Single-molecule nanosecond rotational diffusion analysis by time-correlated single-photon counting system

2013 ◽  
Author(s):  
Dror Fixler ◽  
Lior Turgeman
Keyword(s):  
Single Molecule ◽  
Single Photon ◽  
Rotational Diffusion ◽  
Photon Counting ◽  
Counting System ◽  
Single Photon Counting ◽  
Diffusion Analysis

Compact, Low-Power and Fully Reconfigurable 10 ps Resolution, 160 Range, Time-Resolved Single-Photon Counting System

2016 ◽  
Vol 16 (10) ◽  
pp. 3827-3833 ◽  
Author(s):  
Davide Tamborini ◽  
Mauro Buttafava ◽  
Alessandro Ruggeri ◽  
Franco Zappa
Keyword(s):  
Low Power ◽  
Single Photon ◽  
Photon Counting ◽  
Counting System ◽  
Time Resolved ◽  
Single Photon Counting

Single‐photon counting system for measuring laser parameters

1979 ◽  
Vol 50 (8) ◽  
pp. 1013-1017
Author(s):  
T. G. Miller ◽  
D. R. Womack ◽  
J. R. Williams ◽  
M. J. Monahan ◽  
Q. C. Murphree
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Counting System ◽  
Single Photon Counting ◽  
Laser Parameters

scholarly journals Development of new photon-counting detectors for single-molecule fluorescence microscopy

2013 ◽  
Vol 368 (1611) ◽  
pp. 20120035 ◽  
Author(s):  
X. Michalet ◽  
R. A. Colyer ◽  
G. Scalia ◽  
A. Ingargiola ◽  
R. Lin ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Single Photon ◽  
Photon Counting ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Large Area ◽  
Single Molecule Fluorescence ◽  
Single Photon Counting ◽  
Single Molecule Fluorescence Microscopy

Two optical configurations are commonly used in single-molecule fluorescence microscopy: point-like excitation and detection to study freely diffusing molecules, and wide field illumination and detection to study surface immobilized or slowly diffusing molecules. Both approaches have common features, but also differ in significant aspects. In particular, they use different detectors, which share some requirements but also have major technical differences. Currently, two types of detectors best fulfil the needs of each approach: single-photon-counting avalanche diodes (SPADs) for point-like detection, and electron-multiplying charge-coupled devices (EMCCDs) for wide field detection. However, there is room for improvements in both cases. The first configuration suffers from low throughput owing to the analysis of data from a single location. The second, on the other hand, is limited to relatively low frame rates and loses the benefit of single-photon-counting approaches. During the past few years, new developments in point-like and wide field detectors have started addressing some of these issues. Here, we describe our recent progresses towards increasing the throughput of single-molecule fluorescence spectroscopy in solution using parallel arrays of SPADs. We also discuss our development of large area photon-counting cameras achieving subnanosecond resolution for fluorescence lifetime imaging applications at the single-molecule level.

Use of a synchronously pumped picosecond laser for excitation of a spectrofluorimetric single-photon counting system

1983 ◽  
Vol 13 (8) ◽  
pp. 1132-1134
Author(s):  
S F Ivanov ◽  
A V Ostrovskiĭ ◽  
P G Pleshanov ◽  
K A Turkin ◽  
V S Fokin ◽  
...  
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Picosecond Laser ◽  
Counting System ◽  
Single Photon Counting ◽  
Synchronously Pumped

A pixel detector-based single photon-counting system as fast spectrometer for diagnostic x-ray beams

2008 ◽  
Vol 129 (1-3) ◽  
pp. 119-122 ◽  
Author(s):  
C. Carpentieri ◽  
M. G. Bisogni ◽  
A. Del Guerra ◽  
P. Delogu ◽  
M. E. Fantacci ◽  
...  
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Pixel Detector ◽  
Counting System ◽  
Single Photon Counting ◽  
X Ray

Picosecond Tryptophan Fluorescence of Human Blood Serum Orosomucoid

1996 ◽  
Vol 61 (5) ◽  
pp. 808-818 ◽  
Author(s):  
Martin Hof ◽  
Stefan Vajda ◽  
Vlastimil Fidler ◽  
Vladimír Karpenko
Keyword(s):  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Tryptophan Fluorescence ◽  
Fluorescence Decay ◽  
Amino Acid Residues ◽  
Counting System ◽  
Single Photon Counting ◽  
Decay Behaviour ◽  
The Individual

The state of three tryptophyl residues in human serum orosomucoid was estimated by prediction methods based on parameters characterizing their hydrophobicity either directly, or in terms of buried surfaces of the individual amino acid residues. It is shown that tryptophan 25 is the most buried, while Trp 160 is the most exposed to the solvent. Trp 122 is in this respect in an intermediate state. The fluorescence decay behaviour was determined using a picosecond single photon counting system. The multiwavelength data were analyzed using a global analysis as well as a distribution of lifetimes program. Both procedures yielded the existence of four wavelength independent lifetimes (0.22 ns, 1.0 ns, 2.5 ns, and 8.4 ns). A tentative assignment of the decay associated spectra of the four components to the three individual tryptophans is presented.

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