Fabry-Perot Fourier-Transform Hyperspectral Imaging for High Efficiency Fluorescence Microscopy

2012 ◽  
Author(s):  
Marco Pisani ◽  
Massimo Zucco ◽  
Paolo Bianco
Keyword(s):  
Fourier Transform ◽  
Fluorescence Microscopy ◽  
Hyperspectral Imaging ◽  
High Efficiency ◽  
Fabry Perot

scholarly journals On Performance of Sparse Fast Fourier Transform Algorithms Using the Aliasing Filter

Electronics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1117
Author(s):  
Bin Li ◽  
Zhikang Jiang ◽  
Jie Chen
Keyword(s):  
Fourier Transform ◽  
Fast Fourier Transform ◽  
High Efficiency ◽  
Theory And Practice ◽  
Long Time ◽  
And Performance ◽  
Binary Tree Search ◽  
The One ◽  
Sparse Fast Fourier Transform ◽  
L1 And L2

Computing the sparse fast Fourier transform (sFFT) has emerged as a critical topic for a long time because of its high efficiency and wide practicability. More than twenty different sFFT algorithms compute discrete Fourier transform (DFT) by their unique methods so far. In order to use them properly, the urgent topic of great concern is how to analyze and evaluate the performance of these algorithms in theory and practice. This paper mainly discusses the technology and performance of sFFT algorithms using the aliasing filter. In the first part, the paper introduces the three frameworks: the one-shot framework based on the compressed sensing (CS) solver, the peeling framework based on the bipartite graph and the iterative framework based on the binary tree search. Then, we obtain the conclusion of the performance of six corresponding algorithms: the sFFT-DT1.0, sFFT-DT2.0, sFFT-DT3.0, FFAST, R-FFAST, and DSFFT algorithms in theory. In the second part, we make two categories of experiments for computing the signals of different SNRs, different lengths, and different sparsities by a standard testing platform and record the run time, the percentage of the signal sampled, and the L0, L1, and L2 errors both in the exactly sparse case and the general sparse case. The results of these performance analyses are our guide to optimize these algorithms and use them selectively.

scholarly journals Amplitude electro-optical modulation of radiation by sequences of Fabry-Perot resonators

2020 ◽  
Vol 56 (2) ◽  
pp. 217-223
Author(s):  
V. B. Zalesski ◽  
A. I. Konoiko ◽  
V. M. Kravchenko ◽  
H. S. Kuzmitskaya
Keyword(s):  
Lithium Niobate ◽  
High Efficiency ◽  
Optical Modulation ◽  
Operating Point ◽  
Optical Modulator ◽  
Control Voltage ◽  
Clock Frequency ◽  
Fabry Perot ◽  
In Series ◽  
Light Signals

In this paper, we considered the method of amplitude electro-optical modulation of radiation using sequences of Fabry-Perot resonators based on the transverse electro-optical effect on the example of lithium niobate LiNbO3. With this method, it is possible to significantly reduce the voltage of the control electromagnetic field of the electro-optical amplitude modulator operating in the transmission mode of the light beam while maintaining its high efficiency. The reduction of the control voltage is achieved by increasing the number of Fabry-Perot resonators installed in series and the phase shift relative to the extremum of the transmittance function. This method allows to diminish the duration of the received light signals which leads to an increase in the clock frequency while maintaining a high efficiency of the radiation modulation. Diminishing the duration of light signals is achieved by using separate modulation channels of two sequences of electro-optical Fabry-Perot resonators, the first of which works on the transmission and the second one on the reflection. Increasing the clock frequency at the output of the modulator is achieved by summing the signals coming from several modulation channels. It is shown that the value of the control voltage for an amplitude electro-optical modulator based on a sequence of Fabry-Perot resonators made of lithium niobate LiNbO3, with an operating wavelength of 1.307 microns, can be 4 V in the case when its initial operating point corresponds to the maximum transmittance. The control voltage is 2 V if the initial operating point is shifted in phase relative to the extremum of the transmittance function.

