scholarly journals Removing lateral chromatic aberration in bright field optical microscopy

2015 ◽  
Vol 23 (11) ◽  
pp. 14380 ◽  
Author(s):  
Miguel Guzmán-Altamirano ◽  
Braulio Gutiérrez-Medina
Keyword(s):  
Optical Microscopy ◽  
Chromatic Aberration ◽  
Bright Field ◽  
Lateral Chromatic Aberration

scholarly journals Morphological Characteristics of the Sperm of the Peregrine Falcon (Falco peregrinus) during the Reproductive Season

2021 ◽  
Vol 8 (9) ◽  
pp. 169
Author(s):  
Andrea Oliveira ◽  
Felisa Martínez ◽  
Lydia Gil ◽  
Victoria Luño
Keyword(s):  
Optical Microscopy ◽  
Morphological Characteristics ◽  
Reproductive Season ◽  
Peregrine Falcon ◽  
Sperm Cells ◽  
Falco Peregrinus ◽  
Bright Field ◽  
Morphological Abnormalities ◽  
Peregrine Falcons ◽  
Initial Stage

The morphological characteristics of different sperm cells (normal, abnormal, and immature) in the peregrine falcon during the reproductive season were analysed. We also classified the main sperm defects found in semen. Semen samples were collected from mature peregrine falcons via cloacal massage and stained with Diff-Quik stain. The percentages of normal, abnormal, and immature sperm cells were determined by bright-field optical microscopy. The number of normal spermatozoa were greater at the initial stage and subsequently decreased during the middle and later stages of the reproductive season (p < 0.01). In contrast, the percentage of abnormal spermatozoa increased significantly in the middle and end stages of the reproductive season (p < 0.05), whereas the proportion of immature spermatozoa remained stable during the study. Head defects represented the greatest proportion of morphological abnormalities, followed by the defects in the tail and midpiece regions. A small percentage of multiple defects and cytoplasmic droplets were also observed in the falcon spermatozoa. The findings of this study might be important for the development of future conservation protocols for falcon sperm.

scholarly journals TTL Alignment for Excimer Laser Aligner. Real-Time SMART with Correcting Lateral Chromatic Aberration.

1993 ◽  
Vol 59 (1) ◽  
pp. 101-106
Author(s):  
Tatsuhiko HIGASHIKI ◽  
Toru TOJO ◽  
Mitsuo TABATA ◽  
Takeshi NISHIZAKA ◽  
Hisakazu YOSHINO ◽  
...  
Keyword(s):  
Real Time ◽  
Excimer Laser ◽  
Chromatic Aberration ◽  
Lateral Chromatic Aberration

The Use of Tilted Beam Bright-Field Illumination in High Resolution Electron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 566-567 ◽  
Author(s):  
William Krakow
Keyword(s):  
High Resolution ◽  
High Spatial Frequency ◽  
Chromatic Aberration ◽  
High Resolution Electron Microscopy ◽  
Optic Axis ◽  
Lattice Imaging ◽  
Bright Field ◽  
Transmitted Beam ◽  
Transfer Theory ◽  
Optical Transfer

Once it is understood in terms of wave optical transfer theory, tilted beam bright-field illumination can be applied to a variety of high resolution microscope problems which encompass lattice imaging and imaging nonperiodic objects. The tilted beam method offers the advantages of increasing the resolution and allowing both elastically and inelastically scattered electrons to contribute to high spatial frequency detail in a micrograph. In the simplest case, one can demonstrate these effects by the conventional lattice imaging mode using a single diffracted beam and the direct transmitted beam positioned symmetrically on either side of the microscope optic axis. However, the transfer theory of the microscope for tilted beam illumination shows that the cancellation of chromatic aberration occurs not only for the one diffraction direction across the optic axis from the unscattered beam, but also for an entire circle centered about the axis in the back focal plane (BFP) of the microscope.

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