scholarly journals Acoustical nanometre-scale vibrations of live cells detected by a near-field optical setup

2007 ◽  
Vol 15 (9) ◽  
pp. 5589 ◽  
Author(s):  
Rosaria Piga ◽  
Ruggero Micheletto ◽  
Yoichi Kawakami
Keyword(s):  
Near Field ◽  
Live Cells ◽  
Optical Setup

Dichroism of diamond grains by a polarization modulated near field optical setup

2006 ◽  
Vol 89 (12) ◽  
pp. 121125 ◽  
Author(s):  
Ruggero Micheletto ◽  
Yoichi Kawakami ◽  
Claudio Manfredotti ◽  
Yiuri Garino ◽  
Maria Allegrini
Keyword(s):  
Near Field ◽  
Optical Setup

Multi Layer Disk Design For Near Field Phase-Change Recording

2003 ◽  
Vol 803 ◽  
Author(s):  
Koichiro Kishima ◽  
Isao Ichimura ◽  
Kimihiro Saito ◽  
Kenji Yamamoto ◽  
Atsushi Iida ◽  
...  
Keyword(s):  
Refractive Index ◽  
Liquid Crystal ◽  
Layer Structure ◽  
Near Field ◽  
Numerical Aperture ◽  
High Refractive Index ◽  
Field Phase ◽  
Optical Setup ◽  
Field Recording ◽  
Signal Characteristics

ABSTRACTWe propose multi-layer near-field recording with using inner focus mode. The restricted conditions for multi-layer near-field recording are discussed from the viewpoint of both media structure and optical setup. One solution is presented for dual layer recording with a numerical aperture (NA) of 1.5 and a wavelength of 405 nm as a light source of GaN laser diode.In the proposed layer structure, a Nb2O5 material has been adopted as the intermediate layer because of its high refractive index, n = 2.4, in order to prevent the decrease of beam propagation that corresponds to NA>1. Almost the same signal characteristics can be obtained from both recording layers at the air gap of 40 nm. The adjustment of focusing position and the compensation of spherical aberrations have been implemented by the combination of an expander lens unit and a liquid crystal (LC) panel in the optical setup.

Biological Imaging with a Near-Field Optical Setup

2003 ◽  
Vol 3 (6) ◽  
pp. 496-502 ◽  
Author(s):  
Morgan Denyer ◽  
Ruggero Micheletto ◽  
Ken Nakajima ◽  
Masahiko Hara ◽  
Satoshi Okazaki
Keyword(s):  
Near Field ◽  
Biological Imaging ◽  
Optical Setup

scholarly journals Clog-Free Trilobite Filtration: Tunable Flow Setup and Velocity Measurements

Micromachines ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 904
Author(s):  
Endre Joachim Mossige ◽  
Atle Jensen
Keyword(s):  
Flow Fields ◽  
Live Cells ◽  
Device Performance ◽  
Flow Profile ◽  
Rigid Spheres ◽  
Persistent Problem ◽  
Flow Configuration ◽  
Optical Setup ◽  
Micro Particle Image Velocimetry ◽  
Filter Structures

The ability to separate and filter out microscopic objects lies at the core of many biomedical applications. However, a persistent problem is clogging, as biomaterials stick to the internal chip surface and limit device efficiency and liability. Here, we review an alternative technique that could solve these clogging issues. By leveraging tunable flow fields and particle inertia around special trilobite-shaped filtration units, we perform filtration of plastic beads by size and we demonstrate sorting of live cells. The separation and filtration are performed completely without signs of clogging. However, a clog-free operation relies on a controlled flow configuration to steer the particles and cells away from the filter structures. In this paper, we describe the tunable flow system for such an operation and we describe an optical setup enabling hydrodynamical interactions between particles and cells with the flow fields and direct interactions with the filter structures to be characterized. The optical setup is capable of measuring particle and flow velocities (by Particle Tracking Velocimetry (PTV), Micro Particle Image Velocimetry (μPIV), and streakline visualization) in meters per second necessary to avoid clogging. However, accurate measurements rely on strict calibration and validation procedures to be followed, and we devote a substantial portion of our paper to laying out such procedures. A comparison between μPIV data and a known flow profile is particularly valuable for assessing measurement accuracy, and this important validation has not been previously published by us. The detail level in our description of the flow configuration and optical system is sufficient to replicate the experiments. In the last part of the paper, we review an assessment of the device performance when handling rigid spheres and live cells. We deconvolute the influences of cell shape from effects of size and find that the shape has only a weak influence on device performance.

