Optimal Point Spread Function Engineering for 3D Super-Resolution Imaging

2014 ◽  
Author(s):  
Yoav Shechtman ◽  
Steffen J. Sahl ◽  
Adam S. Backer ◽  
W. E. Moerner
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Optimal Point ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering ◽  
Resolution Imaging

scholarly journals High-fidelity structured illumination microscopy by point-spread-function engineering

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Gang Wen ◽  
Simin Li ◽  
Linbo Wang ◽  
Xiaohu Chen ◽  
Zhenglong Sun ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
High Fidelity ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Imaging Tool ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering

AbstractStructured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.

The changing point-spread function: single-molecule-based super-resolution imaging

2014 ◽  
Vol 141 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Mathew H. Horrocks ◽  
Matthieu Palayret ◽  
David Klenerman ◽  
Steven F. Lee
Keyword(s):  
Single Molecule ◽  
Point Spread Function ◽  
Super Resolution ◽  
Point Spread ◽  
Spread Function ◽  
Resolution Imaging

scholarly journals Isotropic 3D Super Resolution Imaging with Self-Bending Point Spread Function

2013 ◽  
Vol 104 (2) ◽  
pp. 668a
Author(s):  
Shu Jia ◽  
Joshua C. Vaughan ◽  
Xiaowei Zhuang
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Point Spread ◽  
Spread Function ◽  
Resolution Imaging
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