
Author(s):  
Evan P. Perillo ◽  
Justin E. McCracken ◽  
Daniel C. Fernée ◽  
John R. Goldak ◽  
Flor A. Medina ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Low Cost ◽  
Two Photon Microscopy ◽  
Two Photon

In vivo label-free two-photon excitation autofluorescence microscopy of microvasculature using a 520 nm femtosecond fiber laser

2020 ◽  
Vol 45 (10) ◽  
pp. 2704
Author(s):  
Ting Wu ◽  
Jiuling Liao ◽  
Jia Yu ◽  
Yufeng Gao ◽  
Hui Li ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Label Free ◽  
Two Photon ◽  
Photon Excitation ◽  
Femtosecond Fiber Laser ◽  
Two Photon Excitation

Integrated Fluorescent Nanoprobe Design for High‐Speed In Vivo Two‐Photon Microscopic Imaging of Deep‐Brain Vasculature in Mice

2021 ◽  
pp. 2010698
Author(s):  
Minami Takezaki ◽  
Ryosuke Kawakami ◽  
Shozo Onishi ◽  
Yasutaka Suzuki ◽  
Jun Kawamata ◽  
...  
Keyword(s):  
High Speed ◽  
Microscopic Imaging ◽  
Two Photon ◽  
Fluorescent Nanoprobe ◽  
Brain Vasculature ◽  
Deep Brain

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

scholarly journals Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 499
Author(s):  
Tracy W. Liu ◽  
Seth T. Gammon ◽  
David Piwnica-Worms
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Emission Spectra ◽  
Ccd Camera ◽  
Microscopic Imaging ◽  
Signaling Dynamics ◽  
Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

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