An Efficient Galerkin Averaging-Incremental Harmonic Balance Method Based on the Fast Fourier Transform and Tensor Contraction

2020 ◽  
Author(s):  
R. Ju ◽  
W. Fan ◽  
W. D. Zhu
Keyword(s):  
Fourier Transform ◽  
Fast Fourier Transform ◽  
Harmonic Balance ◽  
High Efficiency ◽  
Jacobian Matrix ◽  
Sampling Rate ◽  
Harmonic Balance Method ◽  
Incremental Harmonic Balance ◽  
Tensor Contraction ◽  
Ihb Method

Abstract An efficient Galerkin averaging-incremental harmonic balance (EGA-IHB) method is developed based on the fast Fourier transform (FFT) and tensor contraction to increase efficiency and robustness of the IHB method when calculating periodic responses of complex nonlinear systems with non-polynomial nonlinearities. As a semi-analytical method, derivation of formulae and programming are significantly simplified in the EGA-IHB method. The residual vector and Jacobian matrix corresponding to nonlinear terms in the EGA-IHB method are expressed using truncated Fourier series. After calculating Fourier coefficient vectors using the FFT, tensor contraction is used to calculate the Jacobian matrix, which can significantly improve numerical efficiency. Since inaccurate results may be obtained from discrete Fourier transform-based methods when aliasing occurs, the minimal non-aliasing sampling rate is determined for the EGA-IHB method. Performances of the EGA-IHB method are analyzed using several benchmark examples; its accuracy, efficiency, convergence, and robustness are analyzed and compared with several widely used semi-analytical methods. The EGA-IHB method has high efficiency and good robustness for both polynomial and nonpolynomial nonlinearities, and it has considerable advantages over the other methods.

Stimulated release of fluorescently labeled IgE fragments that efficiently accumulate in secretory granules after endocytosis in RBL-2H3 mast cells

1998 ◽  
Vol 111 (16) ◽  
pp. 2385-2396 ◽  
Author(s):  
K. Xu ◽  
R.M. Williams ◽  
D. Holowka ◽  
B. Baird
Keyword(s):  
Mast Cells ◽  
Fluorescence Microscopy ◽  
High Efficiency ◽  
Immunoglobulin E ◽  
Secretory Granules ◽  
Dose Dependence ◽  
Secretory Vesicles ◽  
Release Process ◽  
Intracellular Compartments ◽  
Stimulation Of

Sensitization of RBL-2H3 mast cells with monomeric fluorescein-5-isothiocyanate (FITC)-labeled immunoglobulin E (IgE) results in slow but highly efficient accumulation of labeled IgE fragments in a pool of acidic peripheral vesicles that are visible by fluorescence microscopy after raising endosomal pH with ammonium chloride. Stimulation of cells containing these FITC-IgE fragments by aggregation of high affinity receptors for IgE (FcepsilonRI) or by Ca2+ ionophore and phorbol 12-myristate 13-acetate results in release of FITC fluorescence from the cells, which can be monitored continuously with a spectrofluorometer. The fluorescence release process corresponds to cellular degranulation: it is prevented under conditions that prevent stimulated beta-hexosaminidase release, and these two processes exhibit the same antigen dose-dependence and kinetics. Pulse-chase labeling reveals that aggregation of FITC-IgE bound to FcepsilonRI at the cell surface causes internalization and delivery to the regulated secretory vesicles with a high efficiency similar to monomeric IgE-FcepsilonRI, but more rapidly. Binding of Cy3-modified IgE to FcepsilonRI results in labeling of the same secretory vesicles as in FITC-IgE-sensitized cells, and these Cy3-labeled vesicles can be observed by fluorescence microscopy without neutralization of intracellular compartments. Simultaneous three-photon microscopy of serotonin fluorescence and two-photon microscopy of Cy3 fluorescence reveals that these Cy3-labeled vesicles coincide with serotonin-labeled secretory granules. After stimulation of the cells via aggregation of IgE-FcepsilonRI or addition of Ca2+ ionophore and phorbol 12-myristate 13-acetate, depletion of the Cy3 label from the intracellular vesicles is observed with confocal microscopy. These results provide strong evidence for the lysosomal nature of secretory granules in these cells. In addition, they provide the basis for a direct, real-time method for monitoring single cell degranulation.

