Red blood cell micromanipulation with elliptical laser beam profile optical tweezers in different osmolarity conditions

2011 ◽  
Author(s):  
E. Spyratou ◽  
M. Makropoulou ◽  
A.A. Serafetinides
Keyword(s):  
Blood Cell ◽  
Laser Beam ◽  
Optical Tweezers ◽  
Red Blood Cell ◽  
Beam Profile ◽  
Laser Beam Profile

Red blood cell in the field of a beam of optical tweezers

2022 ◽  
Vol 52 (1) ◽  
pp. 22-27
Author(s):  
P B Ermolinskiy ◽  
A E Lugovtsov ◽  
A N Semenov ◽  
A V Priezzhev
Keyword(s):  
Blood Cell ◽  
Laser Beam ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Optical Trapping ◽  
Optical Trap ◽  
Beam Power ◽  
Deformation Properties

Abstract We consider the effect of a tightly focused laser beam with a wavelength of 1064 nm and a power from 10 to 160 mW on red blood cells during their optical trapping with optical tweezers. It is found that the shape of a red blood cell, which alters after optical trapping, ceases to change when the trapping duration is less than 5 min and the laser beam power is less than 60 mW. At a beam power above 80 mW, the red blood cell begins to fold at a trapping duration of about 1 min, and at powers above 100-150 mW, the red blood cell membrane ruptures in 1-3 min after optical trapping. It is also found that with repeated short-term capture of a red blood cell in an optical trap, the deformation properties of the membrane change: it becomes more rigid. The obtained results are important both for understanding the mechanisms of interaction of a laser beam with red blood cells and for optimising the technique of optical experiments, especially for measuring the deformation properties of a membrane using optical tweezers.

Study of optimal laser beam profile for laser induced thermal printing of organic film

2009 ◽  
Author(s):  
Jonghoon Yi ◽  
Kangin Lee ◽  
Kwangwon Lee ◽  
Lee Soon Park ◽  
Jin Hyuk Kwon
Keyword(s):  
Laser Beam ◽  
Beam Profile ◽  
Organic Film ◽  
Laser Beam Profile

scholarly journals Dynamic deformation of red blood cell
in Dual-trap Optical Tweezers

2010 ◽  
Vol 18 (10) ◽  
pp. 10462 ◽  
Author(s):  
Sebastien Rancourt-Grenier ◽  
Ming-Tzo Wei ◽  
Jar-Jin Bai ◽  
Arthur Chiou ◽  
Paul P. Bareil ◽  
...  
Keyword(s):  
Blood Cell ◽  
Optical Tweezers ◽  
Red Blood Cell ◽  
Dynamic Deformation

scholarly journals INFLUENCE OF LASER BEAM PROFILE ON LIGHT SCATTERING BY HUMAN SKIN DURING PHOTOMETRY BY ELLIPSOIDAL REFLECTORS

2018 ◽  
Vol 9 (1) ◽  
pp. 56-65 ◽  
Author(s):  
M. A. Bezuglyi ◽  
N. V. Bezuglaya ◽  
S. Kostuk
Keyword(s):  
Monte Carlo Simulation ◽  
Adipose Tissue ◽  
Laser Beam ◽  
Human Skin ◽  
Cross Section ◽  
Beam Profile ◽  
Laser Beam Profile ◽  
Reflected Light ◽  
Section Profile ◽  
Cross Section Profile

The correct accounting of laser emitter parameters for improvement of diagnostic authenticity of methods of optical biomedical diagnostic is important problem for applied biophotonic tasks. The purpose of the current research is estimation of influence of energy distribution profile in transversal section of laser beam on light scattering by human skin layers at photometry by ellipsoidal reflectors.Biomedical photometer with ellipsoidal reflectors for investigation of biological tissue specimens in transmitted and reflected light uses laser probing radiation with infinitely thin, Gauss-type and uniform cross-section profile. Distribution of beams with denoted profiles, which consist of 20 million photons with wavelength 632.8 nm, was modeled by using of Monte-Carlo simulation in human skin layers (corneous layer, epidermis, derma and adipose tissue) of various anatomic thickness and with ellipsoidal reflectors with focal parameter equal to 16.875 mm and eccentricity of 0.66.The modeling results represent that illuminance distribution in zones of photometric imaging is significantly influenced by the laser beam cross-section profile for various thickness of corneous layer and epidermis in transmitted and reflected light, and also derma in reflected light. Illuminance distribution for adipose tissue in reflected and transmitted light, and also derma in transmitted light, practically do not depend of laser beam profile for anatomic thicknesses, which are appropriate for human skin on various sections of body.There are represented results of modified Monte-Carlo simulation method for biomedical photometer with ellipsoidal reflectors during biometry of human skin layers. For highly scattered corneous layer and epidermis the illumination of middle and external rings of photometric images changes depending from the laser beam profile for more than 50 % in transmitted and 30 % in reflected light. For weakly scattering skin layers (derma and adipose layer) the influence of profile can be observed only for derma in reflected layer and is equal not more than 15 %. 

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