scholarly journals Gauging low-dose X-ray phase-contrast imaging at a single and large propagation distance

2016 ◽  
Vol 24 (4) ◽  
pp. 4331 ◽  
Author(s):  
Ralf Hofmann ◽  
Alexander Schober ◽  
Steffen Hahn ◽  
Julian Moosmann ◽  
Jubin Kashef ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Low Dose ◽  
Propagation Distance ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray

scholarly journals A method for high-energy, low-dose mammography using edge illumination x-ray phase-contrast imaging

2016 ◽  
Vol 61 (24) ◽  
pp. 8750-8761 ◽  
Author(s):  
Paul C Diemoz ◽  
Alberto Bravin ◽  
Anikó Sztrókay-Gaul ◽  
Marie Ruat ◽  
Susanne Grandl ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Low Dose ◽  
High Energy ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray

scholarly journals Low-dose and fast grating-based x-ray phase-contrast imaging

2017 ◽  
Vol 56 (09) ◽  
pp. 1 ◽  
Author(s):  
Faiz Wali ◽  
Shenghao Wang ◽  
Huajie Han ◽  
Kun Gao ◽  
Zhao Wu
Keyword(s):  
Phase Contrast ◽  
Low Dose ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray

scholarly journals Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography

IUCrJ ◽  
2020 ◽  
Vol 7 (6) ◽  
pp. 1131-1141
Author(s):  
Isabelle Martiel ◽  
Chia-Ying Huang ◽  
Pablo Villanueva-Perez ◽  
Ezequiel Panepucci ◽  
Shibom Basu ◽  
...  
Keyword(s):  
Data Collection ◽  
Phase Contrast ◽  
Low Dose ◽  
Phase Contrast Imaging ◽  
Cubic Phase ◽  
Macromolecular Crystallography ◽  
Contrast Imaging ◽  
X Ray ◽  
Serial Data

Serial protein crystallography has emerged as a powerful method of data collection on small crystals from challenging targets, such as membrane proteins. Multiple microcrystals need to be located on large and often flat mounts while exposing them to an X-ray dose that is as low as possible. A crystal-prelocation method is demonstrated here using low-dose 2D full-field propagation-based X-ray phase-contrast imaging at the X-ray imaging beamline TOMCAT at the Swiss Light Source (SLS). This imaging step provides microcrystal coordinates for automated serial data collection at a microfocus macromolecular crystallography beamline on samples with an essentially flat geometry. This prelocation method was applied to microcrystals of a soluble protein and a membrane protein, grown in a commonly used double-sandwich in situ crystallization plate. The inner sandwiches of thin plastic film enclosing the microcrystals in lipid cubic phase were flash cooled and imaged at TOMCAT. Based on the obtained crystal coordinates, both still and rotation wedge serial data were collected automatically at the SLS PXI beamline, yielding in both cases a high indexing rate. This workflow can be easily implemented at many synchrotron facilities using existing equipment, or potentially integrated as an online technique in the next-generation macromolecular crystallography beamline, and thus benefit a number of dose-sensitive challenging protein targets.

scholarly journals Large field of view, fast and low dose multimodal phase-contrast imaging at high x-ray energy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Alberto Astolfo ◽  
Marco Endrizzi ◽  
Fabio A. Vittoria ◽  
Paul C. Diemoz ◽  
Benjamin Price ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Low Dose ◽  
Field Of View ◽  
Phase Contrast Imaging ◽  
Large Field ◽  
Contrast Imaging ◽  
X Ray

scholarly journals Low-dose, simple, and fast grating-based X-ray phase-contrast imaging

2010 ◽  
Vol 107 (31) ◽  
pp. 13576-13581 ◽  
Author(s):  
P. Zhu ◽  
K. Zhang ◽  
Z. Wang ◽  
Y. Liu ◽  
X. Liu ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Low Dose ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray

Image Quality Analysis of X-ray Grating Interferometer Using Integrating-bucket Method

2020 ◽  
Vol 64 (2) ◽  
pp. 20503-1-20503-5
Author(s):  
Faiz Wali ◽  
Shenghao Wang ◽  
Ji Li ◽  
Jianheng Huang ◽  
Yaohu Lei ◽  
...  
Keyword(s):  
Image Quality ◽  
Phase Contrast ◽  
Quality Analysis ◽  
Phase Contrast Imaging ◽  
Phase Image ◽  
Contrast Imaging ◽  
High Quality ◽  
Image Quality Analysis ◽  
X Ray ◽  
Grating Interferometer

Abstract Grating-based x-ray phase-contrast imaging has the potential to enhance image quality and provide inner structure details non-destructively. In this work, using grating-based x-ray phase-contrast imaging system and employing integrating-bucket method, the quantitative expressions of signal-to-noise ratios due to photon statistics and mechanical error are analyzed in detail. Photon statistical noise and mechanical error are the main sources affecting the image noise in x-ray grating interferometry. Integrating-bucket method is a new phase extraction method translated to x-ray grating interferometry; hence, its image quality analysis would be of great importance to get high-quality phase image. The authors’ conclusions provide an alternate method to get high-quality refraction signal using grating interferometer, and hence increases applicability of grating interferometry in preclinical and clinical usage.

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