scholarly journals New Experimental Possibilities and Future Prospects for 1-5μm Infrared Spectroscopy of Interstellar Molecules

1980 ◽  
Vol 87 ◽  
pp. 631-632
Author(s):  
Donald N. B. Hall
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Millimeter Wave ◽  
Molecular Clouds ◽  
Future Prospects ◽  
Interstellar Molecules ◽  
The Past ◽  
Evolved Stars ◽  
Large Numbers ◽  
Fabry Perot

During the past week we have heard a considerable number of papers dealing with spectroscopic observations in the 1-5μm region of the infrared. I predict that as instruments and detectors continue to improve, such observations will play a major role in the study of interstellar molecules in both molecular clouds and circumstellar clouds around evolved stars. Fourier Transform spectrometers and Fabry Perot interferometers have already yielded spectra of such sources with spectral resolution and radial velocity precision fully comparable to millimeter wave observations. The critical need is to improve the limiting magnitude of such observations by 3-5 stellar magnitudes so that one can study large numbers of sources rather than the few brightest in each class.

scholarly journals In-line FINCH super resolution digital holographic fluorescence microscopy using a high efficiency transmission liquid crystal GRIN lens

2013 ◽  
Vol 38 (24) ◽  
pp. 5264 ◽  
Author(s):  
Gary Brooker ◽  
Nisan Siegel ◽  
Joseph Rosen ◽  
Nobuyuki Hashimoto ◽  
Makoto Kurihara ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Fluorescence Microscopy ◽  
High Efficiency ◽  
Super Resolution ◽  
Grin Lens

Vibroacoustic Comparisons and Acoustic Power Insulation Mechanisms of Multidirectional Stiffened Laminated Plates

2015 ◽  
Vol 137 (2) ◽  
Author(s):  
Xiongtao Cao ◽  
Hongxing Hua
Keyword(s):  
Fourier Transform ◽  
High Efficiency ◽  
Three Dimensional ◽  
Acoustic Power ◽  
Higher Order ◽  
Laminated Plates ◽  
Transverse Displacement ◽  
Third Order ◽  
First Order ◽  
Implicit Equations

Vibroacoustic characteristics of multidirectional stiffened laminated plates with or without compliant layers are explored in the wavenumber and spatial domains with the help of the two-dimensional continuous Fourier transform and discrete inverse fast Fourier transform. Implicit equations of motion for the arbitrary angle ply laminated plates are derived from the three-dimensional higher order and Reddy third order shear deformation plate theories. The expressions of acoustic power of the stiffened laminated plates with or without complaint layers are formulated in the wavenumber domain, which is a significant method to calculate acoustic power of the stiffened plates with multiple sets of cross stiffeners. Vibroacoustic comparisons of the stiffened laminated plates are made in terms of the transverse displacement spectra, forced responses, acoustic power, and input power according to the first order, Reddy third order, and three-dimensional higher order plate theories. Sound reduction profiles of compliant layers are further examined by the theoretical deductions. This study shows the feasibility and high efficiency of the first order and Reddy third order plate theories in the broad frequency range and allows a better understanding the principal mechanisms of acoustic power radiated from multidirectional stiffened laminated composite plates with compliant layers, which has not been adequately addressed in its companion paper. (Cao and Hua, 2012, “Sound Radiation From Shear Deformable Stiffened Laminated Plates With Multiple Compliant Layers,” ASME J. Vib. Acoust., 134(5), p. 051001.)

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