Vertical Differential Interference Contrast using SLMs

2011 ◽  
Author(s):  
Michael Warber ◽  
Malte Hasler ◽  
Tobias Haist ◽  
Wolfgang Osten
Keyword(s):  
Differential Interference Contrast

scholarly journals Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis

2003 ◽  
Vol 160 (5) ◽  
pp. 671-683 ◽  
Author(s):  
Alexey Khodjakov ◽  
Lily Copenagle ◽  
Michael B. Gordon ◽  
Duane A. Compton ◽  
Tarun M. Kapoor
Keyword(s):  
Three Dimensional ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Spindle Formation ◽  
Capture Mechanism ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy ◽  
Kinesin Eg5 ◽  
Mitotic Kinesin Eg5 ◽  
Dependent Mechanism

Near-simultaneous three-dimensional fluorescence/differential interference contrast microscopy was used to follow the behavior of microtubules and chromosomes in living α-tubulin/GFP-expressing cells after inhibition of the mitotic kinesin Eg5 with monastrol. Kinetochore fibers (K-fibers) were frequently observed forming in association with chromosomes both during monastrol treatment and after monastrol removal. Surprisingly, these K-fibers were oriented away from, and not directly connected to, centrosomes and incorporated into the spindle by the sliding of their distal ends toward centrosomes via a NuMA-dependent mechanism. Similar preformed K-fibers were also observed during spindle formation in untreated cells. In addition, upon monastrol removal, centrosomes established a transient chromosome-free bipolar array whose orientation specified the axis along which chromosomes segregated. We propose that the capture and incorporation of preformed K-fibers complements the microtubule plus-end capture mechanism and contributes to spindle formation in vertebrates.

scholarly journals Calyptospora sp. in Brachyplatystoma vaillantii trapped at the Vigia, State of Pará, Brazil

2012 ◽  
Vol 21 (2) ◽  
pp. 176-178 ◽  
Author(s):  
Moacir Cerqueira da Silva ◽  
Sérgio Carmona da São Clemente ◽  
José Antônio Picanço Júnior ◽  
Michele Velasco Oliveira da Silva
Keyword(s):  
Light Microscopy ◽  
Coastal Region ◽  
Differential Interference Contrast ◽  
First Occurrence ◽  
City State ◽  
Hepatic Coccidiosis

The article describes the first occurrence of hepatic coccidiosis in catfish of the species Brachyplatystoma vaillantii, captured in the coastal region of the Vigia city, state of Pará, Brazil, caused by species of the genus Calyptospora, family Calyptosporidae. Thirty specimens of piramutabas were examined where 60% were infected with liver location, featuring numerous mature and immature oocysts, grouped or isolated, with four sporocysts in pyriform shape. They were described on their morphology and dimensions of the oocysts and sporocysts, obtained from light microscopy and differential interference contrast.

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