scholarly journals Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting

2003 ◽  
Vol 11 (26) ◽  
pp. 3583 ◽  
Author(s):  
Michael Wahl ◽  
Ingo Gregor ◽  
Mattias Patting ◽  
Jörg Enderlein
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Fast Calculation ◽  
Single Photon Counting ◽  
Fluorescence Correlation ◽  
Correlation Data

scholarly journals Single-molecule detection on a portable 3D-printed microscope

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
James W. P. Brown ◽  
Arnaud Bauer ◽  
Mark E Polinkovsky ◽  
Akshay Bhumkar ◽  
Dominic J. B. Hunter ◽  
...  
Keyword(s):  
Single Molecule ◽  
Amyloid Fibrils ◽  
Single Photon ◽  
Photon Counting ◽  
Correlation Spectroscopy ◽  
Single Molecule Detection ◽  
Single Photon Counting ◽  
Fluorescence Correlation ◽  
3D Printed ◽  
Simple Format

AbstractSingle-molecule assays have, by definition, the ultimate sensitivity and represent the next frontier in biological analysis and diagnostics. However, many of these powerful technologies require dedicated laboratories and trained personnel and have therefore remained research tools for specialists. Here, we present a single-molecule confocal system built from a 3D-printed scaffold, resulting in a compact, plug and play device called the AttoBright. This device performs single photon counting and fluorescence correlation spectroscopy (FCS) in a simple format and is widely applicable to the detection of single fluorophores, proteins, liposomes or bacteria. The power of single-molecule detection is demonstrated by detecting single α-synuclein amyloid fibrils, that are currently evaluated as biomarkers for Parkinson’s disease, with an improved sensitivity of >100,000-fold over bulk measurements.

scholarly journals FoCuS-point: software for STED fluorescence correlation and time-gated single photon counting

2015 ◽  
Vol 32 (6) ◽  
pp. 958-960 ◽  
Author(s):  
Dominic Waithe ◽  
Mathias P. Clausen ◽  
Erdinc Sezgin ◽  
Christian Eggeling
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Super Resolution ◽  
Correlation Spectroscopy ◽  
Single Photon Counting ◽  
Fluorescence Correlation ◽  
Point Data ◽  
User Friendly ◽  
Kinetics Of ◽  
Focus Point

Abstract Motivation: Fluorescence Correlation Spectroscopy (FCS) is a popular tool for measuring molecular mobility and how mobility relates to molecular interaction dynamics and bioactivity in living cells. The FCS technique has been significantly advanced by its combination with super-resolution STED microscopy (STED-FCS). Specifically, the use of gated detection has shown great potential for enhancing STED-FCS, but has also created a demand for software which is efficient and also implements the latest algorithms. Prior to this study, no open software has been available which would allow practical time-gating and correlation of point data derived from STED-FCS experiments. Results: The product of this study is a piece of stand-alone software called FoCuS-point. FoCuS-point utilizes advanced time-correlated single-photon counting (TCSPC) correlation algorithms along with time-gated filtering and innovative data visualization. The software has been designed to be highly user-friendly and is tailored to handle batches of data with tools designed to process files in bulk. FoCuS-point also includes advanced fitting algorithms which allow the parameters of the correlation curves and thus the kinetics of diffusion to be established quickly and efficiently. Availability and implementation: FoCuS-point is written in python and is available through the github repository: https://github.com/dwaithe/FCS_point_correlator. Furthermore, compiled versions of the code are available as executables which can be run directly in Linux, Windows and Mac OSX operating systems. Contact: [email protected]

Single Point PICA Probing with an Avalanche Photo-Diode

2001 ◽  
Author(s):  
Mike Bruce ◽  
Rama R. Goruganthu ◽  
Shawn McBride ◽  
David Bethke ◽  
J.M. Chin
Keyword(s):  
Single Photon ◽  
Single Point ◽  
Photon Counting ◽  
Ring Oscillator ◽  
Time Resolved ◽  
Single Photon Counting ◽  
Photo Diode ◽  
Avalanche Photo Diode ◽  
Photo Multiplier Tube ◽  
Channel Plate

Abstract For time resolved hot carrier emission from the backside, an alternate approach is demonstrated termed single point PICA. The single point approach records time resolved emission from an individual transistor using time-correlated-single-photon counting and an avalanche photo-diode. The avalanche photo-diode has a much higher quantum efficiency than micro-channel plate photo-multiplier tube based imaging cameras typically used in earlier approaches. The basic system is described and demonstrated from the backside on a ring oscillator circuit.

scholarly journals UVscope and its application aboard the ASTRI-Horn telescope

2021 ◽  
Author(s):  
Maria Concetta Maccarone ◽  
Giovanni La Rosa ◽  
Osvaldo Catalano ◽  
Salvo Giarrusso ◽  
Alberto Segreto ◽  
...  
Keyword(s):  
Gamma Ray ◽  
Single Photon ◽  
Photon Counting ◽  
Light Flux ◽  
Cosmic Ray ◽  
High Energy ◽  
Wide Field ◽  
High Energy Astrophysics ◽  
Single Photon Counting ◽  
Photon Counting Mode

AbstractUVscope is an instrument, based on a multi-pixel photon detector, developed to support experimental activities for high-energy astrophysics and cosmic ray research. The instrument, working in single photon counting mode, is designed to directly measure light flux in the wavelengths range 300-650 nm. The instrument can be used in a wide field of applications where the knowledge of the nocturnal environmental luminosity is required. Currently, one UVscope instrument is allocated onto the external structure of the ASTRI-Horn Cherenkov telescope devoted to the gamma-ray astronomy at very high energies. Being co-aligned with the ASTRI-Horn camera axis, UVscope can measure the diffuse emission of the night sky background simultaneously with the ASTRI-Horn camera, without any interference with the main telescope data taking procedures. UVscope is properly calibrated and it is used as an independent reference instrument for test and diagnostic of the novel ASTRI-Horn telescope.

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