scholarly journals 3D reconstruction of light flux distribution on arbitrary surfaces from 2D multi-photographic images

2010 ◽  
Vol 18 (19) ◽  
pp. 19876 ◽  
Author(s):  
Xueli Chen ◽  
Xinbo Gao ◽  
Duofang Chen ◽  
Xiaopeng Ma ◽  
Xiaohui Zhao ◽  
...  
Author(s):  
A. Cardaci ◽  
A. Versaci ◽  
P. Azzola

Abstract. The creation of three-dimensional models for the cataloguing and documentation of cultural heritage is today an emerging need in the cultural sphere and, above all, for museums. The cultural heritage is still catalogued and documented based on descriptive files assorted of photographic images which, however, fail to outline its spatial richness, possible only through the use of 3D artefacts. The essay aims to propose a methodology of digitalization by low-cost and easy-to-use systems, to be employed even by non-expert survey and photogrammetry’s operators. The case study of the statue of San Nicola da Tolentino, preserved at the Sant’Agostino complex in Bergamo, offered the possibility of a comparison between 3D models acquired with different digitalization tools (professional/action/amateur cameras and smartphone) and processed by several image-based 3D Reconstruction software and methods.


Author(s):  
Jose-Maria Carazo ◽  
I. Benavides ◽  
S. Marco ◽  
J.L. Carrascosa ◽  
E.L. Zapata

Obtaining the three-dimensional (3D) structure of negatively stained biological specimens at a resolution of, typically, 2 - 4 nm is becoming a relatively common practice in an increasing number of laboratories. A combination of new conceptual approaches, new software tools, and faster computers have made this situation possible. However, all these 3D reconstruction processes are quite computer intensive, and the middle term future is full of suggestions entailing an even greater need of computing power. Up to now all published 3D reconstructions in this field have been performed on conventional (sequential) computers, but it is a fact that new parallel computer architectures represent the potential of order-of-magnitude increases in computing power and should, therefore, be considered for their possible application in the most computing intensive tasks.We have studied both shared-memory-based computer architectures, like the BBN Butterfly, and local-memory-based architectures, mainly hypercubes implemented on transputers, where we have used the algorithmic mapping method proposed by Zapata el at. In this work we have developed the basic software tools needed to obtain a 3D reconstruction from non-crystalline specimens (“single particles”) using the so-called Random Conical Tilt Series Method. We start from a pair of images presenting the same field, first tilted (by ≃55°) and then untilted. It is then assumed that we can supply the system with the image of the particle we are looking for (ideally, a 2D average from a previous study) and with a matrix describing the geometrical relationships between the tilted and untilted fields (this step is now accomplished by interactively marking a few pairs of corresponding features in the two fields). From here on the 3D reconstruction process may be run automatically.


Author(s):  
Adriana Verschoor ◽  
Ronald Milligan ◽  
Suman Srivastava ◽  
Joachim Frank

We have studied the eukaryotic ribosome from two vertebrate species (rabbit reticulocyte and chick embryo ribosomes) in several different electron microscopic preparations (Fig. 1a-d), and we have applied image processing methods to two of the types of images. Reticulocyte ribosomes were examined in both negative stain (0.5% uranyl acetate, in a double-carbon preparation) and frozen hydrated preparation as single-particle specimens. In addition, chick embryo ribosomes in tetrameric and crystalline assemblies in frozen hydrated preparation have been examined. 2D averaging, multivariate statistical analysis, and classification methods have been applied to the negatively stained single-particle micrographs and the frozen hydrated tetramer micrographs to obtain statistically well defined projection images of the ribosome (Fig. 2a,c). 3D reconstruction methods, the random conical reconstruction scheme and weighted back projection, were applied to the negative-stain data, and several closely related reconstructions were obtained. The principal 3D reconstruction (Fig. 2b), which has a resolution of 3.7 nm according to the differential phase residual criterion, can be compared to the images of individual ribosomes in a 2D tetramer average (Fig. 2c) at a similar resolution, and a good agreement of the general morphology and of many of the characteristic features is seen.Both data sets show the ribosome in roughly the same ’view’ or orientation, with respect to the adsorptive surface in the electron microscopic preparation, as judged by the agreement in both the projected form and the distribution of characteristic density features. The negative-stain reconstruction reveals details of the ribosome morphology; the 2D frozen-hydrated average provides projection information on the native mass-density distribution within the structure. The 40S subunit appears to have an elongate core of higher density, while the 60S subunit shows a more complex pattern of dense features, comprising a rather globular core, locally extending close to the particle surface.


Author(s):  
Alexander S. Lelekov ◽  
Rudolf P. Trenkenshu

The paper presents an example of the linear splines use to describe the photosynthesis light curves for microalgae culture. The main mathematical models of the relationship between photosynthesis rate and light are listed. Based on the previously formulated basic principles of modeling microalgae photobiosynthesis, a mathematical model is proposed that describes the dependence of the assimilation number of chlorophyll a on the value of the light flux by linear splines. The advantage of the proposed approach is a clear definition of the point of change of the limiting factor. It is shown that light-limited photosynthesis rate is determined not only by external irradiation, but also by the concentration of chlorophyll a. The light-saturated rate depends on the amount of a key enzyme complex, which limits the rate of energy exchange reactions in the cell. Verification of the proposed model on the example of the diatom microalgae Skeletonema costatum was carried out. It is shown that the higher the degree of cell adaptation to high irradiation, the better the photosynthesis curve is described by linear splines. If S. costatum cells are adapted to low irradiation, deviations of experimental data from the idealized broken line are observed, which are caused by changes in the pigment composition. When the experimental data are normalized, the cell adaptation factor is reduced, all points are described by a single broken line, which indicates the universality of the proposed approach.


2013 ◽  
Vol 3 (2) ◽  
pp. 111-115 ◽  
Author(s):  
Wendy Babcox

Every Olive Tree in the Garden of Gethsemane is a suite of photographic images of each of the twenty-three olive trees in the garden. Situated at the foot of the Mount of Olives in Jerusalem, the Garden of Gethsemane is known to many as the site where Jesus and his disciples prayed the night before his crucifixion. The oldest trees in the garden date to 1092 and are recognized as some of the oldest olive trees in existence. The older trees are a living and symbolic connection to the distant past, while younger trees serve as a link to the future. The gnarled trunks seem written with the many conflicts that have been waged in an effort to control this most-contested city; a city constantly on the threshold of radical transformation.


1981 ◽  
Vol 101 (11) ◽  
pp. 698-698
Author(s):  
Itsuya Muta ◽  
Eiichi Mukai ◽  
Masaru Yagi

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