Probing the Kinesin reaction cycle with a 2D optical force clamp

2003 ◽  
Author(s):  
C.L. Asbury ◽  
J.W. Shaevitz ◽  
M.J. Lang ◽  
S.M. Block
Keyword(s):  
Optical Force ◽  
Reaction Cycle ◽  
Force Clamp

scholarly journals Probing the kinesin reaction cycle with a 2D optical force clamp

2003 ◽  
Vol 100 (5) ◽  
pp. 2351-2356 ◽  
Author(s):  
S. M. Block ◽  
C. L. Asbury ◽  
J. W. Shaevitz ◽  
M. J. Lang
Keyword(s):  
Optical Force ◽  
Reaction Cycle ◽  
Force Clamp

High bandwidth optical force clamp for investigation of molecular motor motion

2013 ◽  
Vol 103 (15) ◽  
pp. 153703 ◽  
Author(s):  
Subhrajit Roychowdhury ◽  
Tanuj Aggarwal ◽  
Srinivasa Salapaka ◽  
Murti V. Salapaka
Keyword(s):  
Molecular Motor ◽  
Optical Force ◽  
High Bandwidth ◽  
Force Clamp

scholarly journals An Automated Two-Dimensional Optical Force Clamp for Single Molecule Studies

2002 ◽  
Vol 83 (1) ◽  
pp. 491-501 ◽  
Author(s):  
Matthew J. Lang ◽  
Charles L. Asbury ◽  
Joshua W. Shaevitz ◽  
Steven M. Block
Keyword(s):  
Single Molecule ◽  
Optical Force ◽  
Two Dimensional ◽  
Single Molecule Studies ◽  
Force Clamp

scholarly journals Passive All-Optical Force Clamp for High-Resolution Laser Trapping

2005 ◽  
Vol 95 (20) ◽  
Author(s):  
William J. Greenleaf ◽  
Michael T. Woodside ◽  
Elio A. Abbondanzieri ◽  
Steven M. Block
Keyword(s):  
High Resolution ◽  
Optical Force ◽  
Laser Trapping ◽  
All Optical ◽  
Force Clamp

scholarly journals Direct physical study of kinetochore–microtubule interactions by reconstitution and interrogation with an optical force clamp

Methods ◽  
2010 ◽  
Vol 51 (2) ◽  
pp. 242-250 ◽  
Author(s):  
Andrew D. Franck ◽  
Andrew F. Powers ◽  
Daniel R. Gestaut ◽  
Trisha N. Davis ◽  
Charles L. Asbury
Keyword(s):  
Optical Force ◽  
Physical Study ◽  
Force Clamp

Re-Programming Hydrogel Properties using a Fuel-driven Reaction Cycle

2019 ◽  
Author(s):  
Nishant Singh ◽  
Bruno Lainer ◽  
Georges Formon ◽  
Serena De Piccoli ◽  
Thomas Hermans
Keyword(s):  
Methionine Oxidation ◽  
Guanosine Triphosphate ◽  
Control Reaction ◽  
Reaction Cycle ◽  
Materials Properties ◽  
Assembly Kinetics ◽  
Control Assembly ◽  
Indispensable Tool ◽  
Reaction Cycles ◽  
Non Equilibrium

Nature uses catalysis as an indispensable tool to control assembly and reaction cycles in vital non-equilibrium supramolecular processes. For instance, enzymatic methionine oxidation regulates actin (dis)assembly, and catalytic guanosine triphosphate hydrolysis is found in tubulin (dis)assembly. Here we present a completely artificial reaction cycle which is driven by a chemical fuel that is catalytically obtained from a ‘pre-fuel’. The reaction cycle controls the disassembly and re-assembly of a hydrogel, where the rate of pre-fuel turnover dictates the morphology as well as the mechanical properties. By adding additional fresh aliquots of fuel and removing waste, the hydrogels can be re-programmed time after time. Overall, we show how catalysis can control fuel generation to control reaction / assembly kinetics and materials properties in life-like non-equilibrium systems.

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