Quantitative confocal fluorescence microscopy of dynamic processes by multifocal fluorescence correlation spectroscopy

2015 ◽  
Author(s):  
Aleksandar J. Krmpot ◽  
Stanko N. Nikolić ◽  
Marco Vitali ◽  
Dimitrios K. Papadopoulos ◽  
Sho Oasa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Confocal Fluorescence Microscopy ◽  
Dynamic Processes ◽  
Fluorescence Correlation ◽  
Confocal Fluorescence

How To Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy

Langmuir ◽  
2003 ◽  
Vol 19 (10) ◽  
pp. 4120-4126 ◽  
Author(s):  
A. Benda ◽  
M. Beneš ◽  
V. Mareček ◽  
A. Lhotský ◽  
W. Th. Hermens ◽  
...  
Keyword(s):  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Confocal Fluorescence

Real-time observation of single molecules by confocal fluorescence microscopy

1995 ◽  
Vol 53 ◽  
pp. 60-61
Author(s):  
Shuming Nie ◽  
Daniel T. Chiu ◽  
Richard N. Zare
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Single Molecule ◽  
High Efficiency ◽  
Spherical Aberration ◽  
Correlation Spectroscopy ◽  
Optical Diffraction ◽  
Confocal Fluorescence Microscopy ◽  
Scanning Tunneling ◽  
Confocal Fluorescence

The ability to detect, identify, and manipulate individual molecules offer exciting possibilities in many fields, including chemical analysis, materials research, and the biological sciences. A particularly powerful approach is to combine the exquisite sensitivity of laser-induced fluorescence and the spatial localization and imaging capabilities of diffraction-limited or near-field optical microscopes. Unlike scanning tunneling microscopy (STM) and atomic force microscopy (AFM), which lack molecular specificity, optical spectroscopy and microscopy techniques can be used for real-time monitoring and molecular identification at nanometer dimensions or in ultrasmall volumes.We report the use of confocal fluorescence microscopy coupled with a diffraction-limit laser beam and a high-efficiency photodiode for real-time detection of single fluorescent molecules in solution at room temperature. Rigler and Eigen have also demonstrated single-molecule detection with a confocal microscope and fluorescence correlation spectroscopy. The probe (or sampling) volume is effectively an elongated cylinder, with its radius being determined by optical diffraction and length by spherical aberration.

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