scholarly journals Multiplexed force measurements on live cells with holographic optical tweezers

2009 ◽  
Vol 17 (8) ◽  
pp. 6209 ◽  
Author(s):  
Cecile O. Mejean ◽  
Andrew W. Schaefer ◽  
Eleanor A. Millman ◽  
Paul Forscher ◽  
Eric R. Dufresne
Keyword(s):  
Optical Tweezers ◽  
Live Cells ◽  
Force Measurements ◽  
Holographic Optical Tweezers

Tomographic phase microscopy with 180° rotation of live cells in suspension by holographic optical tweezers

2015 ◽  
Vol 40 (8) ◽  
pp. 1881 ◽  
Author(s):  
Mor Habaza ◽  
Barak Gilboa ◽  
Yael Roichman ◽  
Natan T. Shaked
Keyword(s):  
Optical Tweezers ◽  
Live Cells ◽  
Phase Microscopy ◽  
Holographic Optical Tweezers

Moving average process underlying the holographic–optical–tweezers experiments

2014 ◽  
Vol 53 (10) ◽  
pp. B254 ◽  
Author(s):  
Jakub Ślęzak ◽  
Sławomir Drobczyński ◽  
Karina Weron ◽  
Jan Masajada
Keyword(s):  
Optical Tweezers ◽  
Moving Average ◽  
Average Process ◽  
Moving Average Process ◽  
Holographic Optical Tweezers

scholarly journals Hemodynamic forces can be accurately measured in vivo with optical tweezers

2017 ◽  
Author(s):  
Sébastien Harlepp ◽  
Fabrice Thalmann ◽  
Gautier Follain ◽  
Jacky G. Goetz
Keyword(s):  
Optical Tweezers ◽  
Cell Biology ◽  
Accurate Determination ◽  
Force Measurements ◽  
Hemodynamic Forces ◽  
Non Invasive ◽  
Drag Forces ◽  
Measured Flow ◽  
Living Organisms

AbstractForce sensing and generation at the tissular and cellular scale is central to many biological events. There is a growing interest in modern cell biology for methods enabling force measurements in vivo. Optical trapping allows non-invasive probing of pico-Newton forces and thus emerged as a promising mean for assessing biomechanics in vivo. Nevertheless, the main obstacles rely in the accurate determination of the trap stiffness in heterogeneous living organisms, at any position where the trap is used. A proper calibration of the trap stiffness is thus required for performing accurate and reliable force measurements in vivo. Here, we introduce a method that overcomes these difficulties by accurately measuring hemodynamic profiles in order to calibrate the trap stiffness. Doing so, and using numerical methods to assess the accuracy of the experimental data, we measured flow profiles and drag forces imposed to trapped red blood cells of living zebrafish embryos. Using treatments enabling blood flow tuning, we demonstrated that such method is powerful in measuring hemodynamic forces in vivo with accuracy and confidence. Altogether, this study demonstrates the power of optical tweezing in measuring low range hemodynamic forces in vivo and offers an unprecedented tool in both cell and developmental biology.

scholarly journals Precise, contactless measurements of the surface tension of picolitre aerosol droplets

2016 ◽  
Vol 7 (1) ◽  
pp. 274-285 ◽  
Author(s):  
Bryan R. Bzdek ◽  
Rory M. Power ◽  
Stephen H. Simpson ◽  
Jonathan P. Reid ◽  
C. Patrick Royall
Keyword(s):  
Surface Tension ◽  
Optical Tweezers ◽  
Holographic Optical Tweezers ◽  
Contactless Measurements

Precise measurements of the surface tension and viscosity of airborne picolitre droplets can be accomplished using holographic optical tweezers.

Aberration correction in holographic optical tweezers using a high-order optical vortex: publisher’s note

2018 ◽  
Vol 57 (17) ◽  
pp. 4857
Author(s):  
Yansheng Liang ◽  
Yanan Cai ◽  
Zhaojun Wang ◽  
Ming Lei ◽  
Zhiliang Cao ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Optical Vortex ◽  
High Order ◽  
Aberration Correction ◽  
Holographic Optical Tweezers

Wavefront engineering and holographic optical tweezers

2015 ◽  
pp. 319-344
Author(s):  
Philip Jones ◽  
Onofrio Marago ◽  
Giovanni Volpe
Keyword(s):  
Optical Tweezers ◽  
Holographic Optical Tweezers

scholarly journals Assembly of 3-dimensional structures using programmable holographic optical tweezers

2004 ◽  
Vol 12 (22) ◽  
pp. 5475 ◽  
Author(s):  
Gavin Sinclair ◽  
Pamela Jordan ◽  
Johannes Courtial ◽  
Miles Padgett ◽  
Jon Cooper ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
3 Dimensional ◽  
Holographic Optical Tweezers

Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

2001 ◽  
Author(s):  
Bahman Anvari ◽  
Zhiwei Li ◽  
Masayoshi Takashima ◽  
Peter Brecht ◽  
Jorge H. Torres ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Optical Tweezers ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
Force Measurements
Sign in / Sign up

Export Citation Format

Share Document