scholarly journals Scanning near-field optical microscopy of live cells in liquid

2005 ◽  
Vol 45 (3) ◽  
pp. 207-211
Author(s):  
Regimantas Januškevičius ◽  
Viktoras Vaičikauskas ◽  
D. J. Arndt-Jovin ◽  
T. M. Jovin
Keyword(s):  
Optical Microscopy ◽  
Near Field ◽  
Live Cells

Study of the fluorescence of perylene cation radical salts with a near field optical setup

2002 ◽  
Vol 280 (12) ◽  
pp. 1067-1074 ◽  
Author(s):  
Ruggero Micheletto ◽  
Jun Matsui ◽  
Nobuki Yoshimatsu ◽  
Munetaka Oyama ◽  
Satoshi Okazaki
Keyword(s):  
Near Field ◽  
Cation Radical ◽  
Optical Setup

scholarly journals Cell biology beyond the diffraction limit: near-field scanning optical microscopy

2001 ◽  
Vol 114 (23) ◽  
pp. 4153-4160
Author(s):  
Frank de Lange ◽  
Alessandra Cambi ◽  
Richard Huijbens ◽  
Bärbel de Bakker ◽  
Wouter Rensen ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Single Molecule ◽  
Cell Biology ◽  
Imaging Techniques ◽  
Near Field ◽  
Diffraction Limit ◽  
Detection Sensitivity ◽  
Live Cells ◽  
Scanning Optical Microscopy ◽  
Near Field Scanning

Throughout the years, fluorescence microscopy has proven to be an extremely versatile tool for cell biologists to study live cells. Its high sensitivity and non-invasiveness, together with the ever-growing spectrum of sophisticated fluorescent indicators, ensure that it will continue to have a prominent role in the future. A drawback of light microscopy is the fundamental limit of the attainable spatial resolution – ∼250 nm – dictated by the laws of diffraction. The challenge to break this diffraction limit has led to the development of several novel imaging techniques. One of them, near-field scanning optical microscopy (NSOM), allows fluorescence imaging at a resolution of only a few tens of nanometers and, because of the extremely small near-field excitation volume, reduces background fluorescence from the cytoplasm to the extent that single-molecule detection sensitivity becomes within reach. NSOM allows detection of individual fluorescent proteins as part of multimolecular complexes on the surface of fixed cells, and similar results should be achievable under physiological conditions in the near future.

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Near-field scanning optical microscopy

1986 ◽  
Vol 44 ◽  
pp. 642-643
Author(s):  
E. Betzig ◽  
A. Harootunian ◽  
M. Isaacson ◽  
A. Lewis
Keyword(s):  
Near Field ◽  
Optical Microscope ◽  
Visible Radiation ◽  
Field Methods ◽  
Optical Elements ◽  
Optical Region ◽  
Order Of Magnitude ◽  
Nondestructive Imaging ◽  
Scanning Optical Microscopy ◽  
Near Field Scanning

In general, conventional methods of optical imaging are limited in spatial resolution by either the wavelength of the radiation used or by the aberrations of the optical elements. This is true whether one uses a scanning probe or a fixed beam method. The reason for the wavelength limit of resolution is due to the far field methods of producing or detecting the radiation. If one resorts to restricting our probes to the near field optical region, then the possibility exists of obtaining spatial resolutions more than an order of magnitude smaller than the optical wavelength of the radiation used. In this paper, we will describe the principles underlying such "near field" imaging and present some preliminary results from a near field scanning optical microscope (NS0M) that uses visible radiation and is capable of resolutions comparable to an SEM. The advantage of such a technique is the possibility of completely nondestructive imaging in air at spatial resolutions of about 50nm